Detailed information NTG0772893

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PF515_RS14340
• Genome accession: NZ_CP115626
• Coordinates: 2987237..2988355 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 2022CK-00185
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2982237..2993355

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF515_RS14330 (PF515_14330)	-	2985353..2986045 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
PF515_RS14335 (PF515_14335)	pilT	2986172..2987209 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
PF515_RS14340 (PF515_14340)	pilU	2987237..2988355 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PF515_RS14345 (PF515_14345)	fur	2988425..2988862 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
PF515_RS14350 (PF515_14350)	bamE	2988974..2989372 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
PF515_RS14355 (PF515_14355)	-	2989427..2989750 (-)	324	WP_000442587.1	RnfH family protein	-
PF515_RS14360 (PF515_14360)	-	2989750..2990817 (-)	1068	WP_002019572.1	hypothetical protein	-
PF515_RS14365 (PF515_14365)	-	2990970..2991425 (-)	456	WP_000782976.1	bacteriohemerythrin	-
PF515_RS14370 (PF515_14370)	-	2991678..2992526 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=772893 PF515_RS14340 WP_000347039.1 2987237..2988355(+) (pilU) [Acinetobacter baumannii strain 2022CK-00185]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=772893 PF515_RS14340 WP_000347039.1 2987237..2988355(+) (pilU) [Acinetobacter baumannii strain 2022CK-00185]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395