Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   QUC24_RS26775 Genome accession   NZ_CP128506
Coordinates   5801272..5801895 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain Fe11-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5796272..5806895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUC24_RS26755 (QUC24_26755) aroB 5796867..5797973 (-) 1107 WP_003116138.1 3-dehydroquinate synthase -
  QUC24_RS26760 (QUC24_26760) aroK 5798023..5798541 (-) 519 WP_003095833.1 shikimate kinase AroK -
  QUC24_RS26765 (QUC24_26765) pilQ 5798553..5800697 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  QUC24_RS26770 (QUC24_26770) comL/pilP 5800751..5801275 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  QUC24_RS26775 (QUC24_26775) pilO 5801272..5801895 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  QUC24_RS26780 (QUC24_26780) pilN 5801892..5802488 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  QUC24_RS26785 (QUC24_26785) pilM 5802488..5803552 (-) 1065 WP_034074518.1 type IV pilus assembly protein PilM Machinery gene
  QUC24_RS26790 (QUC24_26790) - 5803737..5806208 (+) 2472 WP_016562439.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=774293 QUC24_RS26775 WP_003103268.1 5801272..5801895(-) (pilO) [Pseudomonas aeruginosa strain Fe11-1]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=774293 QUC24_RS26775 WP_003103268.1 5801272..5801895(-) (pilO) [Pseudomonas aeruginosa strain Fe11-1]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGAGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401