Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   PSC78_RS12750 Genome accession   NZ_CP117508
Coordinates   2601613..2602911 (+) Length   432 a.a.
NCBI ID   WP_002368406.1    Uniprot ID   R3K7C6
Organism   Enterococcus faecalis strain CQJXZ21-077     
Function   repress competence development (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2561787..2601212 2601613..2602911 flank 401


Gene organization within MGE regions


Location: 2561787..2602911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSC78_RS12550 (PSC78_12550) - 2561787..2562467 (-) 681 WP_002346958.1 IS6-like element IS1216 family transposase -
  PSC78_RS12555 (PSC78_12555) tcrB 2562692..2564824 (-) 2133 WP_002346660.1 copper-translocating P-type ATPase TcrB -
  PSC78_RS12560 (PSC78_12560) tcrZ 2564963..2565169 (-) 207 WP_002335389.1 copper chaperone TcrZ -
  PSC78_RS12565 (PSC78_12565) - 2565254..2567719 (-) 2466 WP_002346959.1 heavy metal translocating P-type ATPase -
  PSC78_RS12570 (PSC78_12570) tcrY 2567732..2568187 (-) 456 WP_002294574.1 copper-responsive transcriptional repressor TcrY -
  PSC78_RS12575 (PSC78_12575) - 2568798..2569924 (+) 1127 WP_104681286.1 IS3 family transposase -
  PSC78_RS12580 (PSC78_12580) - 2569967..2570704 (+) 738 Protein_2458 ISL3 family transposase -
  PSC78_RS12585 (PSC78_12585) - 2570972..2571568 (-) 597 WP_079993992.1 YdhK family protein -
  PSC78_RS12590 (PSC78_12590) - 2571589..2571795 (-) 207 WP_002307659.1 hypothetical protein -
  PSC78_RS12595 (PSC78_12595) - 2571810..2573204 (-) 1395 WP_002307657.1 sensor histidine kinase -
  PSC78_RS12600 (PSC78_12600) - 2573204..2573884 (-) 681 WP_002294790.1 response regulator transcription factor -
  PSC78_RS12605 (PSC78_12605) - 2573912..2575495 (-) 1584 WP_033604471.1 multicopper oxidase family protein -
  PSC78_RS12610 (PSC78_12610) - 2575506..2575730 (-) 225 WP_002328408.1 heavy-metal-associated domain-containing protein -
  PSC78_RS12615 (PSC78_12615) - 2576172..2576438 (-) 267 Protein_2465 ISL3 family transposase -
  PSC78_RS12625 (PSC78_12625) - 2577805..2578866 (-) 1062 Protein_2467 ISL3 family transposase -
  PSC78_RS12630 (PSC78_12630) merA 2579026..2580921 (-) 1896 WP_002328410.1 mercury(II) reductase -
  PSC78_RS12635 (PSC78_12635) merR 2580950..2581342 (-) 393 WP_001863748.1 Hg(II)-responsive transcriptional regulator -
  PSC78_RS14625 - 2581687..2581761 (+) 75 WP_373415972.1 hypothetical protein -
  PSC78_RS12645 (PSC78_12645) - 2582067..2583208 (+) 1142 WP_096933284.1 IS3 family transposase -
  PSC78_RS12650 (PSC78_12650) - 2583720..2584625 (-) 906 WP_002328337.1 Ig-like domain-containing protein -
  PSC78_RS12655 (PSC78_12655) - 2584819..2586030 (-) 1212 WP_002328338.1 heavy metal translocating P-type ATPase -
  PSC78_RS12660 (PSC78_12660) - 2586014..2586379 (-) 366 WP_033604469.1 BlaI/MecI/CopY family transcriptional regulator -
  PSC78_RS12665 (PSC78_12665) - 2586395..2587009 (-) 615 WP_002319974.1 TIGR01906 family membrane protein -
  PSC78_RS12670 (PSC78_12670) lgt 2587165..2587968 (+) 804 WP_002328340.1 prolipoprotein diacylglyceryl transferase -
  PSC78_RS12675 (PSC78_12675) - 2587952..2588176 (+) 225 WP_002307648.1 DUF2933 domain-containing protein -
  PSC78_RS12680 (PSC78_12680) - 2588179..2588358 (+) 180 WP_002325573.1 DUF2933 domain-containing protein -
  PSC78_RS12685 (PSC78_12685) - 2588472..2590004 (-) 1533 WP_010730964.1 IS1182 family transposase -
  PSC78_RS12690 (PSC78_12690) - 2590129..2591037 (+) 909 WP_002324242.1 IS982-like element ISEfm1 family transposase -
  PSC78_RS12695 (PSC78_12695) - 2591781..2593064 (-) 1284 WP_002328342.1 hypothetical protein -
  PSC78_RS12700 (PSC78_12700) - 2593243..2593650 (-) 408 WP_002307641.1 NusG domain II-containing protein -
  PSC78_RS12705 (PSC78_12705) - 2593922..2595421 (+) 1500 WP_043902900.1 peptidoglycan DD-metalloendopeptidase family protein -
  PSC78_RS12710 (PSC78_12710) - 2595576..2596112 (+) 537 WP_002307637.1 class A sortase -
  PSC78_RS12715 (PSC78_12715) - 2596434..2596640 (-) 207 WP_002328344.1 hypothetical protein -
  PSC78_RS12720 (PSC78_12720) - 2596650..2596925 (-) 276 WP_002352467.1 hypothetical protein -
  PSC78_RS12725 (PSC78_12725) - 2597028..2597294 (-) 267 WP_002307629.1 YfhO family protein -
  PSC78_RS12730 (PSC78_12730) - 2597488..2598282 (-) 795 Protein_2488 IS3-like element IS1485 family transposase -
  PSC78_RS12735 (PSC78_12735) - 2598297..2599250 (-) 954 WP_126265779.1 IS30 family transposase -
  PSC78_RS12740 (PSC78_12740) - 2599499..2600587 (-) 1089 WP_002286103.1 hypothetical protein -
  PSC78_RS12745 (PSC78_12745) rlmH 2600733..2601212 (-) 480 WP_104681399.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  PSC78_RS12750 (PSC78_12750) htrA 2601613..2602911 (+) 1299 WP_002368406.1 S1C family serine protease Regulator

