Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OSV15_RS03075 Genome accession   NZ_CP113257
Coordinates   662465..664555 (+) Length   696 a.a.
NCBI ID   WP_267932049.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain TF18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 657465..669555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSV15_RS03055 (OSV15_03055) comM 659631..660695 (+) 1065 WP_102839584.1 pilus assembly protein PilM Machinery gene
  OSV15_RS03060 (OSV15_03060) - 660695..661267 (+) 573 WP_267932047.1 PilN domain-containing protein -
  OSV15_RS03065 (OSV15_03065) pilO 661264..661887 (+) 624 WP_267932048.1 type 4a pilus biogenesis protein PilO -
  OSV15_RS03070 (OSV15_03070) pilP 661884..662411 (+) 528 WP_125868795.1 type 4a pilus biogenesis lipoprotein PilP -
  OSV15_RS03075 (OSV15_03075) pilQ 662465..664555 (+) 2091 WP_267932049.1 type IV pilus secretin PilQ Machinery gene
  OSV15_RS03080 (OSV15_03080) aroK 664561..665106 (+) 546 WP_267932938.1 shikimate kinase AroK -
  OSV15_RS03085 (OSV15_03085) aroB 665129..666232 (+) 1104 WP_267932050.1 3-dehydroquinate synthase -
  OSV15_RS03090 (OSV15_03090) - 666243..667808 (+) 1566 WP_063539619.1 AAA family ATPase -

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 74856.45 Da        Isoelectric Point: 4.6189

