Detailed information    

experimental Experimentally validated

Overview

Name   clpA   Type   Unclear
Locus tag   Cj1108 Genome accession   NC_002163
Coordinates   1040450..1042579 (+) Length   709 a.a.
NCBI ID   YP_002344501.1    Uniprot ID   Q0P9E6
Organism   Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819     
Function   require for natural transformation   
Unclear

Function

This showed the ΔclpP mutant of C. jejuni to be practically untransformable, while mutants lacking either the ClpX or the ClpA ATPases showed approximately 93 and 80% reduced transformation, respectively, when compared to the wild-type (wt) strain


Genomic Context

Location: 1035450..1047579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cj1102 truB 1037298..1038116 (+) 819 YP_002344495.1 tRNA pseudouridine synthase B -
  Cj1103 csrA 1038110..1038337 (+) 228 YP_002344496.1 carbon storage regulator -
  Cj1104 - 1038334..1039101 (+) 768 YP_002344497.1 4-diphosphocytidyl-2C-methyl-D-erythritol kinase -
  Cj1105 smpB 1039098..1039550 (+) 453 YP_002344498.1 SsrA-binding protein -
  Cj1106 - 1039561..1040163 (+) 603 YP_002344499.1 thioredoxin -
  Cj1107 clpS 1040163..1040453 (+) 291 YP_002344500.1 ATP-dependent Clp protease adaptor protein -
  Cj1108 clpA 1040450..1042579 (+) 2130 YP_002344501.1 ATP-dependent Clp protease ATP-binding subunit Unclear
  Cj1109 aat 1042554..1043201 (+) 648 YP_002344502.1 leucyl/phenylalanyl-tRNA--protein transferase -
  Cj1110c - 1043223..1044512 (-) 1290 YP_002344503.1 MCP-type signal transduction protein -
  Cj1111c - 1044593..1045219 (-) 627 YP_002344504.1 MarC family integral membrane protein -
  Cj1112c - 1045234..1045593 (-) 360 YP_002344505.1 methionine sulfoxide reductase B -
  Cj1113 - 1045683..1046480 (+) 798 YP_002344506.1 hypothetical protein -
  Cj1114c pssA 1046471..1047199 (-) 729 YP_002344507.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -

Sequence

Protein


Download         Length: 709 a.a.        Molecular weight: 80414.60 Da        Isoelectric Point: 7.2357

>NTDB_id=1474 Cj1108 YP_002344501.1 1040450..1042579(+) (clpA) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
MKYQENLQKYLDNAKNLSLINHHEFVTCEHVLFALLKLSTDFKDIFEEFSDGDLELLETELKNYISQNNQVIKQEIEPTI
SVVLDEILLSSKNKNNEIKIIDFLEKLIQDSRSYSSYLLKKHNINLNKIQELQNHENIQNLNNHTSDLTLLAQNGKIDPL
IGRKFELERMMQILSRRKKNNPILVGEAGVGKTAIVEGLALAIAEKKVPKNLQNAKIFSLDMASILAGTKYRGDFEKRIK
EILSELEKIPNAILFIDEIHTIVGAGSTGESHTDFSNLLKPALSNGTLKCIGATTFMEYKNTFDKNKPLSRRFAKINVDE
PSQEESLQILKGLKNKYEEFHHIKLNDEILQYAVIWGKKFFNDKFLPDSAIDLIDELGASFALNPRAKKNANLKDLENVL
AKMTHHHKMFEFDQNKALMNLKTNLKAKIFGQDEVIDSLVSSLKQSFAGFKNSNTPRGVFLFTGSSGVGKTELCKALAEF
LGLNLERFDMSEYAEKHAISKLIGSPAGYIGFEEGGLLSNAIRKNPFSLVLFDEIEKAHPDLSNTFLQIFDNAELTDNSG
LKVDFKNTIIIMTSNLGLKESNELGFLSKNEEKSNRAIKDFFAPEFINRIDKILHFNDLNDAILVKIIEKELDEISKNLN
NIKLSVDDKAKIYLAKKAYNKEFGVRLLKRIISEEIGEKISDEILFGKLKKGGIAKIKLGKNEKLEFIF