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 44831.95 Da        Isoelectric Point: 4.9968

>NTDB_id=786120 PSC78_RS12750 WP_002368406.1 2601613..2602911(+) (htrA) [Enterococcus faecalis strain CQJXZ21-077]
MQRKDVTPNSDKKSLLQKFGIGLAGGLLGGALILGGAYSGIIPTPNGGNNAATTTSTNHGDTKVSNVSYNVSSDVTKAVK
KVQNSVVSVINMQSASNNSSADDPFGGLFGGNEGTQDSSGNNGNDLEAASEGSGVIYKKDGKTAYVVTNNHVVDKAQGLE
VVLSDGTKVKGELVGTDAYTDLAVIKISSDKVDQVAEFGNSSKITVGEPAIAIGSPLGSDYANSVTQGIISSVNRNITNK
NESGETININAIQTDAAINPGNSGGPLINIEGQVIGINSVKIVQSTSQVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPA
LGITMSDLTGISSQQQEQILKIPASVKTGVVVRGVEAATPAEKAGLEKYDVITKVDGQDVSSTTDLQSALYKKKVGDKME
VTYYRGSKEMKATIDLTIDKSALTQQNNRSNQ

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=786120 PSC78_RS12750 WP_002368406.1 2601613..2602911(+) (htrA) [Enterococcus faecalis strain CQJXZ21-077]
ATGCAACGAAAAGATGTTACACCTAATTCAGATAAAAAAAGTTTATTGCAAAAATTTGGGATTGGTTTAGCTGGCGGCTT
ACTTGGTGGCGCCCTTATTCTAGGAGGCGCTTATTCTGGGATTATTCCCACACCCAACGGCGGAAATAATGCGGCGACGA
CGACGTCCACTAATCACGGCGACACAAAAGTCAGCAATGTGAGTTACAATGTGTCTAGCGATGTCACAAAAGCCGTGAAA
AAAGTTCAAAATTCTGTGGTTTCTGTCATTAATATGCAAAGTGCTAGCAACAATTCTTCGGCAGATGATCCTTTTGGGGG
ATTGTTCGGTGGGAATGAAGGTACTCAAGACTCTTCCGGCAACAATGGTAACGATTTAGAAGCAGCCAGTGAAGGTAGTG
GGGTTATTTATAAGAAAGATGGTAAAACGGCTTATGTCGTGACAAATAACCACGTCGTAGATAAAGCGCAAGGATTGGAA
GTTGTTTTATCTGATGGTACCAAAGTGAAAGGCGAATTAGTCGGAACCGATGCTTACACGGATTTAGCCGTGATTAAAAT
TTCTTCCGACAAAGTTGATCAAGTCGCTGAGTTTGGGAATTCTAGTAAAATCACAGTCGGTGAGCCTGCTATTGCGATTG
GTTCCCCTCTAGGTTCTGATTATGCTAACTCTGTCACACAAGGAATCATCTCTTCTGTGAATAGAAATATTACCAATAAA
AACGAGTCTGGTGAAACCATCAATATTAATGCCATTCAAACCGATGCTGCCATCAATCCAGGAAACTCCGGTGGTCCACT
AATCAATATTGAAGGACAAGTCATTGGAATTAACTCAGTAAAAATTGTGCAATCAACTAGTCAAGTGAGCGTTGAAGGGA
TGGGCTTTGCCATTCCAAGTAATGACGTAGTCAACATTATCAACCAATTAGAAAAAGATGGTAAAGTGACGCGGCCAGCT
CTCGGGATTACGATGTCTGATTTAACAGGTATCTCTTCACAACAACAAGAACAAATTTTAAAAATTCCAGCTTCTGTAAA
AACTGGCGTAGTGGTTCGTGGCGTTGAAGCAGCGACCCCTGCTGAAAAGGCTGGATTGGAAAAATACGATGTTATCACGA
AAGTTGACGGCCAAGACGTAAGCTCTACTACAGATTTACAAAGCGCGCTTTACAAGAAAAAAGTTGGCGACAAAATGGAA
GTGACTTATTATCGTGGTTCTAAAGAAATGAAAGCAACCATCGATTTAACCATTGATAAATCAGCTTTAACACAACAAAA
TAATCGCAGCAATCAATAA

Domains

Predicted by InterproScan.

Trypsin-like peptidase domain (Trypsin_2)

(132-277)

Peptidase family M50 (Peptidase_M50)

(9-416)

PDZ domain (PDZ_2)

(319-416)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R3K7C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus gordonii str. Challis substr. CH1

53.171

94.907

0.505
  htrA Streptococcus mutans UA159

58.63

84.491

0.495
  htrA Streptococcus mitis NCTC 12261

55.135

85.648

0.472
  htrA Streptococcus pneumoniae TIGR4

55.807

81.713

0.456
  htrA Streptococcus pneumoniae Rx1

55.807

81.713

0.456
  htrA Streptococcus pneumoniae D39

55.807

81.713

0.456
  htrA Streptococcus pneumoniae R6

55.807

81.713

0.456