Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PJU76_RS11900 Genome accession   NZ_CP116458
Coordinates   2370248..2371957 (-) Length   569 a.a.
NCBI ID   WP_215849997.1    Uniprot ID   -
Organism   Acidithiobacillus ferriphilus strain YL25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2365248..2376957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJU76_RS11880 (PJU76_11880) coaE 2365769..2366371 (-) 603 WP_215861727.1 dephospho-CoA kinase -
  PJU76_RS11885 (PJU76_11885) - 2366372..2367172 (-) 801 WP_271779692.1 A24 family peptidase -
  PJU76_RS11890 (PJU76_11890) pilR 2367169..2368527 (-) 1359 WP_271779693.1 sigma-54 dependent transcriptional regulator Regulator
  PJU76_RS11895 (PJU76_11895) - 2368524..2370122 (-) 1599 WP_271779694.1 nitrogen regulation protein NR(II) -
  PJU76_RS11900 (PJU76_11900) pilB 2370248..2371957 (-) 1710 WP_215849997.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PJU76_RS11905 (PJU76_11905) aroE 2371927..2372826 (-) 900 WP_271779695.1 shikimate dehydrogenase -
  PJU76_RS11910 (PJU76_11910) - 2372804..2373967 (-) 1164 WP_271779696.1 anhydro-N-acetylmuramic acid kinase -
  PJU76_RS11915 (PJU76_11915) - 2374035..2374523 (-) 489 WP_064218850.1 peptidylprolyl isomerase -
  PJU76_RS11920 (PJU76_11920) hemH 2374575..2375597 (-) 1023 WP_271779697.1 ferrochelatase -
  PJU76_RS11925 (PJU76_11925) xth 2375594..2376367 (-) 774 WP_215850000.1 exodeoxyribonuclease III -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62827.54 Da        Isoelectric Point: 6.3004

>NTDB_id=777479 PJU76_RS11900 WP_215849997.1 2370248..2371957(-) (pilB) [Acidithiobacillus ferriphilus strain YL25]
MATNPAHITLTPVLRALVSNGLSDEAQLQSLAADPARGKMPLLFYAVERGAVPASVLMAHLSARYNMPMLDLDAVMINEL
LIRKLDKGLMTRYLVLPLSKHGDTLYLAMADPTDFKAVEDVKFNTGLQVLPILVEANKLVKAVNAAANSMQGGIDDVFMD
KPRGEEEQEEFDLAQESDGSVEDAPVVRFARQLLLDAIQRGVSDIHLEPYEKDFRVRYRLDGVLQDVLHPPVALRDGVTS
RLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETIVLRLLDPASSKVPIEQLGFLPDQRKAFEEAIHRP
YGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLNFATALRSFLRQDPDIIMVGEVR
DLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGGVHLVMAQRLARKLCPHCKKPLRIPEQALIEAGF
THEDLQGWRPLGAVGCDQCNNTGYKGRMGLYQVMPVSDAMREIIMRGGTSIDLARQATQEGVLTMRQNGLRRVREGVTSL
EEVLRVTNL

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=777479 PJU76_RS11900 WP_215849997.1 2370248..2371957(-) (pilB) [Acidithiobacillus ferriphilus strain YL25]
ATGGCTACAAATCCAGCACACATCACGTTGACGCCCGTATTGCGGGCGTTGGTCAGCAACGGGTTAAGCGATGAGGCGCA
GTTGCAAAGCCTTGCCGCCGATCCGGCGCGTGGCAAGATGCCGCTGCTGTTTTATGCGGTTGAGAGGGGTGCCGTTCCTG
CCTCGGTGCTTATGGCCCACCTTTCCGCGCGCTATAACATGCCCATGCTTGATCTGGATGCGGTCATGATTAACGAGTTG
CTTATTCGGAAGCTCGACAAGGGACTGATGACCCGCTATCTGGTGCTGCCCTTGTCGAAGCATGGGGACACCCTCTACCT
CGCCATGGCGGACCCTACGGATTTTAAGGCGGTGGAGGATGTGAAGTTCAACACGGGTCTGCAGGTGTTGCCAATACTGG
TCGAGGCCAATAAGCTGGTAAAGGCGGTGAATGCCGCAGCCAACAGTATGCAAGGCGGGATTGACGATGTTTTCATGGAT
AAACCGCGCGGCGAAGAAGAGCAGGAAGAATTCGATCTTGCCCAGGAGAGTGATGGCAGTGTCGAGGATGCACCCGTGGT
GCGTTTTGCCCGGCAATTGCTCTTGGATGCCATTCAACGCGGGGTTTCCGACATTCATCTAGAACCGTACGAAAAGGACT
TTCGGGTGCGTTACCGACTCGATGGCGTATTGCAGGATGTGTTGCATCCGCCGGTGGCGTTGCGTGACGGTGTCACTTCG
CGCCTGAAGATTCTCTGTCGTCTGGATATTTCTGAGCGGCGTTTACCTCAGGATGGCCGTTTGCGGGTCAGGGTGCCGCC
GGCGCGGGTTATTGATTTCCGGGTTTCTTTTTTGCCCACCAGTTTTGGCGAAACCATTGTGCTGCGCTTGCTGGATCCGG
CCAGTTCCAAGGTGCCCATAGAACAACTGGGCTTTTTGCCGGACCAGCGCAAAGCTTTTGAAGAGGCCATCCACCGGCCC
TACGGTATGATCCTGGTCACCGGCCCCACGGGTTCGGGTAAAACGACGACTCTTTACACCGCGCTGAATATTCTCAATAC
CGGCGACTGCAACATCAGTACCGCAGAGGATCCTGTGGAAATCCCGGTGTACGGGATTAATCAGGTGAATATCAACGAGC
GTATCGGTCTCAACTTTGCCACCGCCTTGCGTTCCTTTCTGCGTCAGGACCCTGACATCATTATGGTCGGTGAGGTGCGT
GATCTGGAAACCGCGGAAACGGCCATCAAAGCCGCCCAGACGGGTCATCTGGTGTTGGCCACCCTGCATACCAATGATGC
GCCACAGAGCCTCACCCGTCTGGAAAATATGGGCATCCCTACCTATAACATAGCGGGCGGTGTGCACTTGGTGATGGCGC
AGCGACTCGCACGCAAACTCTGTCCGCATTGTAAAAAGCCGCTGCGCATTCCCGAACAGGCCCTGATTGAGGCGGGATTT
ACGCACGAGGATCTGCAGGGTTGGCGTCCCCTGGGCGCGGTGGGCTGTGATCAGTGCAATAACACCGGATATAAAGGCCG
GATGGGACTTTATCAGGTGATGCCGGTGAGCGATGCTATGCGTGAAATCATTATGCGCGGTGGTACCTCCATCGATCTGG
CGCGGCAGGCGACACAAGAGGGAGTGCTGACCATGCGGCAAAACGGATTGAGGCGGGTCAGGGAGGGTGTCACCAGTCTT
GAAGAGGTCCTGCGGGTGACCAATCTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-146)

Type II/IV secretion system protein (T2SSE)

(193-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

48.354

100

0.49
  pilB Acinetobacter baumannii D1279779

48.063

99.824

0.48
  pilB Acinetobacter baylyi ADP1

46.479

99.824

0.464
  pilF Neisseria gonorrhoeae MS11

45.276

98.594

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

43.281

100

0.436
  pilB Vibrio cholerae strain A1552

46.641

91.564

0.427
  pilB Vibrio campbellii strain DS40M4

44.828

91.74

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

93.322

0.371