Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   QM970_RS13910 Genome accession   NZ_CP125980
Coordinates   2578026..2579030 (-) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis strain ZKY03     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2573026..2584030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QM970_RS13880 (QM970_13890) yrbG 2574000..2574656 (-) 657 WP_014480436.1 DUF421 domain-containing protein -
  QM970_RS13885 (QM970_13895) - 2574854..2575243 (+) 390 WP_014477528.1 TIGR04086 family membrane protein -
  QM970_RS13890 (QM970_13900) yajC 2575300..2575566 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  QM970_RS13895 (QM970_13905) tgt 2575603..2576748 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  QM970_RS13900 (QM970_13910) queA 2576775..2577803 (-) 1029 WP_014477530.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  QM970_RS13905 (QM970_13915) yrzS 2577833..2578033 (-) 201 WP_003222669.1 DUF2905 domain-containing protein -
  QM970_RS13910 (QM970_13920) ruvB 2578026..2579030 (-) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  QM970_RS13915 (QM970_13925) ruvA 2579041..2579646 (-) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  QM970_RS13920 (QM970_13930) bofC 2579785..2580297 (-) 513 WP_014480438.1 sporulation cell-cell signaling protein BofC -
  QM970_RS13925 (QM970_13935) csbX 2580345..2581652 (-) 1308 WP_014480439.1 MFS transporter -
  QM970_RS13930 (QM970_13940) iolG 2581723..2582748 (-) 1026 WP_014480440.1 inositol 2-dehydrogenase -
  QM970_RS13935 (QM970_13945) yrzF 2582986..2583633 (+) 648 WP_014480441.1 phosphotransferase -
  QM970_RS13940 (QM970_13950) - 2583679..2583801 (-) 123 WP_003229707.1 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=765576 QM970_RS13910 WP_003229718.1 2578026..2579030(-) (ruvB) [Bacillus subtilis strain ZKY03]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=765576 QM970_RS13910 WP_003229718.1 2578026..2579030(-) (ruvB) [Bacillus subtilis strain ZKY03]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACTACGCTTGCTTCGATTGTTGCCAATGAAATGGGAGTGGAG
CTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGTTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACA
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTCATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGTGATTTCGCCCAAGTGCTGGGTGACAGCAGGATC
ACTGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGATCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATATCAGCTACGATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518