Detailed information NTG0782782

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PO847_RS13610
• Genome accession: NZ_CP117197
• Coordinates: 2729646..2730260 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain 3ZT
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2724646..2735260

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PO847_RS13580 (PO847_13580)	rpsT	2724949..2725215 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PO847_RS13585 (PO847_13585)	holA	2725232..2726273 (-)	1042	Protein_2629	DNA polymerase III subunit delta	-
PO847_RS13590 (PO847_13590)	-	2726313..2726464 (-)	152	Protein_2630	hypothetical protein	-
PO847_RS13595 (PO847_13595)	-	2726505..2726639 (+)	135	WP_003152870.1	YqzM family protein	-
PO847_RS13600 (PO847_13600)	comEC	2726658..2729009 (-)	2352	WP_089072447.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PO847_RS13605 (PO847_13605)	-	2729010..2729579 (-)	570	WP_003152868.1	ComE operon protein 2	-
PO847_RS13610 (PO847_13610)	comEA	2729646..2730260 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
PO847_RS13615 (PO847_13615)	comER	2730319..2731140 (+)	822	WP_014418420.1	late competence protein ComER	-
PO847_RS13620 (PO847_13620)	-	2731209..2731946 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
PO847_RS13625 (PO847_13625)	rsfS	2731943..2732299 (-)	357	WP_007408260.1	ribosome silencing factor	-
PO847_RS13630 (PO847_13630)	yqeK	2732317..2732877 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PO847_RS13635 (PO847_13635)	-	2732867..2733436 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
PO847_RS13640 (PO847_13640)	yhbY	2733447..2733737 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PO847_RS13645 (PO847_13645)	aroE	2733731..2734573 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=782782 PO847_RS13610 WP_014418419.1 2729646..2730260(-) (comEA) [Bacillus velezensis strain 3ZT]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=782782 PO847_RS13610 WP_014418419.1 2729646..2730260(-) (comEA) [Bacillus velezensis strain 3ZT]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363