Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LpnH3D14_RS05200 Genome accession   NZ_CP114576
Coordinates   1120157..1120753 (+) Length   198 a.a.
NCBI ID   WP_027224065.1    Uniprot ID   A0AAP3HB18
Organism   Legionella pneumophila strain H3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1115157..1125753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LpnH3D14_RS05185 (LpnH3D14_01038) - 1117103..1118110 (-) 1008 WP_269565295.1 hypothetical protein -
  LpnH3D14_RS05190 (LpnH3D14_01039) pilM 1118527..1119591 (+) 1065 WP_014843599.1 type IV pilus assembly protein PilM Machinery gene
  LpnH3D14_RS05195 (LpnH3D14_01040) pilN 1119595..1120143 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  LpnH3D14_RS05200 (LpnH3D14_01041) pilO 1120157..1120753 (+) 597 WP_027224065.1 type 4a pilus biogenesis protein PilO Machinery gene
  LpnH3D14_RS05205 (LpnH3D14_01042) pilP 1120750..1121334 (+) 585 WP_269565294.1 pilus assembly protein PilP Machinery gene
  LpnH3D14_RS05210 (LpnH3D14_01043) pilQ 1121338..1123434 (+) 2097 WP_269565293.1 type IV pilus secretin PilQ Machinery gene
  LpnH3D14_RS05215 (LpnH3D14_01044) aroK 1123879..1124406 (+) 528 WP_010946667.1 shikimate kinase AroK -
  LpnH3D14_RS05220 (LpnH3D14_01045) aroB 1124393..1125502 (+) 1110 WP_013101269.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22436.16 Da        Isoelectric Point: 5.3369

>NTDB_id=766452 LpnH3D14_RS05200 WP_027224065.1 1120157..1120753(+) (pilO) [Legionella pneumophila strain H3]
MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 597 bp        

>NTDB_id=766452 LpnH3D14_RS05200 WP_027224065.1 1120157..1120753(+) (pilO) [Legionella pneumophila strain H3]
ATGGACACGGTCAATTTAAATGAATTAACTCTGGAGAATGTTGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTAATTTT
AGGCATCAGTATACTGATAATTGGATTAGGATATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAATTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCCTATCGTAATCAGCTA
CAGTTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTTCCGGCGAAAAATGAAATGCCTGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATCGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAGGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTAGCTGAAATGAACAGA
ATAGTGACTTTGCATGAATTTAGTGTTGAGGGGGTGTCCGGGGATGGTAAAACGGTCTCTCAGGATGAATTGGTGATGGC
TATCACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

99.497

100

1
  comO Acinetobacter baylyi ADP1

40.526

95.96

0.389