Detailed information NTG0762019

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: OXA89_RS04890
• Genome accession: NZ_CP113434
• Coordinates: 1070581..1071645 (+)
• Length: 354 a.a.
• NCBI ID: WP_013101265.1
• Uniprot ID: A0A378K321
• Organism: Legionella pneumophila strain PATHC027
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1065581..1076645

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OXA89_RS04880 (OXA89_04880)	-	1066623..1069007 (-)	2385	WP_011946019.1	penicillin-binding protein 1A	-
OXA89_RS04885 (OXA89_04885)	-	1069157..1070164 (-)	1008	WP_013101264.1	hypothetical protein	-
OXA89_RS04890 (OXA89_04890)	pilM	1070581..1071645 (+)	1065	WP_013101265.1	type IV pilus assembly protein PilM	Machinery gene
OXA89_RS04895 (OXA89_04895)	pilN	1071649..1072197 (+)	549	WP_013101266.1	PilN domain-containing protein	Machinery gene
OXA89_RS04900 (OXA89_04900)	pilO	1072211..1072810 (+)	600	WP_013101267.1	type 4a pilus biogenesis protein PilO	Machinery gene
OXA89_RS04905 (OXA89_04905)	pilP	1072807..1073391 (+)	585	WP_013101268.1	pilus assembly protein PilP	Machinery gene
OXA89_RS04910 (OXA89_04910)	pilQ	1073395..1075491 (+)	2097	WP_011946023.1	type IV pilus secretin PilQ	Machinery gene
OXA89_RS04915 (OXA89_04915)	aroK	1075938..1076465 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38829.84 Da
• Isoelectric Point: 5.0367

>NTDB_id=762019 OXA89_RS04890 WP_013101265.1 1070581..1071645(+) (pilM) [Legionella pneumophila strain PATHC027]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPTSALEGNMIKDVDVVANCIKKLVERLRFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKIVDVESYAVERSVQQIAQELPAGGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINNDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=762019 OXA89_RS04890 WP_013101265.1 1070581..1071645(+) (pilM) [Legionella pneumophila strain PATHC027]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTTTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGGGAAGAAATACCAACCTCTGCTCTTGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAGGTTTACATCTAAAAATGTGGCATTG
GCTGTGCCTGATTCAGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGATTGACTGATCTTGAAATGGAGGAGCTTGT
CGTTATTGAGGCGGATAAGTATATCCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGTTCATCACTGCTTGATGTTTTAATTGTGGCTTCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCTCATAGA
GCAGGGTTGGAACCTAAAATTGTTGACGTTGAATCTTATGCAGTAGAGCGCTCAGTACAACAAATTGCGCAAGAATTACC
AGCTGGGGGACAAGACAAAATTGTGGCAATAGTAGATATTGGAGCCAATTATACTCACTTGTTTGTTTTGCACGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGTGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGTTTTGTAGACCATATTTTATTGG
CAGGAGGAATGGCCAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAACCCG
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAATGATGCGCCTACACTAATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A378K321

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	98.588	100	0.986
comM	Acinetobacter nosocomialis M2	42.938	100	0.429
pilM	Acinetobacter baumannii D1279779	42.655	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418