Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7R30_RS03820 Genome accession   NZ_AP022245
Coordinates   792201..793181 (+) Length   326 a.a.
NCBI ID   WP_001295381.1    Uniprot ID   A0A061K2W4
Organism   Escherichia coli strain WP8-S18-CRE-02     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 787201..798181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R30_RS03790 (WP8S18C02_07520) yggM 787716..788723 (+) 1008 WP_001753044.1 DUF1202 family protein -
  H7R30_RS03795 (WP8S18C02_07530) hemW 788878..790014 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  H7R30_RS03800 (WP8S18C02_07540) rdgB 790007..790600 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  H7R30_RS03805 (WP8S18C02_07550) yggU 790608..790898 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  H7R30_RS03810 (WP8S18C02_07560) yggT 790895..791461 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  H7R30_RS03815 (WP8S18C02_07570) yggS 791479..792183 (-) 705 WP_001424369.1 pyridoxal phosphate homeostasis protein -
  H7R30_RS03820 (WP8S18C02_07580) pilT 792201..793181 (+) 981 WP_001295381.1 type IV pilus twitching motility protein PilT Machinery gene
  H7R30_RS25170 - 793230..793358 (+) 129 WP_001278270.1 hypothetical protein -
  H7R30_RS03825 (WP8S18C02_07590) ruvX 793365..793781 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  H7R30_RS03830 (WP8S18C02_07600) yqgE 793781..794344 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  H7R30_RS03835 (WP8S18C02_07610) gshB 794453..795403 (-) 951 WP_000593273.1 glutathione synthase -
  H7R30_RS03840 (WP8S18C02_07620) rsmE 795416..796147 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H7R30_RS03845 (WP8S18C02_07630) endA 796227..796934 (-) 708 WP_000286500.1 deoxyribonuclease I -
  H7R30_RS03850 (WP8S18C02_07640) yggI 797029..797526 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35926.14 Da        Isoelectric Point: 5.7654

>NTDB_id=77359 H7R30_RS03820 WP_001295381.1 792201..793181(+) (pilT) [Escherichia coli strain WP8-S18-CRE-02]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=77359 H7R30_RS03820 WP_001295381.1 792201..793181(+) (pilT) [Escherichia coli strain WP8-S18-CRE-02]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A061K2W4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.912

97.546

0.448
  pilT Legionella pneumophila strain Lp02

45.912

97.546

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

37.048

100

0.377
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment