Detailed information NTG0774951

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PF975_RS07435
• Genome accession: NZ_CP116010
• Coordinates: 1465798..1466412 (+)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain SRCM123815
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1460798..1471412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF975_RS07400 (PF975_07400)	aroE	1461485..1462327 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
PF975_RS07405 (PF975_07405)	yhbY	1462321..1462611 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PF975_RS07410 (PF975_07410)	-	1462622..1463191 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
PF975_RS07415 (PF975_07415)	yqeK	1463181..1463741 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PF975_RS07420 (PF975_07420)	rsfS	1463759..1464115 (+)	357	WP_007408260.1	ribosome silencing factor	-
PF975_RS07425 (PF975_07425)	-	1464112..1464849 (+)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
PF975_RS07430 (PF975_07430)	comER	1464918..1465739 (-)	822	WP_088056446.1	late competence protein ComER	-
PF975_RS07435 (PF975_07435)	comEA	1465798..1466412 (+)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
PF975_RS07440 (PF975_07440)	-	1466479..1467048 (+)	570	WP_003152868.1	ComE operon protein 2	-
PF975_RS07445 (PF975_07445)	comEC	1467049..1469400 (+)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PF975_RS07450 (PF975_07450)	-	1469419..1469553 (-)	135	WP_003152870.1	YqzM family protein	-
PF975_RS07455 (PF975_07455)	-	1469594..1469745 (+)	152	Protein_1458	hypothetical protein	-
PF975_RS07460 (PF975_07460)	holA	1469785..1470826 (+)	1042	Protein_1459	DNA polymerase III subunit delta	-
PF975_RS07465 (PF975_07465)	rpsT	1470843..1471109 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=774951 PF975_RS07435 WP_014418419.1 1465798..1466412(+) (comEA) [Bacillus velezensis strain SRCM123815]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=774951 PF975_RS07435 WP_014418419.1 1465798..1466412(+) (comEA) [Bacillus velezensis strain SRCM123815]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363