Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   ICA20_RS00535 Genome accession   NZ_CP115032
Coordinates   102864..103349 (-) Length   161 a.a.
NCBI ID   WP_000157061.1    Uniprot ID   A0AAU7IP99
Organism   Citrobacter sp. C5_2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 65162..112062 102864..103349 within 0


Gene organization within MGE regions


Location: 65162..112062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICA20_RS00325 (ICA20_000325) aadA1 65526..66364 (-) 839 Protein_64 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -
  ICA20_RS00330 (ICA20_000330) - 66477..67307 (-) 831 WP_001334766.1 oxacillin-hydrolyzing class D beta-lactamase OXA-1 -
  ICA20_RS00335 (ICA20_000335) intI1 67517..68530 (+) 1014 WP_000845048.1 class 1 integron integrase IntI1 -
  ICA20_RS00340 (ICA20_000340) - 68733..69083 (+) 351 Protein_67 DUF3330 domain-containing protein -
  ICA20_RS00345 (ICA20_000345) - 69209..69769 (+) 561 WP_000147567.1 recombinase family protein -
  ICA20_RS00350 (ICA20_000350) - 69772..72738 (+) 2967 WP_001138064.1 Tn3-like element TnAs3 family transposase -
  ICA20_RS00355 (ICA20_000355) - 72805..73182 (+) 378 WP_000656305.1 GNAT family N-acetyltransferase -
  ICA20_RS00360 (ICA20_000360) - 73383..74042 (-) 660 WP_000412211.1 type A-1 chloramphenicol O-acetyltransferase -
  ICA20_RS00365 (ICA20_000365) - 74321..75018 (+) 698 WP_095033700.1 IS1-like element IS1B family transposase -
  ICA20_RS00370 (ICA20_000370) - 75015..75278 (+) 264 WP_049802299.1 antibiotic biosynthesis monooxygenase -
  ICA20_RS00375 (ICA20_000375) - 75271..75657 (+) 387 WP_000460651.1 hypothetical protein -
  ICA20_RS00380 (ICA20_000380) - 75665..76351 (+) 687 WP_001284954.1 winged helix-turn-helix domain-containing protein -
  ICA20_RS00385 (ICA20_000385) tetR(B) 76329..76952 (-) 624 WP_000088605.1 tetracycline resistance transcriptional repressor TetR(B) -
  ICA20_RS00390 (ICA20_000390) tet(B) 77034..78239 (+) 1206 WP_129694983.1 tetracycline efflux MFS transporter Tet(B) -
  ICA20_RS00395 (ICA20_000395) tetC 78352..78945 (-) 594 WP_000428546.1 tetracyline resistance-associated transcriptional repressor TetC -
  ICA20_RS00400 (ICA20_000400) - 79033..79449 (+) 417 WP_000275180.1 helix-turn-helix domain-containing protein -
  ICA20_RS00405 (ICA20_000405) - 79459..80622 (-) 1164 Protein_80 IS4-like element ISVsa5 family transposase -
  ICA20_RS00415 - 81689..81775 (+) 87 WP_089627921.1 transposase -
  ICA20_RS00420 (ICA20_000420) - 81971..82291 (-) 321 Protein_83 GTPase -
  ICA20_RS00425 (ICA20_000425) - 82375..83292 (-) 918 WP_135113592.1 hypothetical protein -
  ICA20_RS00430 - 84389..84585 (+) 197 Protein_85 hypothetical protein -
  ICA20_RS00435 (ICA20_000430) - 84491..85093 (-) 603 WP_149282298.1 hypothetical protein -
  ICA20_RS00440 (ICA20_000435) - 85187..85393 (-) 207 WP_179957931.1 AlpA family phage regulatory protein -
  ICA20_RS00445 (ICA20_000440) hha 86118..86330 (+) 213 WP_087901481.1 hemolysin expression modulator Hha -
  ICA20_RS00450 (ICA20_000445) - 86963..87532 (+) 570 WP_123001625.1 inovirus Gp2 family protein -
  ICA20_RS00455 (ICA20_000450) - 87792..88175 (+) 384 WP_143361722.1 putative zinc ribbon protein -
  ICA20_RS00460 (ICA20_000455) - 88172..88597 (+) 426 WP_087901757.1 putative zinc ribbon protein -
  ICA20_RS00465 (ICA20_000460) - 89250..89345 (+) 96 Protein_92 AlpA family transcriptional regulator -
  ICA20_RS00470 (ICA20_000465) - 89346..90320 (-) 975 Protein_93 IS66 family transposase -
  ICA20_RS00475 (ICA20_000470) tnpB 90345..90680 (-) 336 Protein_94 IS66 family insertion sequence element accessory protein TnpB -
  ICA20_RS00480 (ICA20_000475) - 90667..90996 (-) 330 WP_040235205.1 hypothetical protein -
  ICA20_RS00485 (ICA20_000480) - 91103..92020 (-) 918 WP_087901758.1 LysR substrate-binding domain-containing protein -
  ICA20_RS00490 (ICA20_000485) dcuC 92156..93427 (+) 1272 WP_040235202.1 C4-dicarboxylate transporter DcuC -
  ICA20_RS00495 (ICA20_000490) - 93440..94750 (+) 1311 WP_087901759.1 amidohydrolase -
  ICA20_RS00500 (ICA20_000495) - 95250..95675 (+) 426 WP_038635492.1 transposase -
  ICA20_RS00505 (ICA20_000500) tnpB 95672..96022 (+) 351 WP_038635488.1 IS66 family insertion sequence element accessory protein TnpB -
  ICA20_RS00510 (ICA20_000505) - 96053..97477 (+) 1425 Protein_101 IS66 family transposase -
  ICA20_RS00515 (ICA20_000510) - 97708..98446 (+) 739 Protein_102 IS3 family transposase -
  ICA20_RS00520 (ICA20_000515) ltrA 99119..100627 (+) 1509 WP_038635474.1 group II intron reverse transcriptase/maturase -
  ICA20_RS00525 (ICA20_000520) - 100717..101190 (+) 474 Protein_104 IS3 family transposase -
  ICA20_RS00530 (ICA20_000525) - 101231..102813 (-) 1583 Protein_105 conjugal transfer protein TrbC -
  ICA20_RS00535 (ICA20_000530) ssb 102864..103349 (-) 486 WP_000157061.1 single-stranded DNA-binding protein Machinery gene
  ICA20_RS00540 (ICA20_000535) - 103997..106378 (-) 2382 WP_038635468.1 glycoside hydrolase family 31 protein -
  ICA20_RS00545 (ICA20_000540) - 106393..107694 (-) 1302 WP_001329026.1 MFS transporter -
  ICA20_RS00550 (ICA20_000545) - 107956..109026 (+) 1071 WP_000412404.1 LacI family DNA-binding transcriptional regulator -
  ICA20_RS00555 (ICA20_000550) - 109424..110467 (-) 1044 WP_000999850.1 hypothetical protein -

