Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP/pknB   Type   Regulator
Locus tag   LLL8_RS09800 Genome accession   NZ_AP025700
Coordinates   2017147..2019030 (-) Length   627 a.a.
NCBI ID   WP_251899864.1    Uniprot ID   -
Organism   Lactococcus lactis strain L8     
Function   activate comX expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 2012147..2024030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLL8_RS09770 (LLL8_19210) - 2012553..2013233 (-) 681 WP_017864968.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  LLL8_RS09775 (LLL8_19220) macP 2013265..2013543 (-) 279 WP_251899861.1 cell wall synthase accessory phosphoprotein MacP -
  LLL8_RS09780 (LLL8_19230) - 2013536..2014120 (-) 585 WP_251899862.1 NUDIX hydrolase -
  LLL8_RS09785 (LLL8_19240) glmU 2014225..2015601 (-) 1377 WP_003130798.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  LLL8_RS09790 (LLL8_19250) proC 2015829..2016617 (+) 789 WP_251899863.1 pyrroline-5-carboxylate reductase -
  LLL8_RS09795 (LLL8_19260) rpsO 2016696..2016965 (-) 270 WP_015427039.1 30S ribosomal protein S15 -
  LLL8_RS09800 (LLL8_19270) stkP/pknB 2017147..2019030 (-) 1884 WP_251899864.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  LLL8_RS09805 (LLL8_19280) - 2019030..2019806 (-) 777 WP_003130802.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  LLL8_RS09810 (LLL8_19290) rsmB 2019887..2021161 (-) 1275 WP_058203445.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  LLL8_RS09815 (LLL8_19300) - 2021360..2021866 (-) 507 WP_012898444.1 Asp23/Gls24 family envelope stress response protein -
  LLL8_RS09820 (LLL8_19310) - 2021887..2022075 (-) 189 WP_012898445.1 DUF2273 domain-containing protein -
  LLL8_RS09825 (LLL8_19320) amaP 2022086..2022646 (-) 561 WP_010906186.1 alkaline shock response membrane anchor protein AmaP -
  LLL8_RS09830 (LLL8_19330) - 2022674..2022916 (-) 243 WP_003131476.1 GlsB/YeaQ/YmgE family stress response membrane protein -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 67968.04 Da        Isoelectric Point: 4.6993

>NTDB_id=78560 LLL8_RS09800 WP_251899864.1 2017147..2019030(-) (stkP/pknB) [Lactococcus lactis strain L8]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVVNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLDQAKSKIKDAKLKVGTVHKQQSSTI
AEGNVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGNEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKTAGFTNITLDPQATTNQQADGYVYSVYPTVGA
SVDPTQEIVITYSVYTAPSSSSTTSESTPTPETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=78560 LLL8_RS09800 WP_251899864.1 2017147..2019030(-) (stkP/pknB) [Lactococcus lactis strain L8]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAACTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATCAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGACCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGTAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CGCTTGACCAAGCAAAAAGCAAAATTAAAGATGCAAAACTAAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAATGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATTTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAATGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAACTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCTGATGGTTATGTATATTCTGTTTATCCGACTGTTGGAGCG
TCCGTTGATCCAACTCAAGAAATTGTAATCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACACCGACGCCTGAAACTTCGTCAAGCACAACTTCAAGTACTTCAAGTTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGTAGCAAAGAATCTTCGACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(434-501)

PASTA domain (PASTA)

(506-573)

PASTA domain (PASTA)

(365-428)

Protein kinase domain (Pkinase)

(12-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP/pknB Streptococcus salivarius strain HSISS4

46.003

97.767

0.45
  pknB Streptococcus mutans UA159

45.219

98.405

0.445
  stkP Streptococcus pneumoniae TIGR4

48.024

80.702

0.388
  stkP Streptococcus pneumoniae D39

47.826

80.702

0.386
  stkP Streptococcus pneumoniae R6

47.826

80.702

0.386
  stkP Streptococcus pneumoniae Rx1

47.628

80.702

0.384

Multiple sequence alignment