Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LpnH3D14_RS07740 Genome accession   NZ_CP114576
Coordinates   1682210..1683934 (+) Length   574 a.a.
NCBI ID   WP_027219449.1    Uniprot ID   A0A378K7Q9
Organism   Legionella pneumophila strain H3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1677210..1688934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LpnH3D14_RS07720 (LpnH3D14_01547) - 1677545..1680073 (+) 2529 WP_027219452.1 EAL domain-containing protein -
  LpnH3D14_RS07725 (LpnH3D14_01548) - 1680075..1680644 (-) 570 WP_011213801.1 hypoxanthine-guanine phosphoribosyltransferase -
  LpnH3D14_RS07730 (LpnH3D14_01549) - 1680687..1681013 (-) 327 WP_027219451.1 DUF1820 family protein -
  LpnH3D14_RS07735 (LpnH3D14_01550) - 1681196..1681939 (-) 744 WP_027219450.1 methyltransferase domain-containing protein -
  LpnH3D14_RS07740 (LpnH3D14_01551) pilB 1682210..1683934 (+) 1725 WP_027219449.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LpnH3D14_RS07745 (LpnH3D14_01552) pilC 1683940..1685160 (+) 1221 WP_027219448.1 type II secretion system F family protein Machinery gene
  LpnH3D14_RS07750 (LpnH3D14_01553) - 1685206..1686069 (+) 864 WP_027219447.1 prepilin peptidase -
  LpnH3D14_RS07755 (LpnH3D14_01554) - 1686083..1687288 (-) 1206 WP_027223897.1 cyclic nucleotide-binding domain-containing protein -
  LpnH3D14_RS07760 (LpnH3D14_01555) - 1687396..1687989 (-) 594 WP_011946528.1 hypothetical protein -
  LpnH3D14_RS07765 (LpnH3D14_01556) - 1688005..1688460 (-) 456 WP_011946529.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62861.48 Da        Isoelectric Point: 5.4444

>NTDB_id=766458 LpnH3D14_RS07740 WP_027219449.1 1682210..1683934(+) (pilB) [Legionella pneumophila strain H3]
MALATEEYRLQGIGQLLVMEKLLDKDKAIELHKLAAAEKMSLVQYLVKNKILSADQIALTAAQNFGVPMMDINCIDMESI
PVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSYFIDNLLTAKESQGLSGYVE
DSGDLEGLEISSEDEEQEVDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLA
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDPGAAKLGIEALGFNPVQKSNFIK
AIERPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETADIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTSQG
LIELGFKEADLVNLKLYKAVGCDQCKSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=766458 LpnH3D14_RS07740 WP_027219449.1 1682210..1683934(+) (pilB) [Legionella pneumophila strain H3]
ATGGCTCTAGCTACAGAAGAATATAGGTTACAAGGAATTGGACAGCTTCTCGTGATGGAAAAGCTGCTAGATAAAGACAA
AGCAATTGAATTACACAAATTGGCAGCAGCAGAAAAAATGTCTTTAGTGCAATACCTTGTAAAAAATAAAATATTATCAG
CTGATCAAATTGCATTGACAGCTGCTCAAAATTTTGGCGTCCCAATGATGGATATTAATTGTATTGACATGGAATCCATT
CCTGTCAATCTGGTTAATGAAAAATTAATTAAACGACATGCCATGGTCCCACTATTTAGTCGAGGCACCAACTTATACCT
TGCAACAGATGATCCTAGCAAGCAAGCTTCACTAAAGGAAATTCAATTTCATACTGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGCTATTTCATTGATAACTTATTGACAGCAAAAGAAAGTCAGGGCTTGTCAGGCTATGTCGAA
GATTCTGGGGATCTGGAAGGTTTAGAAATAAGTTCTGAAGATGAAGAACAAGAGGTTGATACAGCAACATCAGTAACGGA
TGATGCCCCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGGGCTTCTGATATTCACTTTGAGC
CTTATGAACGAGAATACCGAATCAGGTACAGGCAAGATGGTATTTTGCACGAGGTAGCCACCCCGCCGGCAAGCTTGGCA
TCTCGAATTACAGCACGCATCAAGGTAATGTCTAATTTGGATATATCAGAGCGACGGATACCCCAAGATGGCGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCTATTGATTTCAGGGTGAGTACATGCCCGACCTCAGCTGGGGAAAAAGTGGTTATGC
GGGTCCTTGATCCTGGTGCTGCTAAATTAGGGATTGAAGCTTTAGGATTTAATCCTGTCCAGAAGTCAAATTTTATTAAA
GCAATTGAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACAGGTAGTGGGAAAACAGTCACTTTGTATACAGCGTT
AAATATATTGAATACAATTGAAGTTAATATTTCAACAGCTGAAGATCCGGTTGAAATTAAAGTACCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCTTTGCGTTCGTTTCTAAGGCAAGATCCTGATATTATTATG
GTTGGCGAGATACGAGATCTTGAAACAGCTGACATTGCAGTTAAAGCTGCTCAAACAGGGCATTTGGTTCTTTCTACTCT
GCATACCAACAGTGCTGCTGAAACATTAAATCGTTTGGTGAACATGGGGATACCTACTTTTAATATTGCGAGTTCGGTTA
CCTTGATTATTGCGCAAAGGCTGGCAAGAAAATTATGTAATCAATGTAAGGCAGTGCGGGATGACTTTACCAGTCAAGGT
TTGATTGAATTAGGTTTCAAGGAGGCTGATCTGGTTAATCTTAAATTATATAAAGCGGTAGGTTGTGATCAATGTAAAAG
TGGTTATCGTGGGCGAGTTGGTTTATTTGAGGTTTTACCGATGACGAAAGAGCTCGGCCAATTGATTATGTCAGGTGGTA
ACTCGCTGGATATATTAAAGTTAGCTCAGTCAGAAGGTATGCTGACTATCTTTCAATCGGGAATAGAAAAAGTTAAGGAA
GGGATTACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378K7Q9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

94.948

100

0.949
  pilB Acinetobacter baylyi ADP1

50.256

100

0.512
  pilB Acinetobacter baumannii D1279779

53.235

94.251

0.502
  pilB Vibrio cholerae strain A1552

49.908

94.251

0.47
  pilF Neisseria gonorrhoeae MS11

46.82

98.606

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

48.134

93.38

0.449
  pilB Vibrio campbellii strain DS40M4

47.584

93.728

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

92.16

0.368
  pilF Thermus thermophilus HB27

36.632

100

0.368