Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PE066_RS12975 Genome accession   NZ_CP116009
Coordinates   2733276..2734412 (+) Length   378 a.a.
NCBI ID   WP_271232956.1    Uniprot ID   -
Organism   Ramlibacter tataouinensis strain DMF-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2728276..2739412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE066_RS12955 (PE066_12955) glpK 2729247..2730740 (+) 1494 WP_271232952.1 glycerol kinase GlpK -
  PE066_RS12960 (PE066_12960) - 2730793..2731341 (+) 549 WP_271232953.1 DUF3617 domain-containing protein -
  PE066_RS12965 (PE066_12965) - 2731446..2732138 (-) 693 WP_271232954.1 YggS family pyridoxal phosphate-dependent enzyme -
  PE066_RS12970 (PE066_12970) pilT 2732187..2733230 (+) 1044 WP_271232955.1 type IV pilus twitching motility protein PilT Machinery gene
  PE066_RS12975 (PE066_12975) pilU 2733276..2734412 (+) 1137 WP_271232956.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PE066_RS12980 (PE066_12980) - 2734642..2735544 (+) 903 WP_271232957.1 NAD(P)-dependent oxidoreductase -
  PE066_RS12985 (PE066_12985) - 2735691..2736332 (-) 642 WP_271232958.1 BON domain-containing protein -
  PE066_RS12990 (PE066_12990) - 2736329..2736928 (-) 600 WP_271232959.1 phosphoheptose isomerase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42039.40 Da        Isoelectric Point: 7.1984

>NTDB_id=774918 PE066_RS12975 WP_271232956.1 2733276..2734412(+) (pilU) [Ramlibacter tataouinensis strain DMF-7]
MERDQASKFINDLLKLMISRNGSDLFITGDFPPAIKVDGKVTKVSPQPLTGQHTLALARSIMNDKQASEFERTKECNFAI
SPPGIGRFRVNAFVQQGKVGMVLRVIPQSLPTIDKLGVPQVLKEVTMAKRGLCILVGATGSGKSTTLAAMIDWRNENSWG
HIITIEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFAETGHLCMATLHANSA
NQALDRVINFFPEERRAQLLMDLSLNLKAMVSQRLVPKQDGKGRHAAVEIMLNTPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQALFDAYENNIISYEDALRNADSVNDLRLQIKLNSQRAKTQDLSSGTEHLAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=774918 PE066_RS12975 WP_271232956.1 2733276..2734412(+) (pilU) [Ramlibacter tataouinensis strain DMF-7]
ATGGAACGCGACCAGGCCAGCAAATTCATCAACGACCTGCTCAAGCTGATGATCAGCCGCAACGGCAGCGACCTGTTCAT
CACCGGCGACTTCCCGCCCGCCATCAAGGTGGACGGCAAGGTCACCAAGGTGTCGCCGCAGCCGCTGACCGGCCAGCACA
CGCTGGCGCTGGCGCGCTCGATCATGAACGACAAGCAGGCGTCGGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCGCCGCCGGGCATCGGGCGCTTCCGGGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGGTGATTCC
GCAGAGCCTGCCGACCATCGACAAGCTGGGCGTGCCGCAGGTGCTCAAGGAAGTGACGATGGCCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTGGCGCAACGAGAACTCCTGGGGC
CACATCATCACCATCGAGGACCCGGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAGGTCGGGCT
GGACACCGACAGCTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCACCGGACGTCATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGAGCATGCCATCGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACACTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGGGTGATCAACTTCTTCCCCGAGGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTCAACCT
CAAGGCCATGGTGTCGCAGCGGCTGGTGCCCAAGCAGGACGGCAAGGGCCGGCACGCGGCGGTGGAGATCATGCTCAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCTCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACGCCTACGAGAACAACATCATTTCGTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGGCTGCAGATCAAGCTCAACTCGCAGCGCGCCAAGACCCAGGACCTGTCCTCGGGCACGGAAC
ACCTGGCGATTGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

58.496

94.974

0.556
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Legionella pneumophila strain ERS1305867

43.544

88.095

0.384
  pilT Legionella pneumophila strain Lp02

43.544

88.095

0.384
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

41.441

88.095

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362