Detailed information NTG0787995

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PUO96_RS22915
• Genome accession: NZ_CP117853
• Coordinates: 4875330..4876127 (+)
• Length: 265 a.a.
• NCBI ID: WP_064405744.1
• Uniprot ID: -
• Organism: Klebsiella michiganensis strain 2020CK-00215
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4870330..4881127

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUO96_RS22880 (PUO96_22880)	gspH	4870471..4870983 (+)	513	WP_064405737.1	type II secretion system minor pseudopilin GspH	-
PUO96_RS22885 (PUO96_22885)	gspI	4870980..4871345 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
PUO96_RS22890 (PUO96_22890)	gspJ	4871342..4871938 (+)	597	WP_064405739.1	type II secretion system minor pseudopilin GspJ	-
PUO96_RS22895 (PUO96_22895)	gspK	4871935..4872915 (+)	981	WP_064405740.1	type II secretion system minor pseudopilin GspK	-
PUO96_RS22900 (PUO96_22900)	gspL	4872908..4874101 (+)	1194	WP_064374754.1	type II secretion system protein GspL	-
PUO96_RS22905 (PUO96_22905)	gspM	4874094..4874579 (+)	486	WP_032748031.1	type II secretion system protein GspM	-
PUO96_RS22910 (PUO96_22910)	-	4874569..4875327 (+)	759	WP_064405742.1	type II secretion system protein N	-
PUO96_RS22915 (PUO96_22915)	pilD	4875330..4876127 (+)	798	WP_064405744.1	prepilin peptidase	Machinery gene
PUO96_RS22920 (PUO96_22920)	hrpB	4876223..4878652 (-)	2430	WP_064358587.1	ATP-dependent helicase HrpB	-
PUO96_RS22925 (PUO96_22925)	thpR	4878725..4879261 (+)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
PUO96_RS22930 (PUO96_22930)	sfsA	4879261..4879977 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
PUO96_RS22935 (PUO96_22935)	dksA	4880142..4880597 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29136.74 Da
• Isoelectric Point: 8.3410

>NTDB_id=787995 PUO96_RS22915 WP_064405744.1 4875330..4876127(+) (pilD) [Klebsiella michiganensis strain 2020CK-00215]
MENIALLPEFAAQFPLIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLTFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMAGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=787995 PUO96_RS22915 WP_064405744.1 4875330..4876127(+) (pilD) [Klebsiella michiganensis strain 2020CK-00215]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATTCCCGTTAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAACTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGTCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGCCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCGGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (124-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.192	98.491	0.494
pilD	Neisseria gonorrhoeae MS11	47.431	95.472	0.453
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434