Detailed information NTG0769073

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP58_RS31190
• Genome accession: NZ_CP115200
• Coordinates: 6635256..6636404 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F073
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6630256..6641404

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP58_RS31170 (PCP58_31160)	-	6630994..6632178 (-)	1185	WP_015649763.1	cystathionine gamma-synthase	-
PCP58_RS31175 (PCP58_31165)	-	6632175..6633548 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PCP58_RS31180 (PCP58_31170)	-	6633791..6634195 (-)	405	WP_003084560.1	NINE protein	-
PCP58_RS31185 (PCP58_31175)	czcD	6634349..6635248 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP58_RS31190 (PCP58_31180)	pilU	6635256..6636404 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PCP58_RS31195 (PCP58_31185)	pilT	6636582..6637616 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP58_RS31200 (PCP58_31190)	-	6637831..6638523 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP58_RS31205 (PCP58_31195)	proC	6638535..6639356 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP58_RS31210 (PCP58_31200)	-	6639367..6639960 (+)	594	WP_003084544.1	YggT family protein	-
PCP58_RS31215 (PCP58_31205)	-	6639957..6640253 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=769073 PCP58_RS31190 WP_003100959.1 6635256..6636404(-) (pilU) [Pseudomonas aeruginosa strain F073]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=769073 PCP58_RS31190 WP_003100959.1 6635256..6636404(-) (pilU) [Pseudomonas aeruginosa strain F073]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGATCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382