Sequence


Protein


Download         Length: 161 a.a.        Molecular weight: 17685.62 Da        Isoelectric Point: 7.2017

>NTDB_id=767947 ICA20_RS00535 WP_000157061.1 102864..103349(-) (ssb) [Citrobacter sp. C5_2]
MASKGINKVILIGYLGQDPDIRYMPNGGAVASIALATSETWRDKQTGEMREQTEWHRVVLFGKLAEVASEYLRKGAQVYI
DGQLRTRKWTDQTGQERYTTEVVVHTGGTMQMLGSRQNSAQGGRGQPQQPQQGRQFSGNSPSPGNTAMSGSFSDDDDSIP
F

Nucleotide


Download         Length: 486 bp        

>NTDB_id=767947 ICA20_RS00535 WP_000157061.1 102864..103349(-) (ssb) [Citrobacter sp. C5_2]
ATGGCCTCTAAAGGTATTAACAAAGTTATTCTGATAGGGTATCTGGGTCAGGACCCGGATATTCGCTATATGCCAAACGG
CGGCGCTGTGGCCAGCATTGCGCTGGCCACCTCCGAAACCTGGCGGGACAAACAGACAGGGGAGATGCGGGAACAAACCG
AATGGCACCGCGTGGTGCTGTTCGGCAAACTGGCGGAAGTAGCCAGTGAATACCTGCGCAAAGGAGCTCAGGTGTACATC
GATGGTCAGCTGCGTACCCGAAAATGGACCGACCAGACCGGCCAGGAACGCTACACGACTGAAGTGGTGGTGCATACCGG
CGGTACCATGCAGATGCTGGGCTCCCGGCAGAATAGTGCACAGGGTGGCCGGGGACAGCCTCAACAACCACAACAGGGCA
GACAGTTTTCAGGTAATTCGCCATCTCCGGGCAATACCGCCATGTCCGGCAGTTTCAGCGACGATGACGACAGTATCCCG
TTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

58.757

100

0.646

  ssb Glaesserella parasuis strain SC1401

52.632

94.41

0.497

  ssb Neisseria meningitidis MC58

41.477

100

0.453

  ssb Neisseria gonorrhoeae MS11

41.477

100

0.453

  ssb Latilactobacillus sakei subsp. sakei 23K

32.402

100

0.36