Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PE064_RS10735 Genome accession   NZ_CP116007
Coordinates   2303725..2305467 (-) Length   580 a.a.
NCBI ID   WP_271249195.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain DMF-5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2298725..2310467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE064_RS10715 (PE064_10715) - 2299507..2299932 (+) 426 WP_271249192.1 transposase -
  PE064_RS10720 (PE064_10720) coaE 2300721..2301338 (-) 618 WP_271249193.1 dephospho-CoA kinase -
  PE064_RS10725 (PE064_10725) pilD 2301372..2302235 (-) 864 WP_187573334.1 A24 family peptidase Machinery gene
  PE064_RS10730 (PE064_10730) pilC 2302245..2303501 (-) 1257 WP_271249194.1 type II secretion system F family protein Machinery gene
  PE064_RS10735 (PE064_10735) pilB 2303725..2305467 (-) 1743 WP_271249195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PE064_RS10740 (PE064_10740) - 2305618..2306544 (-) 927 WP_271249196.1 hypothetical protein -
  PE064_RS10745 (PE064_10745) - 2306514..2307122 (-) 609 WP_271249197.1 class I SAM-dependent methyltransferase -
  PE064_RS10750 (PE064_10750) - 2307119..2308093 (-) 975 WP_271249198.1 glycosyltransferase family 2 protein -
  PE064_RS10755 (PE064_10755) - 2308090..2310135 (-) 2046 WP_271249199.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62732.16 Da        Isoelectric Point: 6.3331

>NTDB_id=774880 PE064_RS10735 WP_271249195.1 2303725..2305467(-) (pilB) [Pseudoxanthomonas mexicana strain DMF-5]
MNAVASPTANLVGITGIARRLVQDGAMDEATARSAMANATEAKVPLAQWVAEKRLVAPAHLAAANAIEFGMPLIDVTAFD
PAHHAVKLVSEELIQKHQVLPLFKRGGKLFVGTSNPTQTAALTEIQFHTNLVVEPVLVDDDQLRRTLDQWLNAASNLSGS
FGGDADEGLDNLEVGTDEDMGGGADSAVDAKGDDTPVVKFVNKVLVDAIKRGASDIHFEPYETDYRVRLRIDGILKQVAK
APVKLSSRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPDQ
QKLFEEAIQKPYGMVLVTGPTGSGKTVSLYTALGILNDEVRNISTVEDPVEIRLPGVNQVQQNTKRGMTFAAALRSFLRQ
DPDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPKCKKPVE
LPKPALLAEGFTEAEIDAGFTLYDAVGCDECTEGYKGRTGIYQVMPMSDDIQAIVLEGGNAMQIAAVARKAGIRDLRQSA
LMKVRNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=774880 PE064_RS10735 WP_271249195.1 2303725..2305467(-) (pilB) [Pseudoxanthomonas mexicana strain DMF-5]
ATGAATGCAGTCGCCAGCCCCACCGCCAATCTCGTCGGCATTACCGGCATTGCCCGCCGCCTGGTTCAGGATGGCGCGAT
GGATGAAGCCACTGCCCGCAGCGCGATGGCCAACGCCACGGAGGCCAAGGTGCCCTTGGCGCAGTGGGTGGCCGAGAAGC
GCCTCGTCGCGCCCGCCCACCTGGCCGCCGCCAACGCCATCGAATTCGGCATGCCGCTGATCGATGTCACCGCGTTCGAC
CCCGCCCACCACGCCGTCAAGCTGGTCAGCGAGGAACTGATCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCGG
CAAGCTGTTCGTCGGCACCAGCAATCCCACCCAGACCGCGGCCCTGACCGAAATCCAGTTCCACACCAACCTGGTGGTGG
AGCCCGTGCTGGTCGATGATGACCAGCTGCGTCGCACCCTGGACCAGTGGCTCAACGCCGCCAGCAACCTGTCCGGCAGC
TTCGGCGGCGACGCCGACGAGGGGTTGGACAACCTAGAGGTGGGTACTGACGAGGACATGGGCGGTGGCGCCGACAGCGC
CGTCGACGCCAAGGGCGACGACACGCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTAGACGCCATCAAGCGCGGCGCTT
CGGACATCCACTTCGAACCCTACGAGACCGACTACCGCGTACGCCTGCGCATCGACGGCATCCTCAAGCAGGTGGCCAAG
GCGCCGGTGAAGCTGAGCTCGCGCATCGCCGCGCGCCTGAAGGTGATGTCGCAGTTGGACATCGCCGAGAAGCGCGTGCC
GCAGGACGGCCGCATCAAGCTGAACCTATCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCG
GCGAGAAGATCGTGCTGCGCATCTTGGACGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAG
CAGAAGCTGTTCGAAGAGGCCATCCAGAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGT
GTCGCTGTACACGGCGCTGGGCATCCTGAACGACGAGGTCCGCAACATCTCCACTGTCGAGGACCCGGTGGAAATCCGCC
TGCCCGGCGTGAACCAGGTGCAGCAGAACACCAAACGCGGCATGACCTTCGCCGCGGCCCTGCGCAGCTTCCTGCGCCAG
GACCCCGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCA
CATGGTGCTGTCCACCCTGCACACCAACGACGCTCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCCCCGTACA
ACATCACCAGCTCGGTGACCCTGGTGATCGCCCAGCGCCTGGCGCGGCGGCTGTGCCCCAAGTGCAAGAAACCGGTCGAA
CTGCCCAAGCCCGCCCTGCTGGCCGAAGGCTTCACCGAGGCCGAGATCGACGCCGGCTTCACCCTGTACGACGCGGTCGG
CTGCGACGAGTGCACCGAGGGCTACAAGGGCCGGACCGGCATCTACCAGGTCATGCCGATGTCCGACGACATCCAGGCCA
TCGTGCTGGAAGGCGGCAACGCCATGCAGATCGCCGCCGTCGCCCGGAAGGCCGGCATCCGCGACCTGCGCCAGTCCGCG
CTGATGAAGGTCCGCAACGGCGTCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.491

98.276

0.555
  pilB Acinetobacter baylyi ADP1

54.733

100

0.548
  pilB Legionella pneumophila strain ERS1305867

51.761

97.931

0.507
  pilF Neisseria gonorrhoeae MS11

49.649

98.276

0.488
  pilB Vibrio cholerae strain A1552

52.317

89.31

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

46.384

97.759

0.453
  pilB Vibrio campbellii strain DS40M4

45.439

98.276

0.447