Nucleotide


Download         Length: 2130 bp        

>NTDB_id=1474 Cj1108 YP_002344501.1 1040450..1042579(+) (clpA) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
ATGAAATACCAAGAAAATTTACAAAAATATCTTGATAATGCCAAAAATCTAAGCTTAATAAATCACCATGAATTTGTTAC
CTGTGAGCATGTACTGTTTGCATTACTAAAACTAAGCACTGATTTTAAGGATATATTTGAAGAATTTTCAGATGGAGATC
TTGAACTTCTAGAAACTGAATTAAAAAATTACATTTCTCAAAACAATCAAGTTATTAAACAAGAAATAGAACCTACAATA
TCTGTTGTTTTAGATGAAATCTTACTTTCATCTAAAAATAAAAATAATGAAATTAAAATTATAGATTTTTTAGAAAAGCT
CATACAGGATTCAAGAAGTTATTCTAGCTATCTCTTAAAAAAACACAACATAAATCTTAACAAAATTCAAGAACTTCAAA
ATCATGAAAATATACAAAATTTAAACAACCACACAAGTGATTTAACACTTTTAGCTCAAAATGGCAAAATTGATCCTTTA
ATAGGAAGAAAATTTGAACTTGAAAGAATGATGCAAATTCTTTCACGTCGCAAGAAAAATAATCCTATTTTAGTAGGAGA
AGCAGGAGTTGGAAAAACAGCCATTGTAGAGGGATTAGCATTGGCTATTGCGGAAAAAAAGGTGCCTAAAAACTTACAAA
ATGCAAAAATTTTTAGTCTTGATATGGCTAGTATACTTGCGGGAACAAAATACCGTGGAGATTTTGAGAAAAGAATTAAA
GAAATTTTAAGTGAACTTGAGAAAATACCCAATGCTATTTTATTTATTGATGAAATTCACACTATAGTAGGAGCTGGAAG
CACAGGAGAATCTCATACTGATTTTTCCAATCTTTTAAAACCAGCTCTAAGCAATGGAACTTTAAAATGCATAGGTGCAA
CAACTTTTATGGAATATAAAAATACTTTTGATAAAAATAAACCCCTAAGTCGCCGTTTTGCTAAAATAAATGTAGATGAA
CCAAGCCAAGAAGAAAGCTTGCAAATTCTTAAAGGATTAAAAAACAAATATGAAGAATTTCATCATATCAAATTAAACGA
TGAAATTCTTCAATATGCTGTAATTTGGGGTAAAAAATTTTTTAATGATAAATTTTTACCTGATAGCGCTATAGATCTGA
TCGATGAACTTGGCGCTTCTTTTGCTTTAAATCCAAGAGCTAAAAAAAATGCCAATTTAAAAGATTTAGAAAATGTTTTA
GCAAAAATGACCCACCATCACAAAATGTTTGAATTCGATCAAAATAAAGCTTTAATGAACTTAAAAACAAATTTAAAAGC
AAAAATTTTTGGCCAAGATGAAGTTATAGATAGCCTTGTTTCATCACTTAAGCAAAGTTTCGCAGGATTTAAAAATTCCA
ATACACCACGCGGAGTGTTTTTGTTTACTGGATCAAGTGGAGTTGGAAAAACAGAACTTTGCAAAGCTTTAGCTGAATTT
TTAGGTTTAAATTTAGAACGCTTTGATATGAGTGAATATGCAGAAAAACATGCTATAAGTAAACTAATAGGATCTCCAGC
TGGATATATAGGTTTTGAAGAGGGTGGACTTTTAAGCAATGCTATTCGCAAAAATCCTTTCAGTCTTGTTCTTTTTGACG
AAATAGAAAAGGCTCATCCTGATTTAAGCAATACCTTTTTACAGATTTTTGACAATGCTGAACTCACCGACAATAGTGGT
CTTAAAGTAGATTTTAAAAATACAATCATTATTATGACATCTAATCTAGGACTTAAAGAAAGCAATGAGCTTGGTTTTTT
AAGTAAAAATGAAGAAAAAAGTAATCGTGCTATCAAAGACTTTTTTGCTCCAGAATTTATTAATCGCATTGATAAAATTT
TACATTTTAATGATTTAAACGATGCTATACTTGTTAAAATTATCGAAAAAGAACTTGACGAAATTTCTAAAAATTTAAAT
AATATAAAATTATCAGTGGATGATAAAGCTAAAATTTATTTAGCTAAAAAAGCCTATAATAAAGAATTTGGAGTAAGGCT
TTTAAAACGCATAATTTCTGAGGAAATAGGAGAAAAAATCAGTGATGAAATTTTATTTGGAAAGCTTAAAAAAGGTGGTA
TAGCTAAAATCAAACTTGGTAAAAATGAAAAGCTCGAATTTATATTCTAA

Domains

Predicted by InterproScan.

AAA lid domain (AAA_lid_9)

(322-419)

Clp amino terminal domain, pathogenicity island component (Clp_N)

(14-63)

AAA domain (Cdc48 subfamily) (AAA_2)

(456-613)

ATPase family associated with various cellular activities (AAA) (AAA)

(183-296)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(619-696)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0P9E6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpC Streptococcus mutans UA159

38.089

100

0.41
  clpC Bacillus subtilis subsp. subtilis str. 168

36.237

100

0.405
  clpC Streptococcus pneumoniae Rx1

36.541

100

0.396
  clpC Streptococcus pneumoniae D39

36.541

100

0.396
  clpC Streptococcus pneumoniae TIGR4

36.541

100

0.396
  clpC Streptococcus thermophilus LMG 18311

42.072

89.845

0.378
  clpC Streptococcus thermophilus LMD-9

41.89

89.563

0.375
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

34.555

100

0.372

Multiple sequence alignment    



References

[1] Christina S Vegge et al. (2012) Natural transformation of Campylobacter jejuni occurs beyond limits of growth. PloS One 7(9):e45467. [PMID: 23049803]