Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PE074_RS00665 Genome accession   NZ_CP115969
Coordinates   138275..139966 (-) Length   563 a.a.
NCBI ID   WP_008315358.1    Uniprot ID   A0AB35BVX0
Organism   Wohlfahrtiimonas chitiniclastica strain BM-Y     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 133275..144966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE074_RS00645 (PE074_00645) pckA 134464..136077 (+) 1614 WP_008315353.1 phosphoenolpyruvate carboxykinase (ATP) -
  PE074_RS00650 (PE074_00650) - 136128..137165 (-) 1038 WP_063455244.1 methyltransferase -
  PE074_RS00655 (PE074_00655) - 137162..137851 (-) 690 WP_063455245.1 pseudouridine synthase -
  PE074_RS00660 (PE074_00660) - 137855..138235 (-) 381 WP_082818395.1 RidA family protein -
  PE074_RS00665 (PE074_00665) pilB 138275..139966 (-) 1692 WP_008315358.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PE074_RS00670 (PE074_00670) - 140168..141130 (+) 963 WP_008315359.1 PhoH family protein -
  PE074_RS00675 (PE074_00675) ybeY 141123..141590 (+) 468 WP_008315364.1 rRNA maturation RNase YbeY -
  PE074_RS00680 (PE074_00680) putP 141619..143181 (+) 1563 WP_063455247.1 sodium/proline symporter PutP -
  PE074_RS00685 (PE074_00685) - 143230..144237 (+) 1008 WP_063455248.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62638.55 Da        Isoelectric Point: 6.1457

>NTDB_id=774705 PE074_RS00665 WP_008315358.1 138275..139966(-) (pilB) [Wohlfahrtiimonas chitiniclastica strain BM-Y]
MRLMHNGLVKALMEKGLVTEAHLDRADTIMQADKCSLMRALITLNIAPQALFEVTQSLSNLPRFDFRAFDLRTKPPELLS
TEEMEKNLVFPLRMKNGTLYVAVADPTDSRMLETLRFKSRAIIEPILVAPMQLVAHFESYGGVNREKLQSIFTQMETQKK
EETTDPDASVTYNLMSDKDNSPIVRFINGVLADAITRGVSDLHFEPYEHSYRVRFRIDGVLQEVYTPPDSFRDQIAARIK
VMSQLDITEKRVPQDGRIKVDLKGRIIDFRVSICPTLWGEKIVMRILDSSAANLNIEILGFSDIQKKNILEAIQKPQGMI
LVTGPTGSGKTVTLYTCLGILNQPTTNISTAEDPVEINLEGINQVPVNPKVGLNFAAALKSFLRQDPDIIMVGEIRDLET
ADIAIKAAQTGHLVLSTLHTNSAPETLTRLINMGVETFNIASTVHLIIAQRLARRLCSYCKEPVRIESKVLESLGFSAMQ
AAGQIYGPVGCEMCNKGYKGRVGIYEVMPISDTMERMILDKASAVDIAEQARLEGVDSIRQSAIKKVVEGVTSIDELLRV
TTD

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=774705 PE074_RS00665 WP_008315358.1 138275..139966(-) (pilB) [Wohlfahrtiimonas chitiniclastica strain BM-Y]
ATGCGATTGATGCACAATGGCCTTGTGAAGGCATTGATGGAAAAAGGATTGGTGACCGAAGCGCACTTAGACCGAGCCGA
TACCATCATGCAGGCCGATAAATGCTCCTTAATGCGAGCATTGATCACCTTGAACATTGCGCCGCAAGCGCTGTTTGAAG
TGACGCAGAGTTTGTCAAACTTGCCGCGTTTTGACTTTAGAGCGTTTGATCTACGCACAAAACCGCCTGAGCTGTTGTCC
ACAGAAGAGATGGAAAAAAACCTTGTATTTCCGCTTCGCATGAAAAATGGCACGCTTTATGTGGCGGTGGCGGATCCGAC
GGATAGCCGCATGCTTGAAACATTGCGCTTTAAGTCACGCGCCATCATTGAGCCCATTTTGGTGGCGCCCATGCAATTGG
TGGCACATTTTGAATCGTATGGCGGCGTGAACCGTGAGAAGTTGCAAAGCATCTTCACCCAAATGGAGACGCAAAAGAAA
GAAGAAACCACAGATCCGGATGCATCGGTCACTTATAACTTAATGAGTGACAAAGACAATTCACCGATCGTGCGCTTCAT
TAATGGCGTATTGGCCGATGCCATTACGCGCGGTGTGTCGGATTTGCACTTTGAACCGTATGAACACAGTTATCGCGTGC
GCTTTCGCATCGATGGTGTTTTGCAAGAGGTGTATACACCGCCAGATAGCTTTAGAGATCAAATTGCGGCGCGTATTAAA
GTCATGTCCCAATTAGACATCACCGAAAAGCGCGTGCCACAAGATGGTCGCATTAAAGTGGATTTGAAAGGTCGCATCAT
TGACTTTCGTGTGAGCATCTGTCCGACCCTTTGGGGCGAGAAGATTGTTATGCGTATTTTAGACAGCTCGGCTGCGAACC
TTAACATCGAGATTTTGGGCTTCAGTGACATTCAAAAGAAAAACATTCTAGAAGCCATTCAAAAGCCACAAGGGATGATT
TTGGTCACAGGTCCCACAGGTTCGGGTAAGACGGTGACGCTTTACACCTGTTTAGGCATTCTCAATCAGCCGACCACCAA
TATTTCCACCGCAGAAGATCCTGTTGAGATCAACTTAGAGGGCATCAATCAGGTGCCAGTGAATCCAAAAGTGGGGCTGA
ACTTTGCGGCGGCATTGAAATCCTTCCTTCGTCAAGATCCGGACATCATCATGGTGGGTGAGATTCGTGACTTAGAAACT
GCGGACATTGCCATTAAAGCGGCGCAAACGGGTCACTTGGTGTTATCAACCTTGCATACAAACTCAGCGCCTGAAACCCT
CACCCGTTTGATCAACATGGGTGTGGAAACCTTTAACATTGCCTCAACTGTGCATTTGATCATCGCCCAGCGCTTAGCCC
GCCGTTTATGTTCTTACTGTAAGGAGCCGGTGCGCATTGAGTCAAAAGTGTTGGAAAGCTTAGGCTTTTCTGCGATGCAA
GCTGCGGGACAAATTTATGGTCCTGTGGGCTGTGAGATGTGTAACAAAGGCTATAAAGGGCGCGTCGGTATTTATGAAGT
GATGCCCATTTCTGATACCATGGAGCGCATGATTTTAGACAAAGCGAGCGCCGTTGATATTGCAGAACAGGCTCGTTTAG
AAGGGGTGGATAGCATTCGTCAATCTGCCATCAAAAAAGTAGTAGAAGGGGTGACCAGCATCGATGAGTTATTGCGTGTC
ACCACGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(14-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

50.174

100

0.512
  pilB Acinetobacter baumannii D1279779

50.439

100

0.51
  pilB Legionella pneumophila strain ERS1305867

50.088

100

0.503
  pilB Vibrio cholerae strain A1552

48.161

100

0.488
  pilF Neisseria gonorrhoeae MS11

47.986

100

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

46.797

99.822

0.467
  pilB Vibrio campbellii strain DS40M4

51.05

84.547

0.432
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.478

100

0.403
  pilF Thermus thermophilus HB27

37.294

97.158

0.362