Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE   Type   Machinery gene
Locus tag   PDI73_RS08995 Genome accession   NZ_CP115479
Coordinates   1850561..1850857 (-) Length   98 a.a.
NCBI ID   WP_033900709.1    Uniprot ID   A0A0H1RKN4
Organism   Lactococcus lactis strain EP2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1845561..1855857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PDI73_RS08960 (PDI73_08960) - 1845851..1846717 (+) 867 WP_033900713.1 RluA family pseudouridine synthase -
  PDI73_RS08965 (PDI73_08965) - 1846756..1847565 (-) 810 WP_033900712.1 metal ABC transporter permease -
  PDI73_RS08970 (PDI73_08970) - 1847558..1848295 (-) 738 WP_012898617.1 metal ABC transporter ATP-binding protein -
  PDI73_RS08975 (PDI73_08975) - 1848472..1849314 (-) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  PDI73_RS08980 (PDI73_08980) - 1849311..1849748 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  PDI73_RS08985 (PDI73_08985) comGG 1849829..1850113 (-) 285 WP_017865155.1 competence type IV pilus minor pilin ComGG Machinery gene
  PDI73_RS08990 (PDI73_08990) comGF 1850152..1850577 (-) 426 WP_161935022.1 competence type IV pilus minor pilin ComGF Machinery gene
  PDI73_RS08995 (PDI73_08995) comGE 1850561..1850857 (-) 297 WP_033900709.1 competence type IV pilus minor pilin ComGE Machinery gene
  PDI73_RS09000 (PDI73_09000) comGD 1850829..1851245 (-) 417 WP_032941843.1 competence type IV pilus minor pilin ComGD Machinery gene
  PDI73_RS09005 (PDI73_09005) comGC 1851220..1851489 (-) 270 WP_021721869.1 competence type IV pilus major pilin ComGC Machinery gene
  PDI73_RS09010 (PDI73_09010) comGB 1851617..1852636 (-) 1020 WP_047206974.1 competence type IV pilus assembly protein ComGB Machinery gene
  PDI73_RS09015 (PDI73_09015) comGA 1852584..1853522 (-) 939 WP_033900707.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 98 a.a.        Molecular weight: 11048.89 Da        Isoelectric Point: 5.6957

>NTDB_id=772494 PDI73_RS08995 WP_033900709.1 1850561..1850857(-) (comGE) [Lactococcus lactis strain EP2]
MENLKRKSVKAYLLLESLISIALLAFLVSFIVSSLVQVRQKDTEENQKIEALNVAQMAIESHLTELSINGSDIKIKENQN
LLIISNHGKEIMQLELQS

Nucleotide


Download         Length: 297 bp        

>NTDB_id=772494 PDI73_RS08995 WP_033900709.1 1850561..1850857(-) (comGE) [Lactococcus lactis strain EP2]
GTGGAAAATTTAAAAAGAAAATCAGTTAAGGCATATTTGCTTTTGGAAAGTTTAATTAGTATAGCTCTACTCGCTTTTCT
AGTCAGCTTTATCGTAAGTTCTCTTGTGCAAGTAAGACAAAAAGATACGGAAGAAAATCAAAAGATTGAAGCTTTAAACG
TGGCACAAATGGCGATTGAAAGTCATTTAACAGAATTATCAATCAACGGTTCTGATATAAAGATAAAAGAAAACCAAAAT
TTACTGATTATTAGTAATCATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(13-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H1RKN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE Lactococcus lactis subsp. cremoris KW2

67.01

98.98

0.663