Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OXH74_RS09885 Genome accession   NZ_CP113889
Coordinates   2157075..2158109 (+) Length   344 a.a.
NCBI ID   WP_215377493.1    Uniprot ID   -
Organism   Pseudomonas boanensis strain L337     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2152075..2163109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OXH74_RS09860 (OXH74_09845) - 2152236..2154224 (-) 1989 WP_375741057.1 dynamin family protein -
  OXH74_RS09865 (OXH74_09850) - 2154434..2154730 (-) 297 WP_375741058.1 DUF167 domain-containing protein -
  OXH74_RS09870 (OXH74_09855) - 2154736..2155329 (-) 594 WP_375741059.1 YggT family protein -
  OXH74_RS09875 (OXH74_09860) proC 2155340..2156158 (-) 819 WP_375741060.1 pyrroline-5-carboxylate reductase -
  OXH74_RS09880 (OXH74_09865) - 2156172..2156864 (-) 693 WP_375741061.1 YggS family pyridoxal phosphate-dependent enzyme -
  OXH74_RS09885 (OXH74_09870) pilT 2157075..2158109 (+) 1035 WP_215377493.1 type IV pilus twitching motility protein PilT Machinery gene
  OXH74_RS09890 (OXH74_09875) pilU 2158158..2159303 (+) 1146 WP_375741062.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OXH74_RS09895 (OXH74_09880) - 2159364..2159759 (+) 396 WP_375741063.1 NINE protein -
  OXH74_RS09900 (OXH74_09885) - 2160056..2161435 (+) 1380 WP_375741064.1 pyridoxal-phosphate dependent enzyme -
  OXH74_RS09905 (OXH74_09890) - 2161432..2162625 (+) 1194 WP_375741065.1 trans-sulfuration enzyme family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37856.63 Da        Isoelectric Point: 6.8360

>NTDB_id=763353 OXH74_RS09885 WP_215377493.1 2157075..2158109(+) (pilT) [Pseudomonas boanensis strain L337]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEDLGMGEIFKKVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVAKGLVSRESAREKAKSPESL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=763353 OXH74_RS09885 WP_215377493.1 2157075..2158109(+) (pilT) [Pseudomonas boanensis strain L337]
ATGGACATTACTGAGCTGCTTGCCTTCAGCGCCAAGCAGGGCGCTTCGGACCTGCACCTCTCCGCTGGCCTGCCGCCGAT
GATCCGCGTGGATGGCGACGTGCGTCGAATCAACCTGCCCGCCCTCGATCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGATTACGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTGCCGGGCGTTGCCCGC
TTCCGGGTCAATGCCTTCAACCAGAACCGTGGTTCGGGCGCGGTGTTCCGGACCATTCCGTCCAAGGTCCTGACCATGGA
AGACCTGGGGATGGGCGAGATCTTCAAGAAGGTGTCCGATGTGCCGCGCGGCCTGGTGCTGGTCACCGGGCCCACGGGTT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTCAACAGCACCAAGTATCACCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTTCATGAGTCGAAGAAATGCCTGATCAACCAGCGCGAGGTCCATCGCGACACCCTCGGCTTCAACGA
GGCGCTGCGCTCGGCACTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGGGACCTGGAAACCATCCGTCTGG
CCCTGACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACAACCTCCGCGGCGAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCGGAAGAAAAGTCCATGGTTCGCTCCATGCTGTCCGAATCCCTGCAGGCGGTGATTTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGTGTGGCAGCCCACGAGATCATGATCGGCACGCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGTGGCTCTCTCGGCATGCAAACCCTCGACATGTGCCTC
AAGGGTCTGGTGGCCAAGGGGCTGGTCAGCCGTGAAAGTGCCCGGGAAAAGGCCAAGAGTCCGGAAAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

94.477

100

0.945
  pilT Pseudomonas stutzeri DSM 10701

93.605

100

0.936
  pilT Acinetobacter baumannii D1279779

86.047

100

0.86
  pilT Acinetobacter baumannii strain A118

86.047

100

0.86
  pilT Acinetobacter nosocomialis M2

85.756

100

0.858
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.42

100

0.395