Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   QN227_RS03725 Genome accession   NZ_CP126348
Coordinates   771879..773372 (+) Length   497 a.a.
NCBI ID   WP_033557387.1    Uniprot ID   -
Organism   Escherichia coli strain NN5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 766879..778372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN227_RS03705 (QN227_03705) pilD 767527..768336 (+) 810 WP_001297673.1 prepilin peptidase PppA Machinery gene
  QN227_RS03710 (QN227_03710) gspS2 768402..768812 (+) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  QN227_RS03715 (QN227_03715) gspC 768830..769789 (+) 960 WP_000135063.1 type II secretion system protein GspC -
  QN227_RS03720 (QN227_03720) gspD 769819..771879 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  QN227_RS03725 (QN227_03725) pilB/pilB1 771879..773372 (+) 1494 WP_033557387.1 type II secretion system ATPase GspE Machinery gene
  QN227_RS03730 (QN227_03730) gspF 773372..774595 (+) 1224 WP_033557386.1 type II secretion system inner membrane protein GspF -
  QN227_RS03735 (QN227_03735) gspG 774612..775067 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  QN227_RS03740 (QN227_03740) gspH 775071..775634 (+) 564 WP_001115140.1 type II secretion system minor pseudopilin GspH -
  QN227_RS03745 (QN227_03745) gspI 775631..776002 (+) 372 WP_000820125.1 type II secretion system minor pseudopilin GspI -
  QN227_RS03750 (QN227_03750) gspJ 775999..776598 (+) 600 WP_001255040.1 type II secretion system minor pseudopilin GspJ -
  QN227_RS03755 (QN227_03755) gspK 776601..777578 (+) 978 WP_000633209.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55236.40 Da        Isoelectric Point: 6.0390

>NTDB_id=767252 QN227_RS03725 WP_033557387.1 771879..773372(+) (pilB/pilB1) [Escherichia coli strain NN5]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
ETRQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=767252 QN227_RS03725 WP_033557387.1 771879..773372(+) (pilB/pilB1) [Escherichia coli strain NN5]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGTTGTCACTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGACCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGATGCGCCGATCATCAAACTGATTAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGC
CCTGGGCGCAAACTGGCCTCGCTGCTGGTCTCACGAATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACA
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGACCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATTGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGGGTAGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGTACGCTGTGCCCCGACTGCCGCCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilB Acinetobacter baumannii D1279779

46.115

80.282

0.37
  pilF Thermus thermophilus HB27

45

80.483

0.362
  pilB Acinetobacter baylyi ADP1

45.897

78.471

0.36