Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   PUO95_RS10510 Genome accession   NZ_CP117823
Coordinates   2298543..2299955 (-) Length   470 a.a.
NCBI ID   WP_004090373.1    Uniprot ID   Q87AA0
Organism   Xylella fastidiosa subsp. fastidiosa strain ICMP 8742     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 2293543..2304955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUO95_RS10490 (PUO95_10490) - 2293678..2294124 (+) 447 WP_004090380.1 pilin -
  PUO95_RS10495 (PUO95_10495) - 2294370..2294597 (-) 228 WP_011098310.1 hypothetical protein -
  PUO95_RS10500 (PUO95_10500) - 2295433..2295873 (+) 441 WP_004090375.1 pilin -
  PUO95_RS10505 (PUO95_10505) pilB 2296716..2298449 (+) 1734 WP_004090374.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUO95_RS10510 (PUO95_10510) pilR 2298543..2299955 (-) 1413 WP_004090373.1 sigma-54 dependent transcriptional regulator Regulator
  PUO95_RS10515 (PUO95_10515) - 2300141..2301751 (-) 1611 WP_004090372.1 ATP-binding protein -
  PUO95_RS10520 (PUO95_10520) sucC 2302006..2303169 (+) 1164 WP_004090371.1 ADP-forming succinate--CoA ligase subunit beta -
  PUO95_RS10525 (PUO95_10525) sucD 2303198..2304070 (+) 873 WP_004090370.1 succinate--CoA ligase subunit alpha -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 51272.13 Da        Isoelectric Point: 7.2159

>NTDB_id=787792 PUO95_RS10510 WP_004090373.1 2298543..2299955(-) (pilR) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
MNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMRLPDGSGIELVKKIVRHYPRTPVA
MITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSPSQRLLGESSPMAQLRATIAKVAR
SQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGSFTGAHTDKPGLFQSAGGGTLFLD
EVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHDLYYRINVIEIRVPPLRERAGDLP
HLAAAIVARLAHTHGRAVPLLSPSALEALEHYSFPGNVRELENILERALALTEDDHIGAADLHLPQGSLHGQGLPLPLVP
TVLPIVPADASASVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITFRALRYKLKKLGME

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=787792 PUO95_RS10510 WP_004090373.1 2298543..2299955(-) (pilR) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
ATGAATGAATCCCGCAGTGCTCTGATTGTCGATGACGAGCGCGACATCCGTGAACTTTTGGTCCTTACGCTAGGACGTAT
GGGGCTGCGTATCAGAACGGCAGCTAATCTGGCACAAGCATACGCGTTACTTGAGTCTGACTCGTTCAATCTTTGTATCA
CCGACATGCGTTTACCTGACGGCAGTGGCATTGAGCTGGTGAAAAAGATTGTGCGTCATTATCCACGCACCCCGGTGGCC
ATGATCACTGCTTTTGGCAGTATCGAACTGGCGGTAGAGGCGCTCAAGGAAGGTGCTTTTGACTTTGTCAGCAAACCTGT
TGATCTGAACGTCCTGCGAGGCATGGTCCGTCATGCTTTGGAATTGAACAATCGTGAACGCTCTGTTGTGTCAAGTCAGC
CACAAAGCCCCAGCCAACGTCTCCTTGGTGAATCATCACCGATGGCTCAGCTTCGGGCCACCATCGCTAAAGTGGCGCGT
AGTCAAGCCCCGGTTTATATCCTTGGTGAATCAGGAGTTGGTAAAGAACTGGTTGCACGTACGATCCACGAACAGAGTGT
GCGTGCTGCTGGGGCGTTCGTGCCGGTCAACTGTGGTGCGATCCCTGTGGATCTTATGGAAAGCGAATTTTTCGGTCACA
AGAAAGGGAGTTTCACCGGTGCGCATACTGATAAGCCAGGTTTATTCCAAAGTGCTGGCGGCGGTACTTTATTCCTGGAT
GAAGTTGCCGAATTACCCTTGCACATGCAAGTTAAATTGCTGCGTGCGATTCAGGAGAAATCGATACGGCCAATCGGTGC
AACCGTTGAGGTACCAGTAGATGTGCGTATTTTATCGGCGACTCACCAAGATCTGAGTGACCTGATTGCCAATGGCCGCT
TCCGTCACGATTTGTACTACCGCATTAATGTAATCGAGATACGTGTTCCACCGCTACGTGAGCGTGCTGGCGATTTGCCG
CATCTTGCTGCAGCCATTGTGGCTCGCTTGGCACACACGCATGGGCGGGCGGTCCCGTTGCTTTCACCCTCCGCCTTGGA
GGCGTTAGAACATTATTCGTTCCCGGGTAATGTGCGTGAACTGGAGAACATTCTGGAGCGTGCTCTAGCACTGACTGAAG
ATGATCATATCGGTGCCGCGGATCTGCACCTGCCCCAGGGCAGTTTGCATGGACAGGGATTGCCGCTCCCGTTAGTGCCC
ACAGTTCTCCCGATTGTGCCTGCGGACGCATCAGCATCGGTGGAGGGTGAGAATACGCCGGCTGCCTTGCCCTCGTACAT
GGAGCAGCTGGAACGTGCTGCCATTCAAAAGGCGTTGGAAGAGAATCGCTGGAATAAAACAAAGACAGCCGCAAAACTTG
GCATCACTTTCCGTGCATTACGTTACAAACTAAAGAAGCTGGGTATGGAGTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(142-307)

Response regulator receiver domain (Response_reg)

(8-116)

Bacterial regulatory protein, Fis family (HTH_8)

(427-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87AA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

58.422

99.787

0.583
  pilR Acinetobacter baumannii strain A118

47.034

100

0.472