Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PUP35_RS19460 Genome accession   NZ_CP117838
Coordinates   4095358..4096791 (+) Length   477 a.a.
NCBI ID   WP_111964974.1    Uniprot ID   -
Organism   Enterobacter sp. S-33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4090358..4101791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP35_RS19430 - 4090925..4091902 (+) 978 WP_039260412.1 calcium/sodium antiporter -
  PUP35_RS19435 kdsD 4091916..4092902 (+) 987 WP_032659705.1 arabinose-5-phosphate isomerase KdsD -
  PUP35_RS19440 kdsC 4092917..4093483 (+) 567 WP_274243315.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PUP35_RS19445 lptC 4093480..4094055 (+) 576 WP_115876356.1 LPS export ABC transporter periplasmic protein LptC -
  PUP35_RS19450 lptA 4094024..4094578 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PUP35_RS19455 lptB 4094585..4095310 (+) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  PUP35_RS19460 rpoN/rpoN1 4095358..4096791 (+) 1434 WP_111964974.1 RNA polymerase factor sigma-54 Machinery gene
  PUP35_RS19465 hpf 4096814..4097101 (+) 288 WP_111964975.1 ribosome hibernation promoting factor -
  PUP35_RS19470 ptsN 4097184..4097675 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PUP35_RS19475 rapZ 4097721..4098575 (+) 855 WP_008501442.1 RNase adapter RapZ -
  PUP35_RS19480 npr 4098572..4098844 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  PUP35_RS19485 mtgA 4098884..4099609 (-) 726 WP_127732416.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PUP35_RS19490 elbB 4099606..4100259 (-) 654 WP_274243317.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54139.91 Da        Isoelectric Point: 4.3332

>NTDB_id=787886 PUP35_RS19460 WP_111964974.1 4095358..4096791(+) (rpoN/rpoN1) [Enterobacter sp. S-33]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARVIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=787886 PUP35_RS19460 WP_111964974.1 4095358..4096791(+) (rpoN/rpoN1) [Enterobacter sp. S-33]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGACACTGAAGCGCTTGATACCGCCGACGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACGCCTTCCGGTACGCGTGCGGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACTCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTTGACGAT
ATTCTGGAAAGCATGGGCGACGAAGAGATAGAGCTTGAGGAAGTTGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTAGCCGCAAAAGATCTGCGCGACTGCCTGCTAATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCGTGATTATCAGCGATCACCTGGATCTGCTGGCGAACCACGATTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGATGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCACTCGATCCACGCCCGGGACAGTCAATCCAGACCAG
CGAGCCTGAGTACGTTATTCCGGACGTGCTGGTCAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTTGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCTTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCATAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCGCTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482