Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PYW05_RS27595 Genome accession   NZ_CP118875
Coordinates   5969658..5971148 (+) Length   496 a.a.
NCBI ID   WP_010955755.1    Uniprot ID   Q88CE2
Organism   Pseudomonas putida strain KT2440 Design 1b post-ALE     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 5964658..5976148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW05_RS27575 - 5964960..5965046 (+) 87 Protein_5412 magnesium chelatase -
  PYW05_RS27580 - 5965672..5966160 (+) 489 WP_010955754.1 type VI secretion system tube protein Hcp -
  PYW05_RS27585 - 5966170..5967146 (+) 977 Protein_5414 hypothetical protein -
  PYW05_RS27590 - 5968239..5968514 (+) 276 WP_061405724.1 bacteriocin -
  PYW05_RS27595 comM 5969658..5971148 (+) 1491 WP_010955755.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  PYW05_RS27600 - 5971217..5971585 (-) 369 WP_010955756.1 response regulator transcription factor -
  PYW05_RS27605 - 5971582..5972574 (-) 993 WP_049588349.1 response regulator -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 52991.88 Da        Isoelectric Point: 7.7100

>NTDB_id=796079 PYW05_RS27595 WP_010955755.1 5969658..5971148(+) (comM) [Pseudomonas putida strain KT2440 Design 1b post-ALE]
MSLALVHSRAQVGVQAPAVSVETHLANGLPHLTLVGLPETTVKESKDRVRSAIVNSGLKYPQRRITQNLAPADLPKDGGR
YDLAIALGILAADGQVPIATLTDMECLGELALSGKLRPVQGVLPAALAAREAGRALVVPRENAEEASLAGGLVVYAVGHL
LELVAHLNGQVPLPPYAANGLILQQRPYPDLSEVQGQLAAKRALLLAAAGAHNLLFTGPPGTGKTLLASRLPGLLPPLDE
HEALEVAAIQSVSGKAPLNSWPQRPFRHPHHSASGPALVGGSSRPQPGEITLAHHGVLFLDELPEFERRVLEVLREPLES
GEIVIARARDKVRFPARFQLVAAMNPCPCGYLGDPTGRCRCSTEQIARYRNKLSGPLLDRIDLHLTVARESTTLNNQPCG
ETSADVAARVAEARELQHRRQGCANAFLDLEGLRHHCGLVAADQVWLEGACERLTLSLRAAHRLLKVARTLADLEGCETI
GRAHLAEALQYRPGSS

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=796079 PYW05_RS27595 WP_010955755.1 5969658..5971148(+) (comM) [Pseudomonas putida strain KT2440 Design 1b post-ALE]
ATGTCCCTAGCCCTCGTCCACAGCCGCGCCCAGGTAGGCGTGCAGGCACCGGCGGTCAGCGTCGAAACCCACCTGGCCAA
CGGCCTGCCTCATCTCACCCTGGTCGGCCTGCCGGAAACCACGGTCAAGGAAAGCAAGGACCGGGTGCGCAGCGCCATCG
TCAACTCCGGGCTGAAGTACCCACAGCGGCGCATTACCCAGAACCTCGCCCCCGCCGACCTGCCCAAGGATGGCGGCCGA
TATGACCTGGCCATCGCCCTGGGCATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCT
GGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCC
GGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTG
CTGGAGTTGGTCGCCCACCTGAACGGCCAGGTGCCGCTACCGCCCTACGCCGCCAACGGTTTGATCCTGCAGCAACGGCC
CTACCCGGACCTGAGCGAAGTGCAGGGGCAACTGGCAGCCAAGCGTGCCTTGCTGCTGGCTGCGGCCGGTGCGCACAACC
TGCTGTTCACCGGGCCGCCCGGCACCGGCAAGACCTTGCTCGCCAGCCGCCTTCCCGGGCTGCTGCCACCGCTGGACGAG
CACGAAGCGCTGGAAGTGGCGGCAATCCAGTCGGTCAGCGGGAAAGCGCCGCTGAACAGCTGGCCACAGCGACCCTTTCG
CCACCCCCATCACTCTGCGTCCGGCCCGGCGCTGGTCGGTGGCAGTAGCCGACCGCAACCAGGTGAAATCACGCTCGCCC
ATCACGGCGTGTTGTTTCTCGACGAACTTCCGGAGTTTGAGCGGCGCGTACTGGAAGTGCTTCGCGAGCCACTGGAGTCA
GGTGAAATCGTGATTGCCCGTGCCCGCGACAAAGTGCGTTTCCCCGCGCGCTTCCAGCTGGTGGCGGCGATGAACCCCTG
CCCTTGCGGCTACCTGGGCGACCCCACTGGCCGCTGCCGCTGCAGCACCGAGCAGATCGCGCGCTATCGCAACAAGCTGT
CCGGGCCACTGCTGGACCGCATCGACCTGCACCTGACCGTGGCTCGCGAGAGCACTACATTGAACAACCAGCCTTGTGGT
GAAACCAGTGCCGATGTCGCCGCCAGGGTGGCTGAAGCGCGGGAGCTGCAGCACAGGCGACAAGGCTGTGCGAATGCATT
TCTCGACCTTGAAGGTTTAAGGCATCACTGTGGGTTGGTAGCGGCGGACCAAGTCTGGCTGGAAGGGGCCTGCGAGCGGC
TGACCTTGTCGCTACGGGCGGCGCACCGGTTGTTGAAGGTGGCGCGGACACTGGCGGACCTGGAGGGATGCGAGACGATT
GGCCGGGCGCACCTGGCCGAGGCCCTGCAGTACCGACCGGGGAGCAGTTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(190-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q88CE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

55.2

100

0.556
  comM Vibrio campbellii strain DS40M4

55.645

100

0.556
  comM Vibrio cholerae strain A1552

55.354

99.798

0.552
  comM Glaesserella parasuis strain SC1401

54.092

100

0.546
  comM Legionella pneumophila str. Paris

48.992

100

0.49
  comM Legionella pneumophila strain ERS1305867

48.992

100

0.49
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

47.41

100

0.48