Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PT257_RS04705 Genome accession   NZ_CP117818
Coordinates   1045083..1045682 (+) Length   199 a.a.
NCBI ID   WP_011215096.1    Uniprot ID   -
Organism   Legionella pneumophila strain D5916     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1040083..1050682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT257_RS04690 (PT257_04690) ravI 1042029..1043036 (-) 1008 WP_027266406.1 Dot/Icm T4SS effector RavI -
  PT257_RS04695 (PT257_04695) pilM 1043453..1044517 (+) 1065 WP_027266405.1 type IV pilus assembly protein PilM Machinery gene
  PT257_RS04700 (PT257_04700) pilN 1044521..1045069 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  PT257_RS04705 (PT257_04705) pilO 1045083..1045682 (+) 600 WP_011215096.1 type 4a pilus biogenesis protein PilO Machinery gene
  PT257_RS04710 (PT257_04710) pilP 1045679..1046263 (+) 585 WP_027266404.1 pilus assembly protein PilP Machinery gene
  PT257_RS04715 (PT257_04715) pilQ 1046267..1048363 (+) 2097 WP_061483600.1 type IV pilus secretin PilQ Machinery gene
  PT257_RS04720 (PT257_04720) aroK 1048810..1049337 (+) 528 WP_010946667.1 shikimate kinase AroK -
  PT257_RS04725 (PT257_04725) aroB 1049324..1050433 (+) 1110 WP_013101269.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22482.18 Da        Isoelectric Point: 5.3369

>NTDB_id=787723 PT257_RS04705 WP_011215096.1 1045083..1045682(+) (pilO) [Legionella pneumophila strain D5916]
MDTVNLSELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEVALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=787723 PT257_RS04705 WP_011215096.1 1045083..1045682(+) (pilO) [Legionella pneumophila strain D5916]
ATGGACACGGTCAATTTAAGTGAATTAACTCTGGAGAATGTCGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTCATTTT
AGGTATCAGTATACTGATAATTGGATTAGGATATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAGTTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCTTATCGTAATCAGTTA
CAGTTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTCCCGGCAAAAAATGAAATGCCAGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATTGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTGGCTGAAATGAACAGA
ATAGTGACTTTGCATGAATTTAGTGTTGAGGGGGTGTCATCCGGGGATGGTAAAACGGTCTCTCAGGATGAATTGGTGAT
GGCTATCACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

98.995

100

0.99
  comO Acinetobacter baylyi ADP1

39.894

94.472

0.377
  pilO Acinetobacter baumannii D1279779

37.696

95.98

0.362