Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PQV96_RS16485 Genome accession   NZ_CP117193
Coordinates   3526387..3528120 (+) Length   577 a.a.
NCBI ID   WP_273623611.1    Uniprot ID   -
Organism   Acidovorax temperans strain LMJ     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 3520626..3525917 3526387..3528120 flank 470


Gene organization within MGE regions


Location: 3520626..3528120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQV96_RS16460 (PQV96_16460) - 3520626..3520889 (-) 264 Protein_3234 transposase -
  PQV96_RS16465 (PQV96_16465) - 3520932..3521975 (+) 1044 Protein_3235 IS630-like element ISStma10 family transposase -
  PQV96_RS16470 (PQV96_16470) - 3522205..3523176 (-) 972 WP_273623609.1 IS481 family transposase -
  PQV96_RS16475 (PQV96_16475) - 3523339..3524667 (-) 1329 WP_207375710.1 IS4 family transposase -
  PQV96_RS16480 (PQV96_16480) - 3524886..3526002 (+) 1117 Protein_3238 IS5 family transposase -
  PQV96_RS16485 (PQV96_16485) pilB 3526387..3528120 (+) 1734 WP_273623611.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62961.18 Da        Isoelectric Point: 5.2505

>NTDB_id=782700 PQV96_RS16485 WP_273623611.1 3526387..3528120(+) (pilB) [Acidovorax temperans strain LMJ]
MATADIAPKDASAIALPGLGRALMSTGKLTQKAAEEIYQKSQISRTSFIAELTGSGVVSAADLAHTVSAVFGAPLLDLEA
IDPLRLPKDLLDAKICQAYKVVVLSKRSNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLNRLVEATTKSASESM
ESLTSGDFEFDESVAEETPEAIDSGANEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELKEISSPPI
AIKDKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPSCKAPADIPH
ETLLEAGYREEDLDGSWLTYRAVGCSACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAEQARNEGVRSLRESGLHK
ARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=782700 PQV96_RS16485 WP_273623611.1 3526387..3528120(+) (pilB) [Acidovorax temperans strain LMJ]
ATGGCTACCGCTGATATTGCCCCCAAAGACGCTTCTGCAATCGCTCTTCCCGGCCTAGGAAGGGCTTTAATGTCTACGGG
CAAGCTTACGCAGAAAGCAGCGGAGGAAATTTACCAAAAGTCACAAATTAGCCGAACAAGCTTTATCGCCGAACTGACGG
GGTCGGGGGTGGTGTCGGCAGCTGACTTGGCACATACGGTTTCTGCAGTTTTTGGAGCACCACTACTTGATTTGGAAGCT
ATTGACCCGCTGCGATTACCGAAAGATTTATTGGATGCCAAGATCTGCCAAGCCTACAAAGTCGTAGTACTGAGCAAACG
TAGCAACCGCTTGATTGTTGCGACGGCGGACCCCACAGACCAAGAGGCAGCAGAAAAAATCAAGTTCACCACCCAGATGG
GCGTGGATTGGATTATCGCGGAATATGACAAACTGAATCGCCTAGTGGAGGCTACAACCAAGTCCGCTTCCGAGTCCATG
GAAAGCCTGACCAGTGGCGATTTTGAATTTGACGAATCAGTTGCAGAGGAAACTCCTGAGGCTATAGATTCAGGAGCCAA
CGAGGTTGAAGATGCTCCCGTTGTTAAGTTTCTTCATAAGATGCTTTTGGATGCTTTTAATATGCGGGCATCTGATTTGC
ATTTTGAACCATACGAACATCAATACCGAGTGCGTTTTCGCATTGACGGCGAGCTGAAAGAAATTTCATCTCCTCCTATC
GCAATTAAAGACAAGCTAGCTTCACGCATTAAAGTGATATCCAGAATGGATATTTCAGAAAAGCGAGTCCCGCAAGATGG
ACGAATGAAGCTCAAGGTAGGTCCAGATCGGGTTATTGACTTTCGAGTGAGTACGCTGCCGACTTTGTTTGGTGAAAAAA
TAGTAATCCGCATTCTTGATCCGAGTAGCGCAAAGCTTGGCATTGATGCTCTGGGATATGAACCAGAAGAGAAAGAGCGA
CTATTACACGCAATTGGTCGGCCATATGGCATGATTCTTGTAACGGGCCCTACAGGCTCAGGAAAAACCGTATCGTTGTA
TACCTGTCTAAATCTATTAAACAAACCAGGCGTAAATATTGCCACCGCGGAAGACCCTTCCGAAATCAATCTACCTGGCG
TTAACCAAGTTAATGTCAACGAAAAGGCCGGACTTACGTTTGCCGTCGCACTGAAATCATTTTTACGCCAAGATCCCGAC
ATCATCATGGTGGGGGAAATTCGAGACCTTGAGACAGCTGATATTTCCATCAAAGCGGCACAAACGGGTCACTTGGTTCT
ATCTACACTCCACACCAATGATGCCCCAACCACACTGACACGCATGCGGAACATGGGTATTGCCCCATTTAATATCGCAT
CCAGTGTGATTCTGATCACAGCTCAGCGCCTGGCGCGACGCTTATGCCCAAGCTGCAAAGCTCCTGCTGATATTCCTCAC
GAGACACTTTTGGAAGCAGGATACCGCGAAGAGGATTTGGATGGATCCTGGCTGACATACAGAGCTGTCGGATGCTCAGC
CTGCAATAATGGTTACAAAGGGCGTGTGGGGATCTACCAAGTCATGCCAATTTCCGAGGAAATTCAGCGCATTATTCTGC
GTGATGGCAGCGCACTCGAAATCGCAGAACAAGCCCGGAACGAGGGGGTGCGCTCCTTGCGAGAGTCCGGCTTGCACAAG
GCTCGGCTTGGGCTCACTTCCCTCGAAGAAGTGCTTGCCGTGACTAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.367

97.227

0.529
  pilF Neisseria gonorrhoeae MS11

53.546

97.747

0.523
  pilB Acinetobacter baumannii D1279779

53.298

97.227

0.518
  pilB Legionella pneumophila strain ERS1305867

49.823

98.094

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

45.487

97.92

0.445
  pilB Vibrio campbellii strain DS40M4

44.974

98.267

0.442
  pilB Vibrio cholerae strain A1552

46.593

94.107

0.438
  pilF Thermus thermophilus HB27

40.105

98.96

0.397
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

97.92

0.39