Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HHSLTHF2_RS09830 Genome accession   NZ_AP022843
Coordinates   2050055..2051767 (+) Length   570 a.a.
NCBI ID   WP_172420904.1    Uniprot ID   A0A6F8U561
Organism   Halomonas hydrothermalis strain Slthf2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2045055..2056767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHSLTHF2_RS09795 (HHSLTHF2_19280) - 2045201..2045935 (+) 735 WP_172420898.1 hypothetical protein -
  HHSLTHF2_RS09800 (HHSLTHF2_19290) - 2045925..2046197 (+) 273 WP_172420899.1 hypothetical protein -
  HHSLTHF2_RS09805 (HHSLTHF2_19300) - 2046210..2046686 (+) 477 WP_172420900.1 hypothetical protein -
  HHSLTHF2_RS09815 (HHSLTHF2_19320) - 2047886..2048695 (-) 810 WP_172420901.1 inositol monophosphatase family protein -
  HHSLTHF2_RS09820 (HHSLTHF2_19330) - 2048786..2049241 (-) 456 WP_172420902.1 anti-virulence regulator CigR family protein -
  HHSLTHF2_RS19150 (HHSLTHF2_19340) - 2049407..2049799 (-) 393 WP_172420903.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HHSLTHF2_RS09830 (HHSLTHF2_19350) pilB 2050055..2051767 (+) 1713 WP_172420904.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HHSLTHF2_RS09835 (HHSLTHF2_19360) - 2051774..2053003 (+) 1230 WP_197915497.1 type II secretion system F family protein -
  HHSLTHF2_RS09840 pilD 2053059..2053904 (+) 846 WP_172420905.1 A24 family peptidase Machinery gene
  HHSLTHF2_RS09845 (HHSLTHF2_19380) coaE 2053945..2054541 (+) 597 WP_172420906.1 dephospho-CoA kinase -
  HHSLTHF2_RS09850 (HHSLTHF2_19390) yacG 2054581..2054814 (+) 234 WP_172420907.1 DNA gyrase inhibitor YacG -
  HHSLTHF2_RS09855 (HHSLTHF2_19400) - 2054828..2055700 (+) 873 WP_172420908.1 DNA replication terminus site-binding protein -
  HHSLTHF2_RS09860 (HHSLTHF2_19410) - 2055732..2056073 (-) 342 WP_125748130.1 hypothetical protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62569.67 Da        Isoelectric Point: 5.6637

