Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   LLE01_RS07330 Genome accession   NZ_CP126675
Coordinates   1221631..1223514 (+) Length   627 a.a.
NCBI ID   WP_003130801.1    Uniprot ID   Q9CEF5
Organism   Lactococcus lactis subsp. lactis strain E.0.1     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 1216631..1228514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLE01_RS07300 (LLE01_07280) - 1217748..1217987 (+) 240 WP_260307335.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  LLE01_RS07305 (LLE01_07285) amaP 1218015..1218575 (+) 561 WP_010906186.1 alkaline shock response membrane anchor protein AmaP -
  LLE01_RS07310 (LLE01_07290) - 1218586..1218774 (+) 189 WP_012898445.1 DUF2273 domain-containing protein -
  LLE01_RS07315 (LLE01_07295) - 1218795..1219301 (+) 507 WP_003131479.1 Asp23/Gls24 family envelope stress response protein -
  LLE01_RS07320 (LLE01_07300) rsmB 1219500..1220774 (+) 1275 WP_010906185.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  LLE01_RS07325 (LLE01_07305) - 1220855..1221631 (+) 777 WP_003130802.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  LLE01_RS07330 (LLE01_07310) pknB 1221631..1223514 (+) 1884 WP_003130801.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  LLE01_RS07335 (LLE01_07315) rpsO 1223696..1223965 (+) 270 WP_010906184.1 30S ribosomal protein S15 -
  LLE01_RS07340 (LLE01_07320) - 1224102..1225049 (+) 948 WP_003130410.1 IS30 family transposase -
  LLE01_RS07345 (LLE01_07325) - 1225064..1225894 (+) 831 WP_010905507.1 polysaccharide deacetylase family protein -
  LLE01_RS07350 (LLE01_07330) - 1225921..1226166 (+) 246 WP_010905508.1 hypothetical protein -
  LLE01_RS07355 (LLE01_07335) - 1226163..1227173 (+) 1011 WP_010905509.1 collagen binding domain-containing protein -
  LLE01_RS07360 (LLE01_07340) - 1227240..1227374 (+) 135 WP_003129614.1 LPXTG cell wall anchor domain-containing protein -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 67877.92 Da        Isoelectric Point: 4.7783

>NTDB_id=768088 LLE01_RS07330 WP_003130801.1 1221631..1223514(+) (pknB) [Lactococcus lactis subsp. lactis strain E.0.1]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVGNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLSQAKSKIKDAKLKVGTVHKQQSSTI
AEGKVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPNVGT
SVDPTQEIVVTYSVYTAPSSSSTTSESTTTSETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=768088 LLE01_RS07330 WP_003130801.1 1221631..1223514(+) (pknB) [Lactococcus lactis subsp. lactis strain E.0.1]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAGCTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATTAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGATCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGGAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CACTTAGCCAAGCAAAAAGCAAAATTAAAGATGCAAAACTTAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAAAGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATCTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCTGATGGTTATGTTTACTCAGTCTATCCAAATGTAGGGACA
TCTGTTGATCCAACTCAAGAAATTGTAGTCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACTACGACGTCTGAAACTTCATCAAGTACAACTTCAAGTACTTCAAGCTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(12-265)

PASTA domain (PASTA)

(365-428)

PASTA domain (PASTA)

(506-573)

PASTA domain (PASTA)

(434-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CEF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

49.265

86.762

0.427
  stkP/pknB Streptococcus salivarius strain HSISS4

51.445

82.775

0.426
  stkP Streptococcus pneumoniae TIGR4

48.024

80.702

0.388
  stkP Streptococcus pneumoniae D39

47.826

80.702

0.386
  stkP Streptococcus pneumoniae R6

47.826

80.702

0.386
  stkP Streptococcus pneumoniae Rx1

47.628

80.702

0.384