Detailed information NTG0766450

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LpnH3D14_RS05190
• Genome accession: NZ_CP114576
• Coordinates: 1118527..1119591 (+)
• Length: 354 a.a.
• NCBI ID: WP_014843599.1
• Uniprot ID: A0A4T1VG56
• Organism: Legionella pneumophila strain H3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1113527..1124591

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LpnH3D14_RS05180 (LpnH3D14_01037)	-	1114567..1116951 (-)	2385	WP_014843597.1	penicillin-binding protein 1A	-
LpnH3D14_RS05185 (LpnH3D14_01038)	-	1117103..1118110 (-)	1008	WP_269565295.1	hypothetical protein	-
LpnH3D14_RS05190 (LpnH3D14_01039)	pilM	1118527..1119591 (+)	1065	WP_014843599.1	type IV pilus assembly protein PilM	Machinery gene
LpnH3D14_RS05195 (LpnH3D14_01040)	pilN	1119595..1120143 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
LpnH3D14_RS05200 (LpnH3D14_01041)	pilO	1120157..1120753 (+)	597	WP_027224065.1	type 4a pilus biogenesis protein PilO	Machinery gene
LpnH3D14_RS05205 (LpnH3D14_01042)	pilP	1120750..1121334 (+)	585	WP_269565294.1	pilus assembly protein PilP	Machinery gene
LpnH3D14_RS05210 (LpnH3D14_01043)	pilQ	1121338..1123434 (+)	2097	WP_269565293.1	type IV pilus secretin PilQ	Machinery gene
LpnH3D14_RS05215 (LpnH3D14_01044)	aroK	1123879..1124406 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38776.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=766450 LpnH3D14_RS05190 WP_014843599.1 1118527..1119591(+) (pilM) [Legionella pneumophila strain H3]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLASLIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=766450 LpnH3D14_RS05190 WP_014843599.1 1118527..1119591(+) (pilM) [Legionella pneumophila strain H3]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCGGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGCCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCATCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A4T1VG56

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.718	100	0.997
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407