Detailed information NTG0793118

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PWA59_RS13680
• Genome accession: NZ_CP118541
• Coordinates: 2742106..2742720 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain HC-5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2737106..2747720

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWA59_RS13650 (PWA59_13650)	rpsT	2737409..2737675 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PWA59_RS13655 (PWA59_13655)	holA	2737692..2738733 (-)	1042	Protein_2643	DNA polymerase III subunit delta	-
PWA59_RS13660 (PWA59_13660)	-	2738773..2738924 (-)	152	Protein_2644	hypothetical protein	-
PWA59_RS13665 (PWA59_13665)	-	2738965..2739099 (+)	135	WP_003152870.1	YqzM family protein	-
PWA59_RS13670 (PWA59_13670)	comEC	2739118..2741469 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PWA59_RS13675 (PWA59_13675)	-	2741470..2742039 (-)	570	WP_003152868.1	ComE operon protein 2	-
PWA59_RS13680 (PWA59_13680)	comEA	2742106..2742720 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
PWA59_RS13685 (PWA59_13685)	comER	2742779..2743600 (+)	822	WP_014418420.1	late competence protein ComER	-
PWA59_RS13690 (PWA59_13690)	-	2743669..2744406 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
PWA59_RS13695 (PWA59_13695)	rsfS	2744403..2744759 (-)	357	WP_007408260.1	ribosome silencing factor	-
PWA59_RS13700 (PWA59_13700)	yqeK	2744778..2745338 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PWA59_RS13705 (PWA59_13705)	-	2745328..2745897 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
PWA59_RS13710 (PWA59_13710)	yhbY	2745908..2746198 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PWA59_RS13715 (PWA59_13715)	aroE	2746192..2747034 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=793118 PWA59_RS13680 WP_014418419.1 2742106..2742720(-) (comEA) [Bacillus velezensis strain HC-5]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=793118 PWA59_RS13680 WP_014418419.1 2742106..2742720(-) (comEA) [Bacillus velezensis strain HC-5]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363