Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   POF58_RS01130 Genome accession   NZ_CP116711
Coordinates   190164..191207 (+) Length   347 a.a.
NCBI ID   WP_228313184.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 185164..196207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF58_RS01120 (POF58_01120) - 188762..189127 (+) 366 WP_000285373.1 DUF1033 family protein -
  POF58_RS01125 (POF58_01125) comYA 189299..190270 (+) 972 WP_000250801.1 competence type IV pilus ATPase ComGA Machinery gene
  POF58_RS01130 (POF58_01130) comYB 190164..191207 (+) 1044 WP_228313184.1 competence type IV pilus assembly protein ComGB Machinery gene
  POF58_RS01135 (POF58_01135) comGC/cglC 191204..191533 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  POF58_RS01140 (POF58_01140) comYD 191508..191921 (+) 414 WP_000244259.1 competence type IV pilus minor pilin ComGD Machinery gene
  POF58_RS01145 (POF58_01145) comGE 191893..192192 (+) 300 WP_038406052.1 competence type IV pilus minor pilin ComGE -
  POF58_RS01150 (POF58_01150) comGF 192146..192607 (+) 462 WP_001874060.1 competence type IV pilus minor pilin ComGF -
  POF58_RS01155 (POF58_01155) comGG 192585..192956 (+) 372 WP_000601105.1 competence type IV pilus minor pilin ComGG -
  POF58_RS01160 (POF58_01160) comYH 193071..194045 (+) 975 WP_001008567.1 class I SAM-dependent methyltransferase Machinery gene
  POF58_RS01165 (POF58_01165) - 194077..195270 (+) 1194 WP_000047534.1 acetate kinase -
  POF58_RS01170 (POF58_01170) - 195398..195590 (+) 193 Protein_174 helix-turn-helix transcriptional regulator -
  POF58_RS01175 (POF58_01175) - 195569..195835 (+) 267 Protein_175 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 40013.83 Da        Isoelectric Point: 10.0730

>NTDB_id=779455 POF58_RS01130 WP_228313184.1 190164..191207(+) (comYB) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLTFLVLIMIGLRHYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIVQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQN
QKSKLFREIGYDMKEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=779455 POF58_RS01130 WP_228313184.1 190164..191207(+) (comYB) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGATATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGGATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCGTTGATATTAT
TGACTTTTCTGGTGCTAATTATGATTGGTCTTAGGCATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTGTTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTGTTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGAAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGTCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAATTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

61.877

98.271

0.608
  comYB Streptococcus mutans UA159

61.584

98.271

0.605
  comYB Streptococcus gordonii str. Challis substr. CH1

56.305

98.271

0.553
  comGB/cglB Streptococcus mitis NCTC 12261

54.925

96.542

0.53
  comGB/cglB Streptococcus mitis SK321

54.328

96.542

0.524
  comGB/cglB Streptococcus pneumoniae Rx1

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae D39

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae R6

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

53.433

96.542

0.516
  comGB Lactococcus lactis subsp. cremoris KW2

49.852

97.118

0.484