Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PUO95_RS10505 Genome accession   NZ_CP117823
Coordinates   2296716..2298449 (+) Length   577 a.a.
NCBI ID   WP_004090374.1    Uniprot ID   Q87AA1
Organism   Xylella fastidiosa subsp. fastidiosa strain ICMP 8742     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2291716..2303449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUO95_RS10485 (PUO95_10485) pilC 2292035..2293237 (-) 1203 WP_011098309.1 type II secretion system F family protein Machinery gene
  PUO95_RS10490 (PUO95_10490) - 2293678..2294124 (+) 447 WP_004090380.1 pilin -
  PUO95_RS10495 (PUO95_10495) - 2294370..2294597 (-) 228 WP_011098310.1 hypothetical protein -
  PUO95_RS10500 (PUO95_10500) - 2295433..2295873 (+) 441 WP_004090375.1 pilin -
  PUO95_RS10505 (PUO95_10505) pilB 2296716..2298449 (+) 1734 WP_004090374.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUO95_RS10510 (PUO95_10510) pilR 2298543..2299955 (-) 1413 WP_004090373.1 sigma-54 dependent transcriptional regulator Regulator
  PUO95_RS10515 (PUO95_10515) - 2300141..2301751 (-) 1611 WP_004090372.1 ATP-binding protein -
  PUO95_RS10520 (PUO95_10520) sucC 2302006..2303169 (+) 1164 WP_004090371.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62946.98 Da        Isoelectric Point: 5.9144

>NTDB_id=787791 PUO95_RS10505 WP_004090374.1 2296716..2298449(+) (pilB) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALD
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPAQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=787791 PUO95_RS10505 WP_004090374.1 2296716..2298449(+) (pilB) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
ATGAACACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAGGATGGCAGCATTGAGGA
AGCCACTGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TGAGTGCAGCAAAATTGGCCGCGGCCAATGCACTTGAGTTCGGGATGCCGCTACTGGATGTGTCCGCATTCGACCACAAT
CACAATGCAATGAACTTGGTCGACGAAGAATTACTGCAAAAGCATCGAGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACTCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGGCGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAT
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACAGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCGTCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTACTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTACTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTCTCACTTTA
CACTGCGTTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGTG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCATTACGTTCGTTTCTGCGCCAAGACCCGGAT
GTGATCATGGTCGGCGAAATCCGTGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACCCTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGCTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACTGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCACAGCGCATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87AA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.966

98.267

0.56
  pilB Acinetobacter baumannii D1279779

56.79

98.267

0.558
  pilB Legionella pneumophila strain ERS1305867

52.456

98.787

0.518
  pilF Neisseria gonorrhoeae MS11

50.264

98.614

0.496
  pilB Vibrio cholerae strain A1552

49.36

94.801

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

47.08

97.92

0.461
  pilB Vibrio campbellii strain DS40M4

46.303

98.44

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.993

92.894

0.362