Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   Q7631_RS22430 Genome accession   NZ_CP131685
Coordinates   4620064..4621557 (-) Length   497 a.a.
NCBI ID   WP_033547624.1    Uniprot ID   -
Organism   Escherichia coli strain 2016C04-116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4615064..4626557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7631_RS22400 (Q7631_22375) gspK 4615858..4616835 (-) 978 WP_000631647.1 type II secretion system minor pseudopilin GspK -
  Q7631_RS22405 (Q7631_22380) gspJ 4616832..4617437 (-) 606 WP_011579195.1 type II secretion system minor pseudopilin GspJ -
  Q7631_RS22410 (Q7631_22385) gspI 4617434..4617805 (-) 372 WP_000820119.1 type II secretion system minor pseudopilin GspI -
  Q7631_RS22415 (Q7631_22390) gspH 4617802..4618365 (-) 564 WP_001115150.1 type II secretion system minor pseudopilin GspH -
  Q7631_RS22420 (Q7631_22395) gspG 4618369..4618824 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  Q7631_RS22425 (Q7631_22400) gspF 4618841..4620064 (-) 1224 WP_001173427.1 type II secretion system inner membrane protein GspF -
  Q7631_RS22430 (Q7631_22405) pilB/pilB1 4620064..4621557 (-) 1494 WP_033547624.1 type II secretion system ATPase GspE Machinery gene
  Q7631_RS22435 (Q7631_22410) gspD 4621557..4623617 (-) 2061 WP_033560308.1 type II secretion system secretin GspD -
  Q7631_RS22440 (Q7631_22415) gspC 4623647..4624606 (-) 960 WP_000135085.1 type II secretion system protein GspC -
  Q7631_RS22445 (Q7631_22420) gspS2 4624624..4625034 (-) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  Q7631_RS22450 (Q7631_22425) pilD 4625100..4625909 (-) 810 WP_000895866.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55154.29 Da        Isoelectric Point: 5.9081

>NTDB_id=790886 Q7631_RS22430 WP_033547624.1 4620064..4621557(-) (pilB/pilB1) [Escherichia coli strain 2016C04-116]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETALDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=790886 Q7631_RS22430 WP_033547624.1 4620064..4621557(-) (pilB/pilB1) [Escherichia coli strain 2016C04-116]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTACCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACACTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTATGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCGCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Legionella pneumophila strain ERS1305867

42.398

93.964

0.398
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Acinetobacter baumannii D1279779

46.115

80.282

0.37
  pilB Vibrio cholerae strain A1552

45.707

79.678

0.364
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362