NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	PO767_RS13425	Genome accession	NZ_CP117182
Coordinates	2700071..2700685 (-)	Length	204 a.a.
NCBI ID	WP_014418419.1	Uniprot ID	I2C7Y9
Organism	Bacillus velezensis strain Lwp6
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2695071..2705685

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PO767_RS13395	rpsT	2695374..2695640 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PO767_RS13400	holA	2695657..2696698 (-)	1042	Protein_2593	DNA polymerase III subunit delta	-
PO767_RS13405	-	2696738..2696889 (-)	152	Protein_2594	hypothetical protein	-
PO767_RS13410	-	2696930..2697064 (+)	135	WP_003152870.1	YqzM family protein	-
PO767_RS13415	comEC	2697083..2699434 (-)	2352	WP_089072447.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PO767_RS13420	-	2699435..2700004 (-)	570	WP_003152868.1	ComE operon protein 2	-
PO767_RS13425	comEA	2700071..2700685 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
PO767_RS13430	comER	2700744..2701565 (+)	822	WP_014418420.1	late competence protein ComER	-
PO767_RS13435	-	2701634..2702371 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
PO767_RS13440	rsfS	2702368..2702724 (-)	357	WP_007408260.1	ribosome silencing factor	-
PO767_RS13445	yqeK	2702742..2703302 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PO767_RS13450	-	2703292..2703861 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
PO767_RS13455	yhbY	2703872..2704162 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PO767_RS13460	aroE	2704156..2704998 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22226.26 Da Isoelectric Point: 5.0355

                                >NTDB_id=782616 PO767_RS13425 WP_014418419.1 2700071..2700685(-) (comEA) [Bacillus velezensis strain Lwp6]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=782616 PO767_RS13425 WP_014418419.1 2700071..2700685(-) (comEA) [Bacillus velezensis strain Lwp6]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	I2C7Y9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363

Detailed information NTG0782616