Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OY559_RS15770 Genome accession   NZ_CP113783
Coordinates   3335273..3337006 (+) Length   577 a.a.
NCBI ID   WP_277727179.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. SE1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3330273..3342006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OY559_RS15750 (OY559_15760) - 3330448..3332070 (+) 1623 WP_277727175.1 ATP-binding protein -
  OY559_RS15755 (OY559_15765) - 3332302..3333706 (+) 1405 Protein_3095 sigma-54 dependent transcriptional regulator -
  OY559_RS15760 (OY559_15770) - 3334150..3334242 (+) 93 Protein_3096 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OY559_RS15765 - 3334783..3335112 (+) 330 WP_277727177.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OY559_RS15770 (OY559_15780) pilB 3335273..3337006 (+) 1734 WP_277727179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OY559_RS15775 (OY559_15785) pilC 3337373..3338638 (+) 1266 WP_277727181.1 type II secretion system F family protein Machinery gene
  OY559_RS15780 (OY559_15790) pilD 3338642..3339505 (+) 864 WP_277727182.1 A24 family peptidase Machinery gene
  OY559_RS15785 (OY559_15795) coaE 3339590..3340237 (+) 648 WP_277727185.1 dephospho-CoA kinase -
  OY559_RS15790 (OY559_15800) - 3340395..3340688 (-) 294 WP_277727187.1 transposase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62902.11 Da        Isoelectric Point: 6.0039

>NTDB_id=763044 OY559_RS15770 WP_277727179.1 3335273..3337006(+) (pilB) [Pseudoxanthomonas sp. SE1]
MNAAASVNLVGITGLARRLVQDGALDETIARAAMTSAAEAKIPLPQWIAEKRLVTAPQLAAANAMEFGMPLLDATAFDPT
HSALKLVSEELVQKHQVLPLFKRGNKLFVGVADPTRSHGALDEIKFHTNLIVEPVLVDEDQLRRTLDQWQNAANTLSSNF
GDDDDGLESLEVDASDEDGGTDNAVDSKGDDTPVVKFVNKVLVDAIKRGASDIHFEPYETDYRVRLRVDGILKQVAKAPT
KLKDRIAARIKVMAQLDIAEKRVPQDGRIKLNLSKSKQMDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FEDAIQKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNRRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLIIAQRLARRLCSKCKRPVQLPP
QALLAEGFSEAEIAAGFTVYEAVGCDECTSGYKGRTGIYQVMPMNDAIQEIVLEGGNAMQIAEKAQKAGIRDLRQSALMK
VRNGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=763044 OY559_RS15770 WP_277727179.1 3335273..3337006(+) (pilB) [Pseudoxanthomonas sp. SE1]
ATGAATGCAGCTGCCAGTGTCAATCTCGTTGGCATCACCGGTCTTGCACGCCGCTTGGTTCAAGACGGGGCCCTCGACGA
AACCATCGCCCGGGCCGCGATGACAAGCGCTGCCGAAGCCAAGATTCCGTTGCCTCAATGGATAGCCGAGAAGCGGTTGG
TCACCGCGCCGCAATTGGCAGCCGCCAATGCCATGGAATTCGGCATGCCGCTGCTGGATGCAACGGCATTCGACCCGACC
CACAGCGCACTCAAGCTGGTCAGCGAGGAACTGGTGCAGAAGCACCAGGTGCTCCCCCTCTTCAAACGCGGCAACAAGCT
CTTCGTCGGTGTCGCCGACCCCACCCGCAGCCATGGCGCACTGGACGAGATCAAGTTCCACACCAACCTGATCGTCGAAC
CGGTGCTGGTGGACGAGGACCAGCTGCGCCGCACCCTGGACCAGTGGCAGAACGCCGCCAACACCCTGTCCAGCAACTTC
GGCGACGACGACGACGGGCTGGAAAGCCTGGAGGTGGATGCTTCCGATGAGGACGGCGGTACCGACAATGCGGTCGACTC
GAAGGGCGATGACACGCCGGTCGTGAAGTTCGTCAACAAGGTCCTGGTGGATGCCATAAAGCGCGGCGCCTCGGACATCC
ACTTCGAGCCGTACGAGACCGACTACCGCGTGCGGCTGCGCGTGGACGGCATCCTGAAGCAGGTCGCCAAGGCGCCGACC
AAGCTGAAGGACCGCATTGCCGCGCGCATCAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTACCGCAGGACGG
CCGCATCAAGCTGAACCTGTCCAAGAGCAAGCAGATGGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTGGACGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCCGACCAGCAGAAGCTG
TTCGAGGATGCCATCCAGAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACCGTATCGCTGTA
CACCGCACTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TGAACCAGGTGCAGCAGAACAACCGCCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCACACCAACGACGCACCGCAGACCATCGCCCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCA
GCTCGGTGACCCTGATCATCGCGCAACGCCTGGCACGCCGGCTGTGTTCGAAGTGCAAGCGCCCGGTGCAACTGCCCCCG
CAGGCGCTGCTGGCGGAAGGCTTCAGCGAGGCCGAGATCGCCGCCGGCTTCACCGTTTACGAGGCCGTGGGCTGCGACGA
GTGCACCAGTGGCTACAAGGGCCGTACCGGCATCTACCAGGTCATGCCGATGAACGACGCGATCCAGGAGATCGTGCTGG
AAGGCGGCAATGCCATGCAGATCGCGGAAAAGGCCCAGAAAGCCGGCATCCGCGACCTGCGCCAGTCGGCGTTGATGAAA
GTGCGCAACGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.497

98.267

0.536
  pilB Acinetobacter baylyi ADP1

53.968

98.267

0.53
  pilB Legionella pneumophila strain ERS1305867

53.521

98.44

0.527
  pilF Neisseria gonorrhoeae MS11

49.825

99.133

0.494
  pilB Vibrio cholerae strain A1552

49.559

98.267

0.487
  pilB Vibrio campbellii strain DS40M4

46.316

98.787

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

46.397

98.614

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.115

90.295

0.362