Detailed information NTG0760700

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: QJ956_RS27285
• Genome accession: NZ_CP124665
• Coordinates: 5887773..5888837 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain 2021CK-01229
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5882773..5893837

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QJ956_RS27260 (QJ956_27260)	aroK	5883308..5883826 (-)	519	WP_003095833.1	shikimate kinase AroK	-
QJ956_RS27265 (QJ956_27265)	pilQ	5883838..5885982 (-)	2145	WP_031686163.1	type 4a pilus secretin PilQ	Machinery gene
QJ956_RS27270 (QJ956_27270)	comL/pilP	5886036..5886560 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
QJ956_RS27275 (QJ956_27275)	pilO	5886557..5887180 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
QJ956_RS27280 (QJ956_27280)	pilN	5887177..5887773 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
QJ956_RS27285 (QJ956_27285)	pilM	5887773..5888837 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
QJ956_RS27290 (QJ956_27290)	-	5889022..5891493 (+)	2472	WP_031686164.1	penicillin-binding protein 1A	-
QJ956_RS27295 (QJ956_27295)	-	5891589..5892857 (-)	1269	WP_003095842.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=760700 QJ956_RS27285 WP_003110888.1 5887773..5888837(-) (pilM) [Pseudomonas aeruginosa strain 2021CK-01229]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=760700 QJ956_RS27285 WP_003110888.1 5887773..5888837(-) (pilM) [Pseudomonas aeruginosa strain 2021CK-01229]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTTTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466