Detailed information NTG0763354

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OXH74_RS09890
• Genome accession: NZ_CP113889
• Coordinates: 2158158..2159303 (+)
• Length: 381 a.a.
• NCBI ID: WP_375741062.1
• Uniprot ID: -
• Organism: Pseudomonas boanensis strain L337
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2153158..2164303

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OXH74_RS09865 (OXH74_09850)	-	2154434..2154730 (-)	297	WP_375741058.1	DUF167 domain-containing protein	-
OXH74_RS09870 (OXH74_09855)	-	2154736..2155329 (-)	594	WP_375741059.1	YggT family protein	-
OXH74_RS09875 (OXH74_09860)	proC	2155340..2156158 (-)	819	WP_375741060.1	pyrroline-5-carboxylate reductase	-
OXH74_RS09880 (OXH74_09865)	-	2156172..2156864 (-)	693	WP_375741061.1	YggS family pyridoxal phosphate-dependent enzyme	-
OXH74_RS09885 (OXH74_09870)	pilT	2157075..2158109 (+)	1035	WP_215377493.1	type IV pilus twitching motility protein PilT	Machinery gene
OXH74_RS09890 (OXH74_09875)	pilU	2158158..2159303 (+)	1146	WP_375741062.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OXH74_RS09895 (OXH74_09880)	-	2159364..2159759 (+)	396	WP_375741063.1	NINE protein	-
OXH74_RS09900 (OXH74_09885)	-	2160056..2161435 (+)	1380	WP_375741064.1	pyridoxal-phosphate dependent enzyme	-
OXH74_RS09905 (OXH74_09890)	-	2161432..2162625 (+)	1194	WP_375741065.1	trans-sulfuration enzyme family protein	-
OXH74_RS09910 (OXH74_09895)	-	2162930..2163787 (-)	858	WP_215377513.1	glycine betaine ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42579.90 Da
• Isoelectric Point: 6.7791

>NTDB_id=763354 OXH74_RS09890 WP_375741062.1 2158158..2159303(+) (pilU) [Pseudomonas boanensis strain L337]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKILPVTKNSMSPEMTRETVLAVMNEQQRREFAQNHECNFAISARGIGR
FRVSAFYQRNLIGMVLRRIETNIPTIDDLKLPEVLKQLAMTKRGLVLFVGATGTGKSTSLAAMIGHRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAIAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLIATPDGKGRRAVIEVLINTPLVADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALVHADSANDLRLMIKLGSETDGEHLTHMSDGLSLQTSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=763354 OXH74_RS09890 WP_375741062.1 2158158..2159303(+) (pilU) [Pseudomonas boanensis strain L337]
ATGGAATTCGAGAAACTGCTGCGCCTGATGGTTGAAAAAGGTGGATCGGACCTCTTCATCACGGCAGGTGTGCCGCCTTC
TATGAAGGTCAACGGCAAGATCCTGCCTGTGACCAAGAACTCCATGTCGCCGGAGATGACGCGGGAAACCGTCCTGGCGG
TGATGAACGAGCAACAGCGCCGTGAGTTCGCACAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGAGGCATTGGCCGT
TTCCGCGTCAGCGCCTTCTATCAGCGCAACCTGATCGGCATGGTGCTGCGCCGGATCGAGACGAACATTCCCACCATCGA
TGACCTGAAGCTGCCGGAGGTCCTCAAGCAACTGGCAATGACCAAACGCGGCCTGGTGCTGTTCGTCGGCGCTACCGGCA
CCGGCAAGTCCACGTCCCTGGCGGCGATGATCGGCCATCGCAACAAGAACAGCAGCGGTCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAGCTGCATCGTCACCCAGCGCGAAGTGGGTATCGATACCGAATCGTTCGAGGT
GGCCCTGAAGAACACCCTGCGTCAGGCGCCAGACGTGATTCTCATCGGCGAAGTGCGAACCCGCGAGACGATGGATCATG
CCATTGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCATGCCAACAATGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCTGCCGACCGTCAGCAGCAGGTCTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
GCTGATTGCGACGCCGGATGGCAAGGGCCGCCGCGCGGTGATCGAAGTGCTGATCAACACCCCGCTGGTCGCGGACCTGA
TCCGCAAAGGCGAGGTCCACGAACTCAAACCATTGATGAAACGATCCACCGAGCAGGGCATGCAGACGTTCGACCAGGCG
CTCTACAATCTCTACTCGCAGGGCGAGATCACCTACGAGGACGCGCTGGTCCACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTGGGTTCGGAGACCGATGGCGAGCACCTGACGCACATGAGCGATGGCCTGTCGTTGCAGACCAGTGAAG
AGGACCCCGGCCGACGGTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	90.814	100	0.908
pilU	Acinetobacter baylyi ADP1	65.546	93.701	0.614
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.976	0.383