>NTDB_id=79375 HHSLTHF2_RS09830 WP_172420904.1 2050055..2051767(+) (pilB) [Halomonas hydrothermalis strain Slthf2]
MHSGLHGIAKRLVENGELTDAQATVAENTATELDVSLLQHLVDSGLVESGAAAQAAAWEFGLPVIDLDAIRINALPPAND
YPVKLLERLEILPLARHEHRLTVAVPYPATLTQLDELQFATGLSIDGVLAPVDQLKSTLTQYLAQNERSMLDELDNVDDA
ISALNIVQQEEDEATLDKATKESEDAPVVKFVNKILLDAINRGASDIHFEPYETLYRVRFRIDGMLFEIARPPFALRHRI
AARVKIMSRLDISERRLPQDGAIKLQLSRTHSIDFRVNSLPTVFGEKIVLRLLDPTTAQLSIEQLGFSAEQQAVYEHALK
KPQGMILVTGPTGSGKTVTLYTGINILNQIERNICTAEDPVEIKVSGVNQVNVLPKIGLNFASALRAFLRQDPDVVMVGE
IRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGIASYNIASSVSLIIAQRLARKLCSLCKIPAQIPAEALRQA
GFSQQDIRNGTIYSAVGCKHCTQGYKGRIGIYEVVPITDAMRQLIMRDGDALNIDQQARREGYPSLYQRCLSRVLDGSTS
LAEINRISKE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=79375 HHSLTHF2_RS09830 WP_172420904.1 2050055..2051767(+) (pilB) [Halomonas hydrothermalis strain Slthf2]
ATGCATAGCGGCCTACACGGTATCGCTAAACGGCTGGTTGAAAATGGCGAGCTGACCGATGCTCAGGCGACTGTCGCGGA
AAACACAGCAACAGAGCTTGATGTGTCGTTGTTACAGCACTTGGTTGATAGCGGGCTAGTAGAATCAGGCGCAGCAGCGC
AGGCCGCTGCCTGGGAGTTTGGCTTGCCGGTCATTGACCTAGATGCCATTCGTATAAATGCGCTGCCACCCGCCAACGAC
TATCCCGTTAAATTATTAGAGCGTCTAGAGATTCTTCCCCTGGCACGCCATGAGCACCGACTGACGGTAGCTGTACCCTA
CCCGGCAACGCTAACCCAGTTAGATGAGCTGCAATTCGCTACCGGGCTAAGCATCGATGGAGTACTGGCTCCCGTTGATC
AACTTAAAAGCACTCTAACGCAGTATCTAGCGCAAAATGAGCGCAGCATGCTAGATGAATTAGACAACGTAGATGATGCG
ATCAGCGCGTTGAATATTGTTCAGCAGGAGGAAGATGAGGCAACTTTAGACAAGGCAACAAAAGAGAGTGAAGATGCCCC
CGTCGTCAAATTCGTCAATAAAATACTCTTAGATGCTATCAACCGCGGCGCTTCCGATATTCACTTTGAGCCTTATGAAA
CTCTGTACCGGGTGCGCTTTCGTATTGATGGCATGCTGTTTGAAATTGCCCGCCCACCTTTTGCGCTTCGGCACCGCATT
GCGGCTCGGGTTAAAATCATGTCTCGGTTAGACATCTCTGAGCGGCGTCTGCCCCAGGATGGAGCAATCAAGCTGCAGCT
TTCACGCACTCACTCAATTGATTTTCGAGTGAACTCACTGCCCACTGTGTTTGGTGAAAAAATAGTACTGCGCTTGCTAG
ATCCTACCACTGCTCAACTCTCTATCGAACAGCTAGGCTTTAGCGCTGAACAGCAGGCCGTTTATGAACACGCGCTTAAG
AAGCCTCAAGGAATGATTCTGGTTACCGGGCCTACGGGAAGCGGCAAAACAGTGACGCTTTATACAGGCATCAACATTCT
TAACCAAATTGAGCGCAATATCTGCACCGCTGAAGACCCGGTAGAAATTAAAGTGTCGGGGGTCAATCAGGTCAACGTCC
TTCCTAAAATAGGTCTGAACTTCGCTAGTGCTCTACGTGCCTTTTTACGCCAAGATCCCGATGTCGTTATGGTAGGCGAA
ATTCGTGACTTGGAAACCGCCGAAATTGCCGTGAAAGCAGCCCAAACGGGTCACTTAGTGCTATCTACCGTGCACACTAA
CTCCGCCGCTGAAACACTAACCCGCTTAGCAAATATGGGCATCGCATCCTATAACATCGCCAGCTCAGTTAGCTTGATCA
TTGCCCAACGCTTGGCACGCAAGCTATGCAGCCTATGCAAAATCCCCGCACAGATTCCTGCCGAAGCCCTGCGTCAGGCT
GGCTTTAGCCAGCAGGACATTCGCAACGGCACGATTTATTCAGCCGTCGGCTGTAAACACTGCACCCAGGGCTATAAAGG
TCGTATTGGTATCTATGAAGTGGTGCCCATTACTGACGCCATGCGCCAGTTGATTATGCGTGATGGTGATGCGCTGAATA
TCGATCAGCAAGCACGCCGCGAAGGTTACCCTAGCCTTTATCAACGCTGCCTAAGTCGTGTGCTGGATGGCAGCACTAGC
TTGGCAGAAATTAACCGCATCAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-147)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6F8U561

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.327

99.123

0.509
  pilB Acinetobacter baylyi ADP1

50

100

0.5
  pilB Acinetobacter baumannii D1279779

49.479

100

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

47.902

100

0.481
  pilB Vibrio cholerae strain A1552

48.825

97.018

0.474
  pilB Vibrio campbellii strain DS40M4

49.071

94.386

0.463
  pilF Neisseria gonorrhoeae MS11

44.128

98.596

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.583

92.632

0.367

Multiple sequence alignment