Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PUP35_RS18195 Genome accession   NZ_CP117838
Coordinates   3854097..3855077 (-) Length   326 a.a.
NCBI ID   WP_274240780.1    Uniprot ID   -
Organism   Enterobacter sp. S-33     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3849097..3860077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP35_RS18165 - 3849974..3850471 (+) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -
  PUP35_RS18170 endA 3850566..3851273 (+) 708 WP_274240778.1 deoxyribonuclease I -
  PUP35_RS18175 rsmE 3851325..3852056 (+) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PUP35_RS18180 gshB 3852076..3853023 (+) 948 WP_274240779.1 glutathione synthase -
  PUP35_RS18185 - 3853111..3853671 (+) 561 WP_020883695.1 YqgE/AlgH family protein -
  PUP35_RS18190 ruvX 3853671..3854087 (+) 417 WP_127732267.1 Holliday junction resolvase RuvX -
  PUP35_RS18195 pilT 3854097..3855077 (-) 981 WP_274240780.1 type IV pilus twitching motility protein PilT Machinery gene
  PUP35_RS18200 - 3855095..3855796 (+) 702 WP_274240781.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUP35_RS18205 - 3855818..3856384 (+) 567 WP_111963226.1 YggT family protein -
  PUP35_RS18210 yggU 3856381..3856677 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  PUP35_RS18215 - 3856681..3857274 (+) 594 WP_136195092.1 XTP/dITP diphosphatase -
  PUP35_RS18220 hemW 3857267..3858409 (+) 1143 WP_274240782.1 radical SAM family heme chaperone HemW -
  PUP35_RS18225 - 3858618..3859334 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  PUP35_RS18230 - 3859391..3859717 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35523.50 Da        Isoelectric Point: 6.6962

>NTDB_id=787885 PUP35_RS18195 WP_274240780.1 3854097..3855077(-) (pilT) [Enterobacter sp. S-33]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKAWLNDEQQGTWWANGQVDFAATVTGGQRLRG
SAFKQMRGVSLTLRLLPRTCPQLATLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETVRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGLQAGMQNFNQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=787885 PUP35_RS18195 WP_274240780.1 3854097..3855077(-) (pilT) [Enterobacter sp. S-33]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCTGACGTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCAAATGGACAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGTCTGCGCGGG
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTTTGACGCTGCGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTACGCTGGG
CGCGCCGCGGGCAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACCGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATTGGCCAGCACAGCCCGTCATTTGCCGACGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGGTCCGCCTGGCGCTAACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTCCGGGCAGTGCTGGCGCAGAAGCTGCTTCC
CGATCTGCAGGACGGCCGCGTCGCGCTGTATGAACTGCTGGTGAACACCGCTGCGGCGGCAAATCTTATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATCCAAACCGGTCTGCAGGCAGGAATGCAGAACTTTAACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362