Detailed information NTG0772493

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: PDI73_RS08990
• Genome accession: NZ_CP115479
• Coordinates: 1850152..1850577 (-)
• Length: 141 a.a.
• NCBI ID: WP_161935022.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain EP2
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1845152..1855577

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PDI73_RS08955 (PDI73_08955)	-	1845269..1845646 (-)	378	WP_033900715.1	pyridoxamine 5'-phosphate oxidase family protein	-
PDI73_RS08960 (PDI73_08960)	-	1845851..1846717 (+)	867	WP_033900713.1	RluA family pseudouridine synthase	-
PDI73_RS08965 (PDI73_08965)	-	1846756..1847565 (-)	810	WP_033900712.1	metal ABC transporter permease	-
PDI73_RS08970 (PDI73_08970)	-	1847558..1848295 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
PDI73_RS08975 (PDI73_08975)	-	1848472..1849314 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
PDI73_RS08980 (PDI73_08980)	-	1849311..1849748 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
PDI73_RS08985 (PDI73_08985)	comGG	1849829..1850113 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
PDI73_RS08990 (PDI73_08990)	comGF	1850152..1850577 (-)	426	WP_161935022.1	competence type IV pilus minor pilin ComGF	Machinery gene
PDI73_RS08995 (PDI73_08995)	comGE	1850561..1850857 (-)	297	WP_033900709.1	competence type IV pilus minor pilin ComGE	Machinery gene
PDI73_RS09000 (PDI73_09000)	comGD	1850829..1851245 (-)	417	WP_032941843.1	competence type IV pilus minor pilin ComGD	Machinery gene
PDI73_RS09005 (PDI73_09005)	comGC	1851220..1851489 (-)	270	WP_021721869.1	competence type IV pilus major pilin ComGC	Machinery gene
PDI73_RS09010 (PDI73_09010)	comGB	1851617..1852636 (-)	1020	WP_047206974.1	competence type IV pilus assembly protein ComGB	Machinery gene
PDI73_RS09015 (PDI73_09015)	comGA	1852584..1853522 (-)	939	WP_033900707.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16323.98 Da
• Isoelectric Point: 8.9981

>NTDB_id=772493 PDI73_RS08990 WP_161935022.1 1850152..1850577(-) (comGF) [Lactococcus lactis strain EP2]
MNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFLYVTKDKK
LRFGFMRDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 426 bp

>NTDB_id=772493 PDI73_RS08990 WP_161935022.1 1850152..1850577(-) (comGF) [Lactococcus lactis strain EP2]
TTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAATTTCTGGTTCAGTCCTTGTCAT
CTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAAAAGATTGGCAAATTTTCTGTC
AGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTGTATGTAACTAAAGACAAAAAA
CTAAGGTTTGGTTTTATGAGAGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTATCAGCCAATGCTTTATGATAT
AAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATCAAGGAGGTGAGAGAACATTTA
TTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	98.582	0.752
comYF	Streptococcus mutans UA140	47.826	97.872	0.468
comYF	Streptococcus mutans UA159	47.101	97.872	0.461
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	97.163	0.404
comGF/cglF	Streptococcus mitis SK321	41.606	97.163	0.404
comGF/cglF	Streptococcus pneumoniae Rx1	39.007	100	0.39
comGF/cglF	Streptococcus pneumoniae D39	39.007	100	0.39
comGF/cglF	Streptococcus pneumoniae R6	39.007	100	0.39
comGF/cglF	Streptococcus pneumoniae TIGR4	39.007	100	0.39