Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   OUY23_RS05615 Genome accession   NZ_CP113246
Coordinates   1219974..1220510 (+) Length   178 a.a.
NCBI ID   WP_025921208.1    Uniprot ID   Q8KQ36
Organism   Pseudomonas aeruginosa strain SMC4386     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1214974..1225510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OUY23_RS05585 (OUY23_05585) yacG 1215143..1215343 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  OUY23_RS05590 (OUY23_05590) coaE 1215340..1215951 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  OUY23_RS05595 (OUY23_05595) pilD 1215948..1216820 (-) 873 WP_017148550.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  OUY23_RS05600 (OUY23_05600) pilC 1216823..1218040 (-) 1218 WP_023103506.1 type II secretion system F family protein Machinery gene
  OUY23_RS05605 (OUY23_05605) pilB 1218044..1219747 (-) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OUY23_RS05615 (OUY23_05615) pilE 1219974..1220510 (+) 537 WP_025921208.1 pilin Machinery gene
  OUY23_RS05620 (OUY23_05620) tfpZ 1220575..1221312 (+) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  OUY23_RS05630 (OUY23_05630) nadC 1221546..1222394 (-) 849 WP_033955591.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OUY23_RS05635 (OUY23_05635) - 1222680..1224935 (+) 2256 WP_033955592.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18322.83 Da        Isoelectric Point: 4.9984

>NTDB_id=761135 OUY23_RS05615 WP_025921208.1 1219974..1220510(+) (pilE) [Pseudomonas aeruginosa strain SMC4386]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRVSEGLVLAEPAKLMISTDGSASTADLTRATTTWNQQSNNLGA
SSKYVTSVLMDAGNTGVITITYVADQVGLPTAGNTLILSPYINDGNTRTALATAVAAGTRGTIDWACTSASNATATAQGF
TGMAAGSVPQEFAPAQCR

Nucleotide


Download         Length: 537 bp        

>NTDB_id=761135 OUY23_RS05615 WP_025921208.1 1219974..1220510(+) (pilE) [Pseudomonas aeruginosa strain SMC4386]
ATGAAAGCTCAAAAAGGCTTCACTTTGATCGAGCTGATGATCGTTGTAGCGATCATCGGTATTCTGGCTGCGATTGCTCT
GCCGGCCTATCAGGACTACACCGTTCGTACCCGTGTCAGCGAAGGTCTGGTTCTGGCTGAGCCCGCCAAACTGATGATCT
CCACGGATGGAAGTGCTTCTACTGCTGATCTGACTCGCGCAACCACTACTTGGAACCAGCAATCCAATAATCTGGGTGCA
TCCAGTAAGTACGTAACATCTGTTTTGATGGATGCTGGTAACACCGGTGTTATCACTATTACGTATGTCGCTGACCAAGT
GGGCTTGCCGACTGCCGGTAACACTCTGATTCTGTCTCCCTATATCAATGATGGTAACACTCGCACTGCTCTGGCGACTG
CAGTGGCTGCTGGCACTCGTGGCACCATTGATTGGGCTTGCACTTCGGCATCGAACGCGACTGCCACTGCTCAGGGTTTC
ACCGGCATGGCAGCTGGTAGTGTGCCGCAAGAGTTTGCTCCTGCTCAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3JYZ
  PDB 3JZZ

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.719

100

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

40.556

100

0.41
  pilA2 Legionella pneumophila strain ERS1305867

40.341

98.876

0.399
  pilA2 Legionella pneumophila str. Paris

39.773

98.876

0.393