Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O5O48_RS01205 Genome accession   NZ_CP114836
Coordinates   266243..267943 (+) Length   566 a.a.
NCBI ID   WP_269687931.1    Uniprot ID   -
Organism   Halopseudomonas sp. MFKK-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 254141..265447 266243..267943 flank 796


Gene organization within MGE regions


Location: 254141..267943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O5O48_RS01150 - 254204..255298 (+) 1095 WP_269687920.1 glycosyltransferase family 4 protein -
  O5O48_RS01155 - 255300..256340 (+) 1041 WP_269687921.1 glycosyltransferase family 4 protein -
  O5O48_RS01160 - 256314..257195 (-) 882 WP_269687922.1 glycosyltransferase -
  O5O48_RS01165 - 257203..258660 (-) 1458 WP_269687923.1 MOP flippase family protein -
  O5O48_RS01170 - 258657..259337 (-) 681 WP_269687924.1 methyltransferase domain-containing protein -
  O5O48_RS01175 - 259343..259987 (-) 645 WP_269687925.1 class I SAM-dependent methyltransferase -
  O5O48_RS01180 - 259984..261150 (-) 1167 WP_269687926.1 glycosyltransferase -
  O5O48_RS01185 - 261347..262513 (-) 1167 WP_269687927.1 nucleotide sugar dehydrogenase -
  O5O48_RS01190 - 262605..263480 (-) 876 WP_269687928.1 glycosyltransferase family 2 protein -
  O5O48_RS01195 - 263477..265447 (-) 1971 WP_269687929.1 hypothetical protein -
  O5O48_RS01200 - 265527..265949 (-) 423 WP_269687930.1 pilin -
  O5O48_RS01205 pilB 266243..267943 (+) 1701 WP_269687931.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62347.63 Da        Isoelectric Point: 5.2426

>NTDB_id=767214 O5O48_RS01205 WP_269687931.1 266243..267943(+) (pilB) [Halopseudomonas sp. MFKK-1]
METPALSGLARRIVQEGLIQADVAAKACQQASQDKIPLITHLVQKKLADARSLAMTCAEEFGYPYFDLSALDKEAQPKEL
VSEKLVRQHNVMPLFKRGSRLYLAISDPTNQQALSDVQFHTGLMTDAVIVEDDKLLSAIEKYLDSPTGGLGDIGDADLDE
LDVQSVKDEDRADGGTDADDAPIVRFVNKMLLDAVRMGSSDLHFEPYEKMYRVRFRTDGVLHEVAKPPVQLGVRIAARLK
VMSSMDLAERRKPQDGRIKLKISKSKAIDFRVNTLPTLWGEKVVLRILDSESAKMGIDALGYEDDQKQMYMAALARPQGM
ILVTGPTGSGKTVSLYTGLNILNTMERNISTAEDPVEINLEGINQVNVNPKQGLDFAQALRAFLRQDPDIIMVGEIRDLE
TAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARRLCKSCKVPMDVPREVLLEEGFTPE
KIDAGITIYGPVGCENCKDGYKGRVGIYEVVKITPAMQRIIMEDGNSIQISDVAQSEGFRNLRQSALMKAEEGVTSLAEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=767214 O5O48_RS01205 WP_269687931.1 266243..267943(+) (pilB) [Halopseudomonas sp. MFKK-1]
ATGGAAACCCCCGCTCTTTCAGGGCTCGCACGGCGTATTGTGCAGGAAGGCCTTATTCAGGCCGACGTGGCTGCGAAAGC
CTGCCAGCAGGCTAGTCAGGACAAAATTCCGCTGATCACCCATCTGGTGCAGAAGAAGCTGGCAGATGCCCGCTCGCTGG
CAATGACCTGCGCTGAAGAATTCGGCTACCCCTACTTCGACCTCAGCGCTCTGGACAAGGAAGCTCAACCCAAGGAGCTG
GTCAGTGAGAAGCTGGTGCGCCAGCACAATGTGATGCCGCTGTTCAAGCGCGGCTCACGCCTGTATCTGGCCATTTCTGA
TCCAACAAATCAGCAGGCCCTCAGTGATGTTCAGTTCCACACCGGCCTGATGACCGACGCGGTGATCGTCGAAGACGACA
AGCTGCTGAGCGCTATCGAAAAATACCTCGACAGCCCGACCGGTGGCTTGGGCGACATTGGCGATGCAGATCTGGACGAG
CTTGACGTTCAATCGGTCAAGGATGAAGACCGTGCGGACGGAGGCACTGACGCCGACGACGCGCCCATAGTTCGCTTCGT
CAACAAGATGCTGCTTGACGCCGTGCGCATGGGTTCATCTGACCTGCACTTTGAACCCTATGAAAAGATGTACCGTGTAC
GTTTCCGTACCGACGGCGTGCTGCACGAGGTAGCCAAGCCTCCCGTGCAGCTGGGCGTGCGTATCGCTGCGCGCCTCAAA
GTGATGTCATCCATGGACCTGGCTGAGCGCCGCAAGCCGCAAGATGGCCGCATCAAGCTGAAGATTTCCAAGAGCAAGGC
CATCGACTTCCGGGTCAATACCCTGCCGACGCTGTGGGGCGAGAAGGTGGTGCTGCGTATTCTCGACTCCGAAAGCGCCA
AGATGGGTATCGACGCGCTGGGTTATGAAGACGATCAGAAGCAGATGTATATGGCGGCCCTTGCCAGACCGCAGGGCATG
ATTCTGGTTACCGGCCCGACAGGCTCGGGCAAAACCGTATCGCTGTATACCGGCCTGAACATCCTGAACACCATGGAGCG
CAATATCTCCACCGCCGAGGACCCGGTCGAAATCAACCTTGAGGGCATCAACCAGGTCAACGTAAACCCCAAGCAGGGTC
TGGATTTTGCCCAGGCGCTGCGCGCCTTCCTGCGTCAGGACCCGGACATCATCATGGTGGGTGAGATCCGCGACCTGGAA
ACCGCCGAAATCGCGATCAAGGCCGCACAGACCGGCCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGAGAC
GCTGACGCGTCTGCGCAACATGGGTGTGCCCTCGTTCAACATCGCCACCTCGGTCAACCTGATCATCGCCCAGCGTCTGG
CAAGAAGGCTGTGCAAGTCCTGCAAGGTGCCCATGGATGTGCCCCGCGAGGTGCTGCTGGAAGAAGGTTTCACACCGGAA
AAAATTGATGCAGGCATAACAATTTACGGCCCTGTGGGTTGCGAAAACTGTAAAGACGGTTATAAAGGTCGTGTTGGTAT
CTATGAGGTTGTCAAGATCACTCCGGCGATGCAGCGCATCATCATGGAAGACGGCAACTCCATCCAGATTTCCGACGTAG
CCCAGAGTGAGGGTTTCCGAAACTTGCGCCAGTCCGCGCTGATGAAGGCGGAAGAAGGTGTGACCAGCCTGGCGGAAGTC
AACCGAGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.175

100

0.606
  pilB Acinetobacter baylyi ADP1

59.752

99.647

0.595
  pilB Legionella pneumophila strain ERS1305867

56.79

100

0.569
  pilB Vibrio cholerae strain A1552

50.709

99.647

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

49.466

99.293

0.491
  pilF Neisseria gonorrhoeae MS11

48.759

99.647

0.486
  pilB Vibrio campbellii strain DS40M4

47.979

100

0.482
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.462

98.763

0.38
  pilF Thermus thermophilus HB27

36.46

99.823

0.364