Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   QYH60_RS03125 Genome accession   NZ_CP129292
Coordinates   614468..615469 (+) Length   333 a.a.
NCBI ID   WP_003131994.1    Uniprot ID   Q9CDL3
Organism   Lactococcus lactis subsp. lactis strain KMGR2-43     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 609468..620469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYH60_RS03110 (QYH60_03110) - 610841..611536 (+) 696 WP_301401281.1 hypothetical protein -
  QYH60_RS03115 (QYH60_03115) hexB 611665..613635 (+) 1971 WP_301401282.1 DNA mismatch repair endonuclease MutL Machinery gene
  QYH60_RS03120 (QYH60_03120) ruvA 613748..614341 (+) 594 WP_003131995.1 Holliday junction branch migration protein RuvA Machinery gene
  QYH60_RS03125 (QYH60_03125) ruvB 614468..615469 (+) 1002 WP_003131994.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  QYH60_RS03130 (QYH60_03130) - 615606..616490 (+) 885 WP_012898698.1 XRE/MutR family transcriptional regulator -
  QYH60_RS03135 (QYH60_03135) - 616710..617000 (+) 291 WP_058208533.1 hypothetical protein -
  QYH60_RS03140 (QYH60_03140) - 617067..618251 (+) 1185 WP_301401283.1 ABC transporter permease -
  QYH60_RS03145 (QYH60_03145) - 618248..618865 (+) 618 WP_058208535.1 ABC transporter ATP-binding protein -
  QYH60_RS03150 (QYH60_03150) - 618970..619134 (+) 165 WP_003131988.1 hypothetical protein -
  QYH60_RS03155 (QYH60_03155) - 619278..619715 (+) 438 WP_003131986.1 low molecular weight protein-tyrosine-phosphatase -
  QYH60_RS03160 (QYH60_03160) - 619816..620181 (+) 366 WP_003131985.1 MerR family transcriptional regulator -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37633.17 Da        Isoelectric Point: 4.5825

>NTDB_id=778154 QYH60_RS03125 WP_003131994.1 614468..615469(+) (ruvB) [Lactococcus lactis subsp. lactis strain KMGR2-43]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALSILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEED

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=778154 QYH60_RS03125 WP_003131994.1 614468..615469(+) (ruvB) [Lactococcus lactis subsp. lactis strain KMGR2-43]
ATGAACGATATATTAAACAAAGAGCCAATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCA
ATATATTGGTCAAGATAAAGTCAAAGAACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGACC
ACGTGTTACTTTTTGGCCCTCCGGGACTTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGGGTTAATATT
AAACAAACAGCGGGTCCTGCCATTGAAAAGCCAGGAGATTTAGTTGCGATTTTGAATGAATTGGAACCTGGCGATGTACT
ATTTATTGATGAAATTCATCGGATGCCAATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATA
TTATGCTTGGTTCTGGTGATGGTTCACGCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGA
GCTGGAATGCTTTCGAATCCATTGCGTGCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTAGA
AGAAATTGTTAAAAGAACAGCTGATATTTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTC
GAGGGACTCCGCGGATTGCCAATCGTTTACTTAAACGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGAT
AAAGCAATTACCGATAAGGCCTTGTCTATTCTTGATGTTGATGCCGCTGGTTTGGATTATATTGACCAAAAAATTCTCCG
CACAATGATTGAAATGTACCACGGTGGTCCTGTTGGGATTGGAACTTTGGCGGTAAATATTGCCGAAGACCGTGAAACGG
TCGAGGATATGTATGAACCTTATTTGATTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAGGTGACCCAAAGA
GCCTATGAACACTTAGGTTACGTTTATAATGAAGAAGATTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae D39

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae TIGR4

78.313

99.7

0.781
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.491

96.697

0.595
  ruvB Helicobacter pylori 26695

51.242

96.697

0.495
  ruvB Synechocystis sp. PCC 6803

51.613

93.093

0.48