Detailed information NTG0786285

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSP57_RS02295
• Genome accession: NZ_CP117527
• Coordinates: 513834..514982 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain MF1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 508834..519982

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSP57_RS02270 (PSP57_02270)	-	509985..510281 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PSP57_RS02275 (PSP57_02275)	-	510278..510871 (-)	594	WP_003084544.1	YggT family protein	-
PSP57_RS02280 (PSP57_02280)	proC	510882..511703 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PSP57_RS02285 (PSP57_02285)	-	511715..512407 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PSP57_RS02290 (PSP57_02290)	pilT	512622..513656 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PSP57_RS02295 (PSP57_02295)	pilU	513834..514982 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PSP57_RS02300 (PSP57_02300)	czcD	514990..515889 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PSP57_RS02305 (PSP57_02305)	-	516044..516448 (+)	405	WP_003084560.1	NINE protein	-
PSP57_RS02310 (PSP57_02310)	-	516691..518064 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PSP57_RS02315 (PSP57_02315)	-	518061..519245 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=786285 PSP57_RS02295 WP_003110016.1 513834..514982(+) (pilU) [Pseudomonas aeruginosa strain MF1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=786285 PSP57_RS02295 WP_003110016.1 513834..514982(+) (pilU) [Pseudomonas aeruginosa strain MF1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382