>NTDB_id=761301 OSV15_RS03075 WP_267932049.1 662465..664555(+) (pilQ) [Stutzerimonas frequens strain TF18]
MNSTLSRLGISMLVAFVSPAVLAANLNALDVAALPGDRVELKLAFDEPVAPPRGYTLEQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGKNLFVVLGENAGASGPAAVSAQPSAVAHPSVATAPAKAVTVGKA
IDNIDFRRGDDGAGNVIVTLSDPSISSTIEEQGGKIRVNFAKAQLPESLRVRLDVQDFATPVRFVDSASQAGGASIAIEP
TGRYDYLAYQTDNKLTISVKPLTEDEAEKRKADRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRL
QNVPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTNT
QGETEDRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKALGVRWGGTQLFANGRGAVY
GNDDFGDEGGDSGEESSGNFPFVDMGVANRTAGIGIGYITDNLILDLELSAMEKSGNGEVVSQPKVMTADKETAKILKGS
EIPYQEASSSGATSTTFVEAALSLEVTPQITPDNRIIMEVKVTKDEPDFANDVNGTPTIRKNEVNAKILVNDGETVVIGG
VFSNTQSKSVDKVPFLGDLPYLGRMFRRDVVADSKSELLIFLTPRILNHQAVAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=761301 OSV15_RS03075 WP_267932049.1 662465..664555(+) (pilQ) [Stutzerimonas frequens strain TF18]
ATGAACAGTACTCTCTCGCGTCTCGGCATTTCAATGCTTGTGGCGTTCGTCTCGCCGGCTGTGCTGGCAGCCAATCTCAA
TGCCTTGGATGTGGCCGCTCTGCCGGGCGATCGAGTCGAGCTCAAGCTGGCTTTCGACGAGCCCGTTGCGCCGCCGCGTG
GTTACACCCTTGAACAGCCTGCTCGTATTGCCCTTGATCTACCGGGTGTATCGAACAACCTAGGGGCGAAGAATCGTGAG
CTGGGGGTTGGTAATGCGCGTAGCGTTACGGTCGTTGAGGCGCAAGGACGTACTCGGTTGATCGTGAACCTCACTTCGCT
GGTGCCCTATTCCACTCGAGTGGATGGCAAGAACCTGTTCGTCGTGCTGGGCGAAAATGCCGGCGCGAGTGGCCCAGCAG
CGGTCTCAGCGCAGCCGAGCGCAGTGGCGCATCCATCTGTTGCGACGGCTCCCGCGAAGGCGGTGACTGTTGGTAAGGCC
ATTGACAACATTGACTTTCGGCGTGGCGATGATGGGGCTGGTAATGTGATTGTTACACTATCCGACCCATCAATCAGCTC
GACCATTGAAGAGCAGGGCGGAAAAATTCGCGTAAATTTCGCCAAGGCTCAGTTGCCGGAATCGCTGCGTGTGCGCCTGG
ATGTCCAAGACTTCGCTACGCCTGTCAGGTTCGTTGATTCCGCTTCTCAAGCAGGTGGTGCGAGCATCGCAATCGAGCCG
ACTGGTCGATATGACTATTTGGCCTATCAGACCGACAACAAGCTGACTATCAGCGTCAAACCTCTTACGGAAGACGAAGC
GGAAAAGCGTAAGGCTGATCGATTTGCTTATTCTGGCGAGAAGCTGTCGCTTAATTTCCAAGACATCGACGTTCGCTCGG
TATTGCAGCTAATCGCTGATTTCACCGATCTAAATCTGGTCGCAAGCGATACCGTTAGCGGCAATATCACGCTGCGTCTG
CAGAACGTGCCGTGGGATCAGGCTCTGGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCCAGGTCGGTAACGTGCT
ACTGGTCGCTCCTGCCGACGAAATTGCCGCACGTGAACGTCAGGAGCTTGAGTCTCAGCGGCAGATTGCCGAACTGGCTC
CGCTTCGCCGTGAGGTGGTGCAGGTTAACTACGCCAAGGCGGCGGATATCGCTCGCCTTTTCCAGTCTGTAACCAATACC
CAGGGCGAAACCGAGGACCGCGGTTCGATAGCCGTTGATGATAGAACCAACAATATTATTGCCTACCAGACGCAAGAGCG
TCTGGACGAGCTACGGCGGATCGTCGCGCAACTCGACATCCCTGTCCGGCAGGTCATGATCGAGGCTCGAATTGTTGAAG
CGAACGTGGATTATGATAAAGCGCTGGGCGTGCGCTGGGGCGGCACGCAGCTTTTTGCCAACGGGCGGGGCGCTGTCTAT
GGCAACGACGATTTTGGCGATGAAGGTGGAGACTCAGGGGAAGAAAGCAGCGGTAATTTTCCTTTCGTCGATATGGGCGT
CGCTAACCGTACTGCAGGTATCGGCATCGGTTACATTACCGACAACCTGATCCTTGACCTTGAGCTGTCGGCAATGGAGA
AGTCTGGTAACGGCGAGGTCGTGTCGCAGCCTAAGGTAATGACCGCTGACAAGGAAACCGCGAAGATCCTCAAGGGCTCT
GAGATTCCTTACCAAGAGGCCAGTTCAAGTGGCGCTACATCGACTACCTTTGTCGAGGCAGCTCTATCGCTGGAGGTTAC
GCCGCAGATCACTCCAGATAATCGCATCATCATGGAAGTCAAAGTGACCAAGGATGAGCCCGACTTTGCCAACGATGTGA
ACGGAACTCCTACCATTCGCAAAAACGAGGTGAATGCAAAGATTCTGGTGAATGACGGTGAGACAGTTGTCATCGGAGGC
GTCTTCTCCAACACCCAGAGCAAGTCAGTAGATAAAGTTCCTTTCTTGGGTGATCTGCCTTATCTTGGACGGATGTTCCG
CCGAGATGTTGTGGCGGACTCGAAGTCCGAATTGCTGATATTTCTGACTCCCAGAATACTCAATCATCAGGCCGTCGCTG
TAAGCCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II and III secretion system protein (Secretin)

(531-687)

AMIN domain (AMIN)

(38-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.766

100

0.741
  comQ Acinetobacter baylyi ADP1

41.643

99.713

0.415
  pilQ Acinetobacter baumannii strain A118

38.995

100

0.412
  pilQ Acinetobacter baumannii D1279779

39.048

100

0.412