Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OXA86_RS04860 Genome accession   NZ_CP113439
Coordinates   1063284..1063883 (+) Length   199 a.a.
NCBI ID   WP_014841277.1    Uniprot ID   A0AAV2UVV9
Organism   Legionella pneumophila strain PATHC002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1058284..1068883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OXA86_RS04845 (OXA86_04845) ravI 1060230..1061237 (-) 1008 WP_014841275.1 Dot/Icm T4SS effector RavI -
  OXA86_RS04850 (OXA86_04850) pilM 1061654..1062718 (+) 1065 WP_014841276.1 type IV pilus assembly protein PilM Machinery gene
  OXA86_RS04855 (OXA86_04855) pilN 1062722..1063270 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  OXA86_RS04860 (OXA86_04860) pilO 1063284..1063883 (+) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  OXA86_RS04865 (OXA86_04865) pilP 1063880..1064464 (+) 585 WP_014841278.1 pilus assembly protein PilP Machinery gene
  OXA86_RS04870 (OXA86_04870) pilQ 1064468..1066564 (+) 2097 WP_044497174.1 type IV pilus secretin PilQ Machinery gene
  OXA86_RS04875 (OXA86_04875) aroK 1067009..1067536 (+) 528 WP_010946667.1 shikimate kinase AroK -
  OXA86_RS04880 (OXA86_04880) aroB 1067523..1068632 (+) 1110 WP_014841280.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22496.21 Da        Isoelectric Point: 5.3369

>NTDB_id=762229 OXA86_RS04860 WP_014841277.1 1063284..1063883(+) (pilO) [Legionella pneumophila strain PATHC002]
MDTVNLSELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=762229 OXA86_RS04860 WP_014841277.1 1063284..1063883(+) (pilO) [Legionella pneumophila strain PATHC002]
ATGGACACGGTCAATTTAAGTGAATTAACTCTGGAGAATGTCGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTCATTTT
AGGTATCAGTATACTGATAATTGGATTAGGATATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAATCGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCTTATCGTAATCAGTTA
CAGTTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTCCCGGCGAAAAATGAAATGCCAGGGCTTTTGGAAGAAAT
ATCTAAAACGGGGATTGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCACGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTAGCTGAAATGAACAGA
ATAGTGACCTTGCATGAATTTAGTGTTGAGGGGGTATCATCCGGGGATGGTAAAACGGTCTCTCAGGATGAATTGGTGAT
GGCTATCACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

99.497

100

0.995
  comO Acinetobacter baylyi ADP1

39.894

94.472

0.377
  pilO Acinetobacter baumannii D1279779

37.696

95.98

0.362