Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PT257_RS06960 Genome accession   NZ_CP117818
Coordinates   1562512..1564236 (+) Length   574 a.a.
NCBI ID   WP_011946523.1    Uniprot ID   -
Organism   Legionella pneumophila strain D5916     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1557512..1569236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT257_RS06940 (PT257_06940) - 1557865..1560387 (+) 2523 WP_061784267.1 GGDEF domain-containing phosphodiesterase -
  PT257_RS06945 (PT257_06945) - 1560389..1560958 (-) 570 WP_011213801.1 hypoxanthine-guanine phosphoribosyltransferase -
  PT257_RS06950 (PT257_06950) - 1561001..1561327 (-) 327 WP_011213802.1 DUF1820 family protein -
  PT257_RS06955 (PT257_06955) - 1561510..1562253 (-) 744 WP_011213803.1 methyltransferase domain-containing protein -
  PT257_RS06960 (PT257_06960) pilB 1562512..1564236 (+) 1725 WP_011946523.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PT257_RS06970 (PT257_06970) - 1564242..1565460 (+) 1219 Protein_1367 type II secretion system F family protein -
  PT257_RS06975 (PT257_06975) pilD 1565506..1566369 (+) 864 WP_011946525.1 A24 family peptidase Machinery gene
  PT257_RS06980 (PT257_06980) - 1566383..1567588 (-) 1206 WP_013101470.1 cyclic nucleotide-binding domain-containing protein -
  PT257_RS06985 (PT257_06985) - 1567696..1568289 (-) 594 WP_011213808.1 hypothetical protein -
  PT257_RS06990 (PT257_06990) - 1568305..1568760 (-) 456 WP_042233877.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62722.31 Da        Isoelectric Point: 5.5861

>NTDB_id=787729 PT257_RS06960 WP_011946523.1 1562512..1564236(+) (pilB) [Legionella pneumophila strain D5916]
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=787729 PT257_RS06960 WP_011946523.1 1562512..1564236(+) (pilB) [Legionella pneumophila strain D5916]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGACAAAACAAA
AGCAATTGAACTACACAAGTTGGCAGCAGCAGAAAAGATATCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCCATGTTGGATATTAATTGCATTGATGTGGGCACCATT
CCTGTCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGTCGCGGCACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAGGCTTCATTAAAGGAAATTCAATTTCATACCGGATTAAATACTCATGCTATAGTTG
TAGAAACAGATAAACTTAGTGCTCTGATTGATAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTAGAAGGTTTGGAAATAAGTGCTGATGATGAAGATCAAGATATTGATACTGCAACATCAGTAACTGA
TGATGCACCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAAC
CTTATGAACGAGAATATCGAATCAGGTACAGGCAAGATGGTATTTTACATGAGGTCGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAAGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGCACATGTCCAACCTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGCGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTCACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTGCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTGCGTTCGTTTCTAAGGCAAGATCCTGATATTATCATG
GTTGGTGAGATACGTGATCTTGAAACAGCAGAGATTGCTATAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAATATGGGAATACCTACTTTTAACATCGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGTGATGACTTTACCAATCAAGGC
TTGATTGAACTAGGTTTCAAGGAAGCTGATTTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGTGAGCAATGTACAAG
TGGTTATCGCGGGCGAGTTGGGTTGTTTGAGGTTTTACCGATGACAAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGTTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATCTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

100

100

1
  pilB Acinetobacter baylyi ADP1

49.828

100

0.505
  pilB Acinetobacter baumannii D1279779

50.794

98.78

0.502
  pilB Vibrio cholerae strain A1552

50.741

94.077

0.477
  pilF Neisseria gonorrhoeae MS11

47.173

98.606

0.465
  pilB Vibrio campbellii strain DS40M4

47.756

97.038

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

48.98

93.902

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

92.16

0.364
  pilF Thermus thermophilus HB27

38.848

93.728

0.364