Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH88_RS04780 Genome accession   NZ_CP117430
Coordinates   1010666..1012363 (-) Length   565 a.a.
NCBI ID   WP_305425150.1    Uniprot ID   -
Organism   Pseudomonas wuhanensis strain FP607     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1005666..1017363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH88_RS04745 (PSH88_04745) - 1006142..1006384 (-) 243 WP_305425144.1 DUF2790 domain-containing protein -
  PSH88_RS04750 (PSH88_04750) - 1006777..1007466 (+) 690 WP_305425145.1 energy-coupling factor ABC transporter permease -
  PSH88_RS04755 (PSH88_04755) - 1007530..1007745 (+) 216 WP_007938959.1 hypothetical protein -
  PSH88_RS04760 (PSH88_04760) yacG 1007755..1007955 (-) 201 WP_305425146.1 DNA gyrase inhibitor YacG -
  PSH88_RS04765 (PSH88_04765) coaE 1007952..1008575 (-) 624 WP_305425147.1 dephospho-CoA kinase -
  PSH88_RS04770 (PSH88_04770) pilD 1008572..1009444 (-) 873 WP_305425148.1 A24 family peptidase Machinery gene
  PSH88_RS04775 (PSH88_04775) pilC 1009446..1010663 (-) 1218 WP_305425149.1 type II secretion system F family protein Machinery gene
  PSH88_RS04780 (PSH88_04780) pilB 1010666..1012363 (-) 1698 WP_305425150.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH88_RS04785 (PSH88_04785) - 1012590..1013015 (+) 426 WP_305425151.1 pilin -
  PSH88_RS04790 (PSH88_04790) - 1013333..1013689 (+) 357 WP_305425152.1 BON domain-containing protein -
  PSH88_RS04795 (PSH88_04795) - 1013864..1014160 (-) 297 WP_305425153.1 DUF2845 domain-containing protein -
  PSH88_RS04800 (PSH88_04800) - 1014160..1017147 (-) 2988 WP_305425154.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62884.83 Da        Isoelectric Point: 5.4106

>NTDB_id=784914 PSH88_RS04780 WP_305425150.1 1010666..1012363(-) (pilB) [Pseudomonas wuhanensis strain FP607]
MNDIALSGLAKQLVLAELITDQSAQQAYQQAQRNRISLVSYLVQNKLLKSWQVAEVASEHFGMALLDLNCLDKDTQPRGL
VSEKLIRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLSTEAILVEEDKLSDAIEKFFDNPSTGLEDMADVDLDG
LDIESVDDQHQDSIPGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKIYRVRMRTDGMLREVARPTQLANRIASRLK
VMASLDISERRKPQDGRIKMRLSKSNSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQGM
ILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDLE
TAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPREALLKEGFPEE
RIGSFTIYEPIGCDQCNSGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLLKAMQGITSLEEIN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=784914 PSH88_RS04780 WP_305425150.1 1010666..1012363(-) (pilB) [Pseudomonas wuhanensis strain FP607]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTACTGGCCGAGCTGATCACTGATCAAAGTGCGCAACAGGC
GTATCAACAAGCCCAACGCAATCGAATCTCGCTGGTCAGCTATCTGGTGCAAAACAAACTGCTGAAGAGCTGGCAAGTCG
CCGAGGTGGCCTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCAATTGCCTGGACAAGGACACCCAACCCCGAGGGCTG
GTCAGCGAGAAACTGATCCGCCAGCACCACGCCCTGCCCCTCTGGCGACGGGGTAACAAGCTGTTCGTGGGGATTTCCGA
CCCGACCAATCATCAAGCCATCAACGACATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTGGAGGAAGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGACAACCCCTCCACCGGTCTGGAAGACATGGCCGATGTCGACCTCGACGGG
CTGGACATCGAGTCGGTCGATGACCAGCACCAGGATTCCATCCCGGGGCAAGATACCGATGATGCGCCCGTGGTGCGCTT
CGTCCACAAGATGCTGCTCGACGCGATCAAGAGCGGTTCTTCCGACCTGCATTTCGAGCCCTATGAAAAAATCTACCGCG
TGCGGATGCGCACCGATGGCATGCTGCGAGAGGTCGCAAGGCCGACACAACTGGCCAACCGCATTGCTTCGCGGCTGAAG
GTCATGGCCAGTCTCGACATCTCCGAACGGCGCAAACCCCAGGACGGGCGAATCAAGATGCGCCTGTCCAAAAGCAACTC
CATCGACTTCCGGGTCAACACCCTGCCAACCCTGTGGGGCGAGAAGGTGGTGATCCGGATCCTCGACCCGTCCAGTGCCC
AAATGGGCATCGATGCCCTCGGTTACGAACCCGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCACAAGGGATG
ATTCTGGTGACCGGTCCCACCGGCTCGGGCAAGACCGTGTCGCTCTATACAGGGTTGAACATCCTCAACACCGTCGACAT
CAATATCTCCACCGCCGAAGACCCGGTAGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAGGCAAGGAC
TGGATTTCGCCCAGGCGCTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGCGACCTCGAA
ACCGCCGAAATCGCCATCAAGGCTGCCCAGACCGGGCACATGGTACTTTCCACCCTACACACCAACAGCGCCGCGCAAAC
CCTGACACGCCTGCACAACATGGGGATTCAGGGCTTCAACATCGCGACCTCGGTCAGCCTGATCATCGCTCAGCGCCTGG
CGCGCAAACTGTGCAGTCATTGCAAAAAGCCCATCGAGATTCCCCGCGAGGCGTTGCTCAAAGAAGGCTTTCCCGAGGAA
CGCATCGGCTCGTTCACGATCTATGAACCGATCGGTTGCGATCAGTGCAACAGCGGTTACAAAGGGCGAGTAGGGATTTA
TGAAGTGGTAAAGAACACCCCAGACCTGCAACGGCTGATCATGGCCGAAGGCAACTCACTGGAAATCGACATCCAGATGC
GTAAGGACGGCTTCAACGACCTGCGTACTTCGGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATCAAC
CGGGTCACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.979

99.823

0.579
  pilB Acinetobacter baylyi ADP1

57.597

100

0.577
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.559
  pilB Vibrio cholerae strain A1552

50.796

100

0.508
  pilF Neisseria gonorrhoeae MS11

48.845

99.646

0.487
  pilB Vibrio campbellii strain DS40M4

48.07

100

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.407

0.481
  pilF Thermus thermophilus HB27

36.749

100

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.857

92.92

0.361