Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PT257_RS04695 Genome accession   NZ_CP117818
Coordinates   1043453..1044517 (+) Length   354 a.a.
NCBI ID   WP_027266405.1    Uniprot ID   -
Organism   Legionella pneumophila strain D5916     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1038453..1049517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT257_RS04685 (PT257_04685) - 1039493..1041877 (-) 2385 WP_027266407.1 penicillin-binding protein 1A -
  PT257_RS04690 (PT257_04690) ravI 1042029..1043036 (-) 1008 WP_027266406.1 Dot/Icm T4SS effector RavI -
  PT257_RS04695 (PT257_04695) pilM 1043453..1044517 (+) 1065 WP_027266405.1 type IV pilus assembly protein PilM Machinery gene
  PT257_RS04700 (PT257_04700) pilN 1044521..1045069 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  PT257_RS04705 (PT257_04705) pilO 1045083..1045682 (+) 600 WP_011215096.1 type 4a pilus biogenesis protein PilO Machinery gene
  PT257_RS04710 (PT257_04710) pilP 1045679..1046263 (+) 585 WP_027266404.1 pilus assembly protein PilP Machinery gene
  PT257_RS04715 (PT257_04715) pilQ 1046267..1048363 (+) 2097 WP_061483600.1 type IV pilus secretin PilQ Machinery gene
  PT257_RS04720 (PT257_04720) aroK 1048810..1049337 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38790.81 Da        Isoelectric Point: 5.0367

>NTDB_id=787721 PT257_RS04695 WP_027266405.1 1043453..1044517(+) (pilM) [Legionella pneumophila strain D5916]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAITLKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=787721 PT257_RS04695 WP_027266405.1 1043453..1044517(+) (pilM) [Legionella pneumophila strain D5916]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTACCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

99.718

100

0.997
  comM Acinetobacter nosocomialis M2

42.373

100

0.424
  pilM Acinetobacter baumannii D1279779

42.09

100

0.421
  comM Acinetobacter baylyi ADP1

40.678

100

0.407