Detailed information NTG0079465

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SKTS_RS04850
• Genome accession: NZ_AP022853
• Coordinates: 948645..949760 (-)
• Length: 371 a.a.
• NCBI ID: WP_198420425.1
• Uniprot ID: A0A6F8VA93
• Organism: Sulfurimicrobium lacus strain skT11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 943645..954760

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SKTS_RS04825 (SKTS_09420)	nifN	943744..945105 (-)	1362	WP_173061126.1	nitrogenase iron-molybdenum cofactor biosynthesis protein NifN	-
SKTS_RS04830 (SKTS_09430)	nifE	945163..946584 (-)	1422	WP_173061129.1	nitrogenase iron-molybdenum cofactor biosynthesis protein NifE	-
SKTS_RS04835 (SKTS_09440)	-	946828..947499 (+)	672	WP_173061132.1	ankyrin repeat domain-containing protein	-
SKTS_RS04840 (SKTS_09450)	-	947599..947949 (+)	351	WP_173061135.1	DMT family protein	-
SKTS_RS04845 (SKTS_09460)	-	947974..948570 (-)	597	WP_173061139.1	glycine zipper 2TM domain-containing protein	-
SKTS_RS04850 (SKTS_09470)	pilU	948645..949760 (-)	1116	WP_198420425.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SKTS_RS04855 (SKTS_09480)	-	950111..950851 (-)	741	WP_173061142.1	LytTR family DNA-binding domain-containing protein	-
SKTS_RS04860 (SKTS_09490)	-	950848..951831 (-)	984	WP_173061144.1	histidine kinase	-
SKTS_RS04865 (SKTS_09500)	-	951853..953052 (-)	1200	WP_173061146.1	hypothetical protein	-
SKTS_RS04870 (SKTS_09510)	-	953049..953786 (-)	738	WP_173061148.1	outer membrane lipoprotein-sorting protein	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41174.59 Da
• Isoelectric Point: 6.9135

>NTDB_id=79465 SKTS_RS04850 WP_198420425.1 948645..949760(-) (pilU) [Sulfurimicrobium lacus strain skT11]
MTDIQELLKLMVERGASDLFISAEISPRMKIQGETLPVNLPAHRRQGDEKDDLKTHIPWPALKPGESKKIAYELMTSAQR
REFEQTKECNFALASDHTGRYRVNVYYQRGEVSVAVRLIKADIPTFDVLGLPPQAAQLSMLKRGLVLMVGAAGTGKSTTL
AAMVGHRTKMVDGRIITIEDPIEFLFTHGKSMVEQREVGVDTLTFGDALRNAMREAPDVIVIGEIRDRETAQHAIMYAEA
GALCISTLHSNNANQAVERLINFFPEDARKQVLMDLSLNLKGVISQRLIPGMEGGLVLATEVLLQSAYSSDLIQKGQIDV
LKDAIKKGTEVGMQLFDDCLYKLYKARKISEQEALHNADSRTDLALKIKLS

Nucleotide

• Length: 1116 bp

>NTDB_id=79465 SKTS_RS04850 WP_198420425.1 948645..949760(-) (pilU) [Sulfurimicrobium lacus strain skT11]
ATGACTGATATCCAGGAACTCCTGAAATTAATGGTCGAGCGTGGGGCATCGGACCTTTTTATCTCGGCGGAAATCTCTCC
CCGCATGAAAATACAGGGGGAAACGCTCCCCGTTAACCTTCCCGCGCATCGCCGTCAGGGTGATGAGAAAGATGATCTCA
AAACCCATATTCCCTGGCCTGCACTCAAACCGGGAGAATCCAAGAAAATCGCTTATGAGCTGATGACCAGCGCGCAACGC
CGGGAGTTCGAGCAAACCAAAGAGTGCAACTTTGCCCTGGCTTCGGACCACACCGGCCGCTACCGCGTGAACGTCTACTA
CCAGCGCGGCGAGGTGTCGGTAGCGGTTCGACTGATCAAGGCGGATATCCCCACTTTCGACGTGCTCGGCCTGCCGCCTC
AGGCGGCGCAGCTCTCAATGCTCAAACGCGGCCTGGTGCTGATGGTCGGCGCGGCAGGTACCGGGAAAAGCACCACCCTG
GCGGCCATGGTCGGGCACCGGACCAAAATGGTGGATGGCCGCATCATCACCATCGAGGACCCGATCGAATTCCTGTTCAC
GCATGGGAAGTCGATGGTCGAGCAAAGGGAAGTCGGGGTAGACACACTGACATTCGGCGATGCGTTGCGAAATGCGATGC
GCGAAGCGCCGGACGTGATCGTCATCGGCGAGATCCGGGACCGGGAGACGGCCCAGCACGCGATCATGTACGCGGAAGCC
GGCGCGCTGTGTATTTCCACATTGCACTCCAACAACGCGAATCAGGCAGTCGAACGACTGATAAACTTCTTTCCGGAAGA
TGCGCGCAAGCAGGTTTTGATGGATCTGTCGCTCAACCTCAAGGGCGTGATTTCTCAGAGGCTGATTCCCGGCATGGAAG
GCGGCCTGGTGCTGGCGACGGAAGTTCTGCTGCAATCCGCCTATTCCTCGGACCTGATCCAGAAAGGCCAGATCGACGTT
CTGAAAGATGCCATCAAAAAAGGCACGGAAGTCGGCATGCAGTTATTCGACGATTGCCTGTACAAGCTCTACAAGGCGCG
CAAGATATCGGAGCAGGAAGCTTTGCACAATGCGGATTCGAGAACCGACCTGGCGCTCAAGATCAAGCTGTCGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-289)

3D structure

• AlphaFold DB: A0A6F8VA93

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	48.78	99.461	0.485
pilU	Acinetobacter baylyi ADP1	45.553	100	0.456
pilU	Vibrio cholerae strain A1552	45.109	99.191	0.447
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	37.815	96.226